Cytotoxic and genotoxic effects of defence secretion of Ulomoides dermestoides on A549 cells

Ethnopharmacological relevance: Ulomoides dermestoides (Fairmaire, 1893) is a cosmopolitan tenebrionid beetle reared by Argentine people who consume them alive as an alternative medicine in the treatment of different illnesses such as asthma, Parkinson's, diabetes, arthritis, HIV and specially...

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Detalles Bibliográficos
Autores: Crespo, Rosana, Villaverde, Maria Luciana, Girotti, Juan Roberto, Güerci, Alba Mabel, Juarez, Marta Patricia, de Bravo, M.G.
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2011
País:Argentina
Institución:Consejo Nacional de Investigaciones Científicas y Técnicas
Repositorio:CONICET Digital (CONICET)
Idioma:inglés
OAI Identifier:oai:ri.conicet.gov.ar:11336/78831
Acceso en línea:http://hdl.handle.net/11336/78831
Access Level:acceso abierto
Palabra clave:Benzoquinones
Cytotoxicity
Genotoxicity
Pentadecene
Tenebrionidae
Ulomoides Dermestoides
https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
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network_acronym_str AR
network_name_str Argentina
repository_id_str
dc.title.none.fl_str_mv Cytotoxic and genotoxic effects of defence secretion of Ulomoides dermestoides on A549 cells
title Cytotoxic and genotoxic effects of defence secretion of Ulomoides dermestoides on A549 cells
spellingShingle Cytotoxic and genotoxic effects of defence secretion of Ulomoides dermestoides on A549 cells
Crespo, Rosana
Benzoquinones
Cytotoxicity
Genotoxicity
Pentadecene
Tenebrionidae
Ulomoides Dermestoides
https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
title_short Cytotoxic and genotoxic effects of defence secretion of Ulomoides dermestoides on A549 cells
title_full Cytotoxic and genotoxic effects of defence secretion of Ulomoides dermestoides on A549 cells
title_fullStr Cytotoxic and genotoxic effects of defence secretion of Ulomoides dermestoides on A549 cells
title_full_unstemmed Cytotoxic and genotoxic effects of defence secretion of Ulomoides dermestoides on A549 cells
title_sort Cytotoxic and genotoxic effects of defence secretion of Ulomoides dermestoides on A549 cells
dc.creator.none.fl_str_mv Crespo, Rosana
Villaverde, Maria Luciana
Girotti, Juan Roberto
Güerci, Alba Mabel
Juarez, Marta Patricia
de Bravo, M.G.
author Crespo, Rosana
author_facet Crespo, Rosana
Villaverde, Maria Luciana
Girotti, Juan Roberto
Güerci, Alba Mabel
Juarez, Marta Patricia
de Bravo, M.G.
author_role author
author2 Villaverde, Maria Luciana
Girotti, Juan Roberto
Güerci, Alba Mabel
Juarez, Marta Patricia
de Bravo, M.G.
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv Benzoquinones
Cytotoxicity
Genotoxicity
Pentadecene
Tenebrionidae
Ulomoides Dermestoides
https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
topic Benzoquinones
Cytotoxicity
Genotoxicity
Pentadecene
Tenebrionidae
Ulomoides Dermestoides
https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
description Ethnopharmacological relevance: Ulomoides dermestoides (Fairmaire, 1893) is a cosmopolitan tenebrionid beetle reared by Argentine people who consume them alive as an alternative medicine in the treatment of different illnesses such as asthma, Parkinson's, diabetes, arthritis, HIV and specially cancer. Aim of the study: To evaluate the cytotoxicity and DNA damage of the major volatile components released by Ulomoides dermestoides on human lung carcinoma epithelial cell line A549. Materials and methods: The defence compounds of Ulomoides dermestoides were extracted with dichloromethane and analyzed and quantified by capillary gas chromatography. The toxicity effects of the beetle's extract against A549 cell line were evaluated. Cytotoxicity was evaluated by MTT test and Trypan blue assay and genotoxicity was evaluated by the comet assay. The synthetic compounds, individually or combined, were also tested in A549 cells and normal mononuclear human cells. Results: The defence compounds of Ulomoides dermestoides extracted with dichloromethane (methyl-1,4-benzoquinones, ethyl-1,4-benzoquinones and 1-pentadecene as major components) showed cytotoxic activity on A549 cells demonstrated by MTT test and Trypan blue assay, with IC 50 values of 0.26 equivalent/ml and 0.34 equivalent/ml, respectively (1 equivalent = amount of components extracted per beetle). The inhibition of A549 cell proliferation with the synthetic blend (1,4-benzoquinone and 1-pentadecene) or 1,4-benzoquinone alone was similar to that obtained with the insect extract. 1-Pentadecene showed no inhibitory effect. Low doses of insect extract or synthetic blend (0.15 equivalent/ml) inhibited mononuclear cell proliferation by 72.2 ± 2.7% and induced significant DNA damage both in tumor and mononuclear cells. Conclusion: Results of this study demonstrated that defence compounds of Ulomoides dermestoides reduced cell viability and induced DNA damage. We also concluded that the insect benzoquinones are primarily responsible for inducing cytotoxicity and genotoxicity in culture cells.
publishDate 2011
dc.date.none.fl_str_mv 2011-06
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/78831
Crespo, Rosana; Villaverde, Maria Luciana; Girotti, Juan Roberto; Güerci, Alba Mabel; Juarez, Marta Patricia; et al.; Cytotoxic and genotoxic effects of defence secretion of Ulomoides dermestoides on A549 cells; Elsevier Ireland; Journal of Ethnopharmacology; 136; 1; 6-2011; 204-209
0378-8741
CONICET Digital
CONICET
url http://hdl.handle.net/11336/78831
identifier_str_mv Crespo, Rosana; Villaverde, Maria Luciana; Girotti, Juan Roberto; Güerci, Alba Mabel; Juarez, Marta Patricia; et al.; Cytotoxic and genotoxic effects of defence secretion of Ulomoides dermestoides on A549 cells; Elsevier Ireland; Journal of Ethnopharmacology; 136; 1; 6-2011; 204-209
0378-8741
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1016/j.jep.2011.04.056
info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0378874111003084
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier Ireland
publisher.none.fl_str_mv Elsevier Ireland
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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spelling Cytotoxic and genotoxic effects of defence secretion of Ulomoides dermestoides on A549 cellsCrespo, RosanaVillaverde, Maria LucianaGirotti, Juan RobertoGüerci, Alba MabelJuarez, Marta Patriciade Bravo, M.G.BenzoquinonesCytotoxicityGenotoxicityPentadeceneTenebrionidaeUlomoides Dermestoideshttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Ethnopharmacological relevance: Ulomoides dermestoides (Fairmaire, 1893) is a cosmopolitan tenebrionid beetle reared by Argentine people who consume them alive as an alternative medicine in the treatment of different illnesses such as asthma, Parkinson's, diabetes, arthritis, HIV and specially cancer. Aim of the study: To evaluate the cytotoxicity and DNA damage of the major volatile components released by Ulomoides dermestoides on human lung carcinoma epithelial cell line A549. Materials and methods: The defence compounds of Ulomoides dermestoides were extracted with dichloromethane and analyzed and quantified by capillary gas chromatography. The toxicity effects of the beetle's extract against A549 cell line were evaluated. Cytotoxicity was evaluated by MTT test and Trypan blue assay and genotoxicity was evaluated by the comet assay. The synthetic compounds, individually or combined, were also tested in A549 cells and normal mononuclear human cells. Results: The defence compounds of Ulomoides dermestoides extracted with dichloromethane (methyl-1,4-benzoquinones, ethyl-1,4-benzoquinones and 1-pentadecene as major components) showed cytotoxic activity on A549 cells demonstrated by MTT test and Trypan blue assay, with IC 50 values of 0.26 equivalent/ml and 0.34 equivalent/ml, respectively (1 equivalent = amount of components extracted per beetle). The inhibition of A549 cell proliferation with the synthetic blend (1,4-benzoquinone and 1-pentadecene) or 1,4-benzoquinone alone was similar to that obtained with the insect extract. 1-Pentadecene showed no inhibitory effect. Low doses of insect extract or synthetic blend (0.15 equivalent/ml) inhibited mononuclear cell proliferation by 72.2 ± 2.7% and induced significant DNA damage both in tumor and mononuclear cells. Conclusion: Results of this study demonstrated that defence compounds of Ulomoides dermestoides reduced cell viability and induced DNA damage. We also concluded that the insect benzoquinones are primarily responsible for inducing cytotoxicity and genotoxicity in culture cells.Fil: Crespo, Rosana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Bioquímicas de La Plata "Prof. Dr. Rodolfo R. Brenner". Universidad Nacional de la Plata. Facultad de Ciencias Médicas. Instituto de Investigaciones Bioquímicas de La Plata "Prof. Dr. Rodolfo R. Brenner"; ArgentinaFil: Villaverde, Maria Luciana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Bioquímicas de La Plata "Prof. Dr. Rodolfo R. Brenner". Universidad Nacional de la Plata. Facultad de Ciencias Médicas. Instituto de Investigaciones Bioquímicas de La Plata "Prof. Dr. Rodolfo R. Brenner"; ArgentinaFil: Girotti, Juan Roberto. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Bioquímicas de La Plata "Prof. Dr. Rodolfo R. Brenner". Universidad Nacional de la Plata. Facultad de Ciencias Médicas. Instituto de Investigaciones Bioquímicas de La Plata "Prof. Dr. Rodolfo R. Brenner"; ArgentinaFil: Güerci, Alba Mabel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico CONICET- La Plata. Instituto de Genética Veterinaria "Ing. Fernando Noel Dulout". Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Instituto de Genética Veterinaria; Argentina. Centro de Investigación de Transferencia en Oncología Molecular Argentina; ArgentinaFil: Juarez, Marta Patricia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Bioquímicas de La Plata "Prof. Dr. Rodolfo R. Brenner". Universidad Nacional de la Plata. Facultad de Ciencias Médicas. Instituto de Investigaciones Bioquímicas de La Plata "Prof. Dr. Rodolfo R. Brenner"; ArgentinaFil: de Bravo, M.G.. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Bioquímicas de La Plata "Prof. Dr. Rodolfo R. Brenner". Universidad Nacional de la Plata. Facultad de Ciencias Médicas. Instituto de Investigaciones Bioquímicas de La Plata "Prof. Dr. Rodolfo R. Brenner"; ArgentinaElsevier Ireland2011-06info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/78831Crespo, Rosana; Villaverde, Maria Luciana; Girotti, Juan Roberto; Güerci, Alba Mabel; Juarez, Marta Patricia; et al.; Cytotoxic and genotoxic effects of defence secretion of Ulomoides dermestoides on A549 cells; Elsevier Ireland; Journal of Ethnopharmacology; 136; 1; 6-2011; 204-2090378-8741CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1016/j.jep.2011.04.056info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0378874111003084info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2024-05-08T13:38:02Zoai:ri.conicet.gov.ar:11336/78831instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982024-05-08 13:38:02.427CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
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