Proteomic Analysis Identifies Molecular Players and Biological Processes Specific to SARS-CoV-2 Exposure in Endothelial Cells

Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has been responsible for the severe pandemic of acute respiratory disease, coronavirus disease 2019 (COVID-19), experienced in the 21st century. The clinical manifestations range from mild symptoms to abnormal blood coagulation and severe...

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Autores: Corrêa De Melo, Thatiana, Trevisan Silva, Dilza, Alvarez Flores, Miryam P., Nascimento Gomes, Renata, Medina De Souza, Marcelo, Valerio, Hellen Paula, Oliveira, Douglas S., DeOcesano Pereira, Carlos, Fongaro Botosso, Viviane, Calil Jorge, Soraia Attie, Schattner, Mirta, Gómez, Ricardo Martín, Chudzinski Tavassi, Ana Marisa
Formato: artículo
Estado:Versión publicada
Fecha de publicación:2022
País:Argentina
Recursos:Universidad Nacional de La Plata
Repositorio:SEDICI (UNLP)
Idioma:inglés
OAI Identifier:oai:sedici.unlp.edu.ar:10915/156970
Acesso em linha:http://sedici.unlp.edu.ar/handle/10915/156970
Access Level:acceso abierto
Palavra-chave:Ciencias Exactas
Biología
SARS-CoV-2
endothelial cells
proteomics
mass spectrometry
HUVECs
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spelling Proteomic Analysis Identifies Molecular Players and Biological Processes Specific to SARS-CoV-2 Exposure in Endothelial CellsCorrêa De Melo, ThatianaTrevisan Silva, DilzaAlvarez Flores, Miryam P.Nascimento Gomes, RenataMedina De Souza, MarceloValerio, Hellen PaulaOliveira, Douglas S.DeOcesano Pereira, CarlosFongaro Botosso, VivianeCalil Jorge, Soraia AttieSchattner, MirtaGómez, Ricardo MartínChudzinski Tavassi, Ana MarisaCiencias ExactasBiologíaSARS-CoV-2endothelial cellsproteomicsmass spectrometryHUVECsSevere acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has been responsible for the severe pandemic of acute respiratory disease, coronavirus disease 2019 (COVID-19), experienced in the 21st century. The clinical manifestations range from mild symptoms to abnormal blood coagulation and severe respiratory failure. In severe cases, COVID-19 manifests as a thromboinflammatory disease. Damage to the vascular compartment caused by SARS-CoV-2 has been linked to thrombosis, triggered by an enhanced immune response. The molecular mechanisms underlying endothelial activation have not been fully elucidated. We aimed to identify the proteins correlated to the molecular response of human umbilical vein endothelial cells (HUVECs) after exposure to SARS-CoV-2, which might help to unravel the molecular mechanisms of endothelium activation in COVID-19. In this direction, we exposed HUVECs to SARS-CoV-2 and analyzed the expression of specific cellular receptors, and changes in the proteome of HUVECs at different time points. We identified that HUVECs exhibit non-productive infection without cytopathic effects, in addition to the lack of expression of specific cell receptors known to be essential for SARS-CoV-2 entry into cells. We highlighted the enrichment of the protein SUMOylation pathway and the increase in SUMO2, which was confirmed by orthogonal assays. In conclusion, proteomic analysis revealed that the exposure to SARS-CoV-2 induced oxidative stress and changes in protein abundance and pathways enrichment that resembled endothelial dysfunction.Instituto de Biotecnología y Biología Molecular2022-09info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionArticulohttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://sedici.unlp.edu.ar/handle/10915/156970enginfo:eu-repo/semantics/altIdentifier/issn/1422-0067info:eu-repo/semantics/altIdentifier/doi/10.3390/ijms231810452info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by/4.0/Creative Commons Attribution 4.0 International (CC BY 4.0)reponame:SEDICI (UNLP)instname:Universidad Nacional de La Platainstacron:UNLP2024-05-08T13:19:36Zoai:sedici.unlp.edu.ar:10915/156970Institucionalhttp://sedici.unlp.edu.ar/Universidad públicaNo correspondehttp://sedici.unlp.edu.ar/oai/snrdalira@sedici.unlp.edu.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:13292024-05-08 13:19:36.173SEDICI (UNLP) - Universidad Nacional de La Platafalse
dc.title.none.fl_str_mv Proteomic Analysis Identifies Molecular Players and Biological Processes Specific to SARS-CoV-2 Exposure in Endothelial Cells
title Proteomic Analysis Identifies Molecular Players and Biological Processes Specific to SARS-CoV-2 Exposure in Endothelial Cells
spellingShingle Proteomic Analysis Identifies Molecular Players and Biological Processes Specific to SARS-CoV-2 Exposure in Endothelial Cells
Corrêa De Melo, Thatiana
Ciencias Exactas
Biología
SARS-CoV-2
endothelial cells
proteomics
mass spectrometry
HUVECs
title_short Proteomic Analysis Identifies Molecular Players and Biological Processes Specific to SARS-CoV-2 Exposure in Endothelial Cells
title_full Proteomic Analysis Identifies Molecular Players and Biological Processes Specific to SARS-CoV-2 Exposure in Endothelial Cells
title_fullStr Proteomic Analysis Identifies Molecular Players and Biological Processes Specific to SARS-CoV-2 Exposure in Endothelial Cells
title_full_unstemmed Proteomic Analysis Identifies Molecular Players and Biological Processes Specific to SARS-CoV-2 Exposure in Endothelial Cells
title_sort Proteomic Analysis Identifies Molecular Players and Biological Processes Specific to SARS-CoV-2 Exposure in Endothelial Cells
dc.creator.none.fl_str_mv Corrêa De Melo, Thatiana
Trevisan Silva, Dilza
Alvarez Flores, Miryam P.
Nascimento Gomes, Renata
Medina De Souza, Marcelo
Valerio, Hellen Paula
Oliveira, Douglas S.
DeOcesano Pereira, Carlos
Fongaro Botosso, Viviane
Calil Jorge, Soraia Attie
Schattner, Mirta
Gómez, Ricardo Martín
Chudzinski Tavassi, Ana Marisa
author Corrêa De Melo, Thatiana
author_facet Corrêa De Melo, Thatiana
Trevisan Silva, Dilza
Alvarez Flores, Miryam P.
Nascimento Gomes, Renata
Medina De Souza, Marcelo
Valerio, Hellen Paula
Oliveira, Douglas S.
DeOcesano Pereira, Carlos
Fongaro Botosso, Viviane
Calil Jorge, Soraia Attie
Schattner, Mirta
Gómez, Ricardo Martín
Chudzinski Tavassi, Ana Marisa
author_role author
author2 Trevisan Silva, Dilza
Alvarez Flores, Miryam P.
Nascimento Gomes, Renata
Medina De Souza, Marcelo
Valerio, Hellen Paula
Oliveira, Douglas S.
DeOcesano Pereira, Carlos
Fongaro Botosso, Viviane
Calil Jorge, Soraia Attie
Schattner, Mirta
Gómez, Ricardo Martín
Chudzinski Tavassi, Ana Marisa
author2_role author
author
author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Ciencias Exactas
Biología
SARS-CoV-2
endothelial cells
proteomics
mass spectrometry
HUVECs
topic Ciencias Exactas
Biología
SARS-CoV-2
endothelial cells
proteomics
mass spectrometry
HUVECs
description Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has been responsible for the severe pandemic of acute respiratory disease, coronavirus disease 2019 (COVID-19), experienced in the 21st century. The clinical manifestations range from mild symptoms to abnormal blood coagulation and severe respiratory failure. In severe cases, COVID-19 manifests as a thromboinflammatory disease. Damage to the vascular compartment caused by SARS-CoV-2 has been linked to thrombosis, triggered by an enhanced immune response. The molecular mechanisms underlying endothelial activation have not been fully elucidated. We aimed to identify the proteins correlated to the molecular response of human umbilical vein endothelial cells (HUVECs) after exposure to SARS-CoV-2, which might help to unravel the molecular mechanisms of endothelium activation in COVID-19. In this direction, we exposed HUVECs to SARS-CoV-2 and analyzed the expression of specific cellular receptors, and changes in the proteome of HUVECs at different time points. We identified that HUVECs exhibit non-productive infection without cytopathic effects, in addition to the lack of expression of specific cell receptors known to be essential for SARS-CoV-2 entry into cells. We highlighted the enrichment of the protein SUMOylation pathway and the increase in SUMO2, which was confirmed by orthogonal assays. In conclusion, proteomic analysis revealed that the exposure to SARS-CoV-2 induced oxidative stress and changes in protein abundance and pathways enrichment that resembled endothelial dysfunction.
publishDate 2022
dc.date.none.fl_str_mv 2022-09
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Articulo
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://sedici.unlp.edu.ar/handle/10915/156970
url http://sedici.unlp.edu.ar/handle/10915/156970
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/issn/1422-0067
info:eu-repo/semantics/altIdentifier/doi/10.3390/ijms231810452
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by/4.0/
Creative Commons Attribution 4.0 International (CC BY 4.0)
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by/4.0/
Creative Commons Attribution 4.0 International (CC BY 4.0)
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv reponame:SEDICI (UNLP)
instname:Universidad Nacional de La Plata
instacron:UNLP
instname_str Universidad Nacional de La Plata
instacron_str UNLP
institution UNLP
reponame_str SEDICI (UNLP)
collection SEDICI (UNLP)
repository.name.fl_str_mv SEDICI (UNLP) - Universidad Nacional de La Plata
repository.mail.fl_str_mv alira@sedici.unlp.edu.ar
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