Secretome profile of Cellulomonas sp. B6 growing on lignocellulosic substrates

Aims: Lignocellulosic biomass deconstruction is a bottleneck for obtaining biofuels and value-added products. Our main goal was to characterize the secretome of a novel isolate, Cellulomonas sp. B6, when grown on residual biomass for the formulation of cost-efficient enzymatic cocktails. Methods and...

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Authors: Piccinni, Florencia Elizabeth, Ontañon, Ornella Mailén, Ghio, Silvina, Sauka, Diego Herman, Talia, Paola Mónica, Rivarola, Máximo Lisandro, Valacco, María Pia, Campos, Eleonora
Format: article
Status:Published version
Publication Date:2019
Country:Argentina
Institution:Consejo Nacional de Investigaciones Científicas y Técnicas
Repository:CONICET Digital (CONICET)
Language:English
OAI Identifier:oai:ri.conicet.gov.ar:11336/123266
Online Access:http://hdl.handle.net/11336/123266
Access Level:Open access
Keyword:CAZYMES
CELLULOMONAS
GH10
GLUCANASES
GLYCOSYL HYDROLASES
LIGNOCELLULOSE
SECRETOME
XYLANASES
https://purl.org/becyt/ford/4.1
https://purl.org/becyt/ford/4
id AR_4a11c135b2cb4f4cb71f3c562b95e7ea
oai_identifier_str oai:ri.conicet.gov.ar:11336/123266
network_acronym_str AR
network_name_str Argentina
repository_id_str
dc.title.none.fl_str_mv Secretome profile of Cellulomonas sp. B6 growing on lignocellulosic substrates
title Secretome profile of Cellulomonas sp. B6 growing on lignocellulosic substrates
spellingShingle Secretome profile of Cellulomonas sp. B6 growing on lignocellulosic substrates
Piccinni, Florencia Elizabeth
CAZYMES
CELLULOMONAS
GH10
GLUCANASES
GLYCOSYL HYDROLASES
LIGNOCELLULOSE
SECRETOME
XYLANASES
https://purl.org/becyt/ford/4.1
https://purl.org/becyt/ford/4
title_short Secretome profile of Cellulomonas sp. B6 growing on lignocellulosic substrates
title_full Secretome profile of Cellulomonas sp. B6 growing on lignocellulosic substrates
title_fullStr Secretome profile of Cellulomonas sp. B6 growing on lignocellulosic substrates
title_full_unstemmed Secretome profile of Cellulomonas sp. B6 growing on lignocellulosic substrates
title_sort Secretome profile of Cellulomonas sp. B6 growing on lignocellulosic substrates
dc.creator.none.fl_str_mv Piccinni, Florencia Elizabeth
Ontañon, Ornella Mailén
Ghio, Silvina
Sauka, Diego Herman
Talia, Paola Mónica
Rivarola, Máximo Lisandro
Valacco, María Pia
Campos, Eleonora
author Piccinni, Florencia Elizabeth
author_facet Piccinni, Florencia Elizabeth
Ontañon, Ornella Mailén
Ghio, Silvina
Sauka, Diego Herman
Talia, Paola Mónica
Rivarola, Máximo Lisandro
Valacco, María Pia
Campos, Eleonora
author_role author
author2 Ontañon, Ornella Mailén
Ghio, Silvina
Sauka, Diego Herman
Talia, Paola Mónica
Rivarola, Máximo Lisandro
Valacco, María Pia
Campos, Eleonora
author2_role author
author
author
author
author
author
author
dc.subject.none.fl_str_mv CAZYMES
CELLULOMONAS
GH10
GLUCANASES
GLYCOSYL HYDROLASES
LIGNOCELLULOSE
SECRETOME
XYLANASES
https://purl.org/becyt/ford/4.1
https://purl.org/becyt/ford/4
topic CAZYMES
CELLULOMONAS
GH10
GLUCANASES
GLYCOSYL HYDROLASES
LIGNOCELLULOSE
SECRETOME
XYLANASES
https://purl.org/becyt/ford/4.1
https://purl.org/becyt/ford/4
description Aims: Lignocellulosic biomass deconstruction is a bottleneck for obtaining biofuels and value-added products. Our main goal was to characterize the secretome of a novel isolate, Cellulomonas sp. B6, when grown on residual biomass for the formulation of cost-efficient enzymatic cocktails. Methods and Results: We identified 205 potential CAZymes in the genome of Cellulomonas sp. B6, 91 of which were glycoside hydrolases (GH). By secretome analysis of supernatants from cultures in either extruded wheat straw (EWS), grinded sugar cane straw (SCR) or carboxymethylcellulose (CMC), we identified which proteins played a role in lignocellulose deconstruction. Growth on CMC resulted in the secretion of two exoglucanases (GH6 and GH48) and two GH10 xylanases, while growth on SCR or EWS resulted in the identification of a diversity of CAZymes. From the 32 GHs predicted to be secreted, 22 were identified in supernatants from EWS and/or SCR cultures, including endo- and exoglucanases, xylanases, a xyloglucanase, an arabinofuranosidase/β-xylosidase, a β-glucosidase and an AA10. Surprisingly, among the xylanases, seven were GH10. Conclusions: Growth of Cellulomonas sp. B6 on lignocellulosic biomass induced the secretion of a diverse repertoire of CAZymes. Significance and Impact of the Study: Cellulomonas sp. B6 could serve as a source of lignocellulose-degrading enzymes applicable to bioprocessing and biotechnological industries.
publishDate 2019
dc.date.none.fl_str_mv 2019-03
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/123266
Piccinni, Florencia Elizabeth; Ontañon, Ornella Mailén; Ghio, Silvina; Sauka, Diego Herman; Talia, Paola Mónica; et al.; Secretome profile of Cellulomonas sp. B6 growing on lignocellulosic substrates; Wiley Blackwell Publishing, Inc; Journal of Applied Microbiology; 126; 3; 3-2019; 811-825
1364-5072
CONICET Digital
CONICET
url http://hdl.handle.net/11336/123266
identifier_str_mv Piccinni, Florencia Elizabeth; Ontañon, Ornella Mailén; Ghio, Silvina; Sauka, Diego Herman; Talia, Paola Mónica; et al.; Secretome profile of Cellulomonas sp. B6 growing on lignocellulosic substrates; Wiley Blackwell Publishing, Inc; Journal of Applied Microbiology; 126; 3; 3-2019; 811-825
1364-5072
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://onlinelibrary.wiley.com/doi/abs/10.1111/jam.14176
info:eu-repo/semantics/altIdentifier/doi/doi:10.1111/jam.14176
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Wiley Blackwell Publishing, Inc
publisher.none.fl_str_mv Wiley Blackwell Publishing, Inc
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1799195312971055104
spelling Secretome profile of Cellulomonas sp. B6 growing on lignocellulosic substratesPiccinni, Florencia ElizabethOntañon, Ornella MailénGhio, SilvinaSauka, Diego HermanTalia, Paola MónicaRivarola, Máximo LisandroValacco, María PiaCampos, EleonoraCAZYMESCELLULOMONASGH10GLUCANASESGLYCOSYL HYDROLASESLIGNOCELLULOSESECRETOMEXYLANASEShttps://purl.org/becyt/ford/4.1https://purl.org/becyt/ford/4Aims: Lignocellulosic biomass deconstruction is a bottleneck for obtaining biofuels and value-added products. Our main goal was to characterize the secretome of a novel isolate, Cellulomonas sp. B6, when grown on residual biomass for the formulation of cost-efficient enzymatic cocktails. Methods and Results: We identified 205 potential CAZymes in the genome of Cellulomonas sp. B6, 91 of which were glycoside hydrolases (GH). By secretome analysis of supernatants from cultures in either extruded wheat straw (EWS), grinded sugar cane straw (SCR) or carboxymethylcellulose (CMC), we identified which proteins played a role in lignocellulose deconstruction. Growth on CMC resulted in the secretion of two exoglucanases (GH6 and GH48) and two GH10 xylanases, while growth on SCR or EWS resulted in the identification of a diversity of CAZymes. From the 32 GHs predicted to be secreted, 22 were identified in supernatants from EWS and/or SCR cultures, including endo- and exoglucanases, xylanases, a xyloglucanase, an arabinofuranosidase/β-xylosidase, a β-glucosidase and an AA10. Surprisingly, among the xylanases, seven were GH10. Conclusions: Growth of Cellulomonas sp. B6 on lignocellulosic biomass induced the secretion of a diverse repertoire of CAZymes. Significance and Impact of the Study: Cellulomonas sp. B6 could serve as a source of lignocellulose-degrading enzymes applicable to bioprocessing and biotechnological industries.Fil: Piccinni, Florencia Elizabeth. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación En Ciencias Veterinarias y Agronómicas. Instituto de Agrobiotecnología y Biología Molecular. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Agrobiotecnología y Biología Molecular; ArgentinaFil: Ontañon, Ornella Mailén. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación En Ciencias Veterinarias y Agronómicas. Instituto de Agrobiotecnología y Biología Molecular. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Agrobiotecnología y Biología Molecular; ArgentinaFil: Ghio, Silvina. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación En Ciencias Veterinarias y Agronómicas. Instituto de Agrobiotecnología y Biología Molecular. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Agrobiotecnología y Biología Molecular; ArgentinaFil: Sauka, Diego Herman. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación En Ciencias Veterinarias y Agronómicas. Instituto de Agrobiotecnología y Biología Molecular. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Agrobiotecnología y Biología Molecular; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Microbiología y Zoología Agrícola; ArgentinaFil: Talia, Paola Mónica. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación En Ciencias Veterinarias y Agronómicas. Instituto de Agrobiotecnología y Biología Molecular. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Agrobiotecnología y Biología Molecular; ArgentinaFil: Rivarola, Máximo Lisandro. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación En Ciencias Veterinarias y Agronómicas. Instituto de Agrobiotecnología y Biología Molecular. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Agrobiotecnología y Biología Molecular; ArgentinaFil: Valacco, María Pia. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales; ArgentinaFil: Campos, Eleonora. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación En Ciencias Veterinarias y Agronómicas. Instituto de Agrobiotecnología y Biología Molecular. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Agrobiotecnología y Biología Molecular; ArgentinaWiley Blackwell Publishing, Inc2019-03info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/123266Piccinni, Florencia Elizabeth; Ontañon, Ornella Mailén; Ghio, Silvina; Sauka, Diego Herman; Talia, Paola Mónica; et al.; Secretome profile of Cellulomonas sp. B6 growing on lignocellulosic substrates; Wiley Blackwell Publishing, Inc; Journal of Applied Microbiology; 126; 3; 3-2019; 811-8251364-5072CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://onlinelibrary.wiley.com/doi/abs/10.1111/jam.14176info:eu-repo/semantics/altIdentifier/doi/doi:10.1111/jam.14176info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2024-05-08T13:48:48Zoai:ri.conicet.gov.ar:11336/123266instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982024-05-08 13:48:48.584CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
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