Influence of culture medium supplementation with linoleic acid on survival to cryopreservation of in vitro bovine embryos
Studies report that the addition of linoleic acid to bovine embryo production media in vitro improves its resistance to conventional freezing. In this study the effect on survival of in vitro bovine embryos under freezing was evaluated by the addition of linoleic acid (LA) in the culture medium on d...
| Autores: | , , , |
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| Tipo de recurso: | artículo |
| Estado: | Versión publicada |
| Fecha de publicación: | 2017 |
| País: | Argentina |
| Institución: | Consejo Nacional de Investigaciones Científicas y Técnicas |
| Repositorio: | CONICET Digital (CONICET) |
| Idioma: | inglés |
| OAI Identifier: | oai:ri.conicet.gov.ar:11336/181535 |
| Acceso en línea: | http://hdl.handle.net/11336/181535 |
| Access Level: | acceso abierto |
| Palabra clave: | EMBRYO LYNOLEIC ACID IVF IN VITRO https://purl.org/becyt/ford/4.4 https://purl.org/becyt/ford/4 |
| Sumario: | Studies report that the addition of linoleic acid to bovine embryo production media in vitro improves its resistance to conventional freezing. In this study the effect on survival of in vitro bovine embryos under freezing was evaluated by the addition of linoleic acid (LA) in the culture medium on days 3 or 5. A total of 1452 oocytes were recovered and assigned to 4 experimental groups and a control group. Six replicates were made to obtain approximately 100 freeze-thawed embryos per group. Group 1: 100 M on Day 3, group 2: 50 M on Day 3, group 3: 100 M on Day 5, group 4: 50 M on Day 5. The embryos were frozen and one week after they were thawed to evaluate the percentages of re-expansion (24 h) and hatching (72 h). The results were analyzed by ANOVA and when the differences were significant, the means between the groups were compared by Fischer's LSD Test (p <0.05). It was observed that the percentage of re-expansion was significantly improved with the addition of 50 μM LA on Day 5 (54.94 ± 28.8 vs. 19.19 ± 28.4, p = 0.001) and the percentage of hatching with the addition of 50 μM of LA at day 3 (37.9 ± 18.3 vs. 9.02 ± 12.0, p = 0.014), when them were compared with the control group. In conclusion, the addition of linoleic acid improves the survival to freezing of bovine embryos produced by in vitro techniques |
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