Effects of fructose-induced metabolic syndrome on rat skeletal cells and tissue, and their responses to metformin treatment

Aims: Deleterious effects of metabolic syndrome (MS) on bone are still controversial. In this study we evaluated the effects of a fructose-induced MS, and/or an oral treatment with metformin on the osteogenic potential of bone marrow mesenchymal stromal cells (MSC), as well as on bone formation and...

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Autores: Felice, Juan Ignacio, Schurman, León, McCarthy, Antonio Desmond, Sedlinsky, Claudia, Aguirre, José Ignacio, Cortizo, Ana María
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2017
País:Argentina
Institución:Comisión de Investigaciones Científicas de la Provincia de Buenos Aires
Repositorio:CIC Digital (CICBA)
Idioma:inglés
OAI Identifier:oai:digital.cic.gba.gob.ar:11746/11325
Acceso en línea:https://digital.cic.gba.gob.ar/handle/11746/11325
Access Level:acceso abierto
Palabra clave:Ciencias Biológicas
Metabolic syndrome
Fructose
Bone architecture
Osteoblasts
Metformin
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spelling Effects of fructose-induced metabolic syndrome on rat skeletal cells and tissue, and their responses to metformin treatmentFelice, Juan IgnacioSchurman, LeónMcCarthy, Antonio DesmondSedlinsky, ClaudiaAguirre, José IgnacioCortizo, Ana MaríaCiencias BiológicasMetabolic syndromeFructoseBone architectureOsteoblastsMetforminAims: Deleterious effects of metabolic syndrome (MS) on bone are still controversial. In this study we evaluated the effects of a fructose-induced MS, and/or an oral treatment with metformin on the osteogenic potential of bone marrow mesenchymal stromal cells (MSC), as well as on bone formation and architecture. Methods: 32 male 8 week-old Wistar rats were assigned to four groups: control (C), control plus oral metformin (CM), rats receiving 10% fructose in drinking water (FRD), and FRD plus metformin (FRDM). Samples were collected to measure blood parameters, and to perform pQCT analysis and static and dynamic histomorphometry. MSC were isolated to determine their osteogenic potential. Results: Metformin improved blood parameters in FRDM rats. pQCTand static and dynamic histomorphometry showed no significant differences in trabecular and cortical bone parameters among groups. FRD reduced TRAP expression and osteocyte density in trabecular bone and metformin only normalized osteocyte density. FRD decreased the osteogenic potential of MSC and metformin administration could revert some of these parameters. Conclusions: FRD-induced MS shows reduction in MSC osteogenic potential, in osteocyte density and in TRAP activity. Oral metformin treatment was able to prevent trabecular osteocyte loss and the reduction in extracellular mineralization induced by FRD-induced MS.2017info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttps://digital.cic.gba.gob.ar/handle/11746/11325enginfo:eu-repo/semantics/altIdentifier/doi/10.1016/j.diabres.2017.02.011info:eu-repo/semantics/altIdentifier/issn/0168-8227info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/reponame:CIC Digital (CICBA)instname:Comisión de Investigaciones Científicas de la Provincia de Buenos Airesinstacron:CICBA2024-05-10T10:39:27Zoai:digital.cic.gba.gob.ar:11746/11325Institucionalhttp://digital.cic.gba.gob.arOrganismo científico-tecnológicoNo correspondehttp://digital.cic.gba.gob.ar/oai/snrdmarisa.degiusti@sedici.unlp.edu.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:94412024-05-10 10:39:28.028CIC Digital (CICBA) - Comisión de Investigaciones Científicas de la Provincia de Buenos Airesfalse
dc.title.none.fl_str_mv Effects of fructose-induced metabolic syndrome on rat skeletal cells and tissue, and their responses to metformin treatment
title Effects of fructose-induced metabolic syndrome on rat skeletal cells and tissue, and their responses to metformin treatment
spellingShingle Effects of fructose-induced metabolic syndrome on rat skeletal cells and tissue, and their responses to metformin treatment
Felice, Juan Ignacio
Ciencias Biológicas
Metabolic syndrome
Fructose
Bone architecture
Osteoblasts
Metformin
title_short Effects of fructose-induced metabolic syndrome on rat skeletal cells and tissue, and their responses to metformin treatment
title_full Effects of fructose-induced metabolic syndrome on rat skeletal cells and tissue, and their responses to metformin treatment
title_fullStr Effects of fructose-induced metabolic syndrome on rat skeletal cells and tissue, and their responses to metformin treatment
title_full_unstemmed Effects of fructose-induced metabolic syndrome on rat skeletal cells and tissue, and their responses to metformin treatment
title_sort Effects of fructose-induced metabolic syndrome on rat skeletal cells and tissue, and their responses to metformin treatment
dc.creator.none.fl_str_mv Felice, Juan Ignacio
Schurman, León
McCarthy, Antonio Desmond
Sedlinsky, Claudia
Aguirre, José Ignacio
Cortizo, Ana María
author Felice, Juan Ignacio
author_facet Felice, Juan Ignacio
Schurman, León
McCarthy, Antonio Desmond
Sedlinsky, Claudia
Aguirre, José Ignacio
Cortizo, Ana María
author_role author
author2 Schurman, León
McCarthy, Antonio Desmond
Sedlinsky, Claudia
Aguirre, José Ignacio
Cortizo, Ana María
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv Ciencias Biológicas
Metabolic syndrome
Fructose
Bone architecture
Osteoblasts
Metformin
topic Ciencias Biológicas
Metabolic syndrome
Fructose
Bone architecture
Osteoblasts
Metformin
description Aims: Deleterious effects of metabolic syndrome (MS) on bone are still controversial. In this study we evaluated the effects of a fructose-induced MS, and/or an oral treatment with metformin on the osteogenic potential of bone marrow mesenchymal stromal cells (MSC), as well as on bone formation and architecture. Methods: 32 male 8 week-old Wistar rats were assigned to four groups: control (C), control plus oral metformin (CM), rats receiving 10% fructose in drinking water (FRD), and FRD plus metformin (FRDM). Samples were collected to measure blood parameters, and to perform pQCT analysis and static and dynamic histomorphometry. MSC were isolated to determine their osteogenic potential. Results: Metformin improved blood parameters in FRDM rats. pQCTand static and dynamic histomorphometry showed no significant differences in trabecular and cortical bone parameters among groups. FRD reduced TRAP expression and osteocyte density in trabecular bone and metformin only normalized osteocyte density. FRD decreased the osteogenic potential of MSC and metformin administration could revert some of these parameters. Conclusions: FRD-induced MS shows reduction in MSC osteogenic potential, in osteocyte density and in TRAP activity. Oral metformin treatment was able to prevent trabecular osteocyte loss and the reduction in extracellular mineralization induced by FRD-induced MS.
publishDate 2017
dc.date.none.fl_str_mv 2017
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://digital.cic.gba.gob.ar/handle/11746/11325
url https://digital.cic.gba.gob.ar/handle/11746/11325
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1016/j.diabres.2017.02.011
info:eu-repo/semantics/altIdentifier/issn/0168-8227
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by-nc-sa/4.0/
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-sa/4.0/
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv reponame:CIC Digital (CICBA)
instname:Comisión de Investigaciones Científicas de la Provincia de Buenos Aires
instacron:CICBA
instname_str Comisión de Investigaciones Científicas de la Provincia de Buenos Aires
instacron_str CICBA
institution CICBA
reponame_str CIC Digital (CICBA)
collection CIC Digital (CICBA)
repository.name.fl_str_mv CIC Digital (CICBA) - Comisión de Investigaciones Científicas de la Provincia de Buenos Aires
repository.mail.fl_str_mv marisa.degiusti@sedici.unlp.edu.ar
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