Quantification of acetylated tubulin

The acetylation/deacetylation of Lys40 of the α-subunit is an important posttranslational modification undergone by tubulin during the life of a cell. Many previous studies have addressed the physiological role of this acetylation process using various approaches based on changes of acetylated tubul...

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Autores: Chesta, María Eugenia, Carbajal, Agustin, Bisig, Carlos Gaston, Arce, Carlos Angel
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2013
País:Argentina
Institución:Consejo Nacional de Investigaciones Científicas y Técnicas
Repositorio:CONICET Digital (CONICET)
Idioma:inglés
OAI Identifier:oai:ri.conicet.gov.ar:11336/25696
Acceso en línea:http://hdl.handle.net/11336/25696
Access Level:acceso abierto
Palabra clave:Acetylated tubulin
Microtubules
Quantification method
https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
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repository_id_str
spelling Quantification of acetylated tubulinChesta, María EugeniaCarbajal, AgustinBisig, Carlos GastonArce, Carlos AngelAcetylated tubulinMicrotubulesQuantification methodhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1The acetylation/deacetylation of Lys40 of the α-subunit is an important posttranslational modification undergone by tubulin during the life of a cell. Many previous studies have addressed the physiological role of this acetylation process using various approaches based on changes of acetylated tubulin (AcTubulin) content. In most of these studies, however, the actual amounts of AcTubulin were not known and it was difficult to draw conclusions. We present here a simple method to estimate the percentage of AcTubulin relative to total tubulin. The method is based on acetylation of the tubulin sample with acetic anhydride, Western blotting stained by antiAcTubulin antibody, and comparison of the optical density of the AcTubulin band with that of a corresponding sample that was not chemically acetylated.Fil: Chesta, María Eugenia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Centro de Investigaciones en Química Biológica de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Centro de Investigaciones en Química Biológica de Córdoba; ArgentinaFil: Carbajal, Agustin. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Centro de Investigaciones en Química Biológica de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Centro de Investigaciones en Química Biológica de Córdoba; ArgentinaFil: Bisig, Carlos Gaston. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Centro de Investigaciones en Química Biológica de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Centro de Investigaciones en Química Biológica de Córdoba; ArgentinaFil: Arce, Carlos Angel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Centro de Investigaciones en Química Biológica de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Centro de Investigaciones en Química Biológica de Córdoba; ArgentinaWiley2013-05info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/25696Chesta, María Eugenia; Carbajal, Agustin; Bisig, Carlos Gaston; Arce, Carlos Angel; Quantification of acetylated tubulin; Wiley; Cytoskeleton; 70; 6; 5-2013; 297-3031949-3592CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1002/cm.21111info:eu-repo/semantics/altIdentifier/url/http://onlinelibrary.wiley.com/doi/10.1002/cm.21111/abstractinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2024-05-08T14:07:44Zoai:ri.conicet.gov.ar:11336/25696instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982024-05-08 14:07:44.402CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Quantification of acetylated tubulin
title Quantification of acetylated tubulin
spellingShingle Quantification of acetylated tubulin
Chesta, María Eugenia
Acetylated tubulin
Microtubules
Quantification method
https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
title_short Quantification of acetylated tubulin
title_full Quantification of acetylated tubulin
title_fullStr Quantification of acetylated tubulin
title_full_unstemmed Quantification of acetylated tubulin
title_sort Quantification of acetylated tubulin
dc.creator.none.fl_str_mv Chesta, María Eugenia
Carbajal, Agustin
Bisig, Carlos Gaston
Arce, Carlos Angel
author Chesta, María Eugenia
author_facet Chesta, María Eugenia
Carbajal, Agustin
Bisig, Carlos Gaston
Arce, Carlos Angel
author_role author
author2 Carbajal, Agustin
Bisig, Carlos Gaston
Arce, Carlos Angel
author2_role author
author
author
dc.subject.none.fl_str_mv Acetylated tubulin
Microtubules
Quantification method
https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
topic Acetylated tubulin
Microtubules
Quantification method
https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
description The acetylation/deacetylation of Lys40 of the α-subunit is an important posttranslational modification undergone by tubulin during the life of a cell. Many previous studies have addressed the physiological role of this acetylation process using various approaches based on changes of acetylated tubulin (AcTubulin) content. In most of these studies, however, the actual amounts of AcTubulin were not known and it was difficult to draw conclusions. We present here a simple method to estimate the percentage of AcTubulin relative to total tubulin. The method is based on acetylation of the tubulin sample with acetic anhydride, Western blotting stained by antiAcTubulin antibody, and comparison of the optical density of the AcTubulin band with that of a corresponding sample that was not chemically acetylated.
publishDate 2013
dc.date.none.fl_str_mv 2013-05
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/25696
Chesta, María Eugenia; Carbajal, Agustin; Bisig, Carlos Gaston; Arce, Carlos Angel; Quantification of acetylated tubulin; Wiley; Cytoskeleton; 70; 6; 5-2013; 297-303
1949-3592
CONICET Digital
CONICET
url http://hdl.handle.net/11336/25696
identifier_str_mv Chesta, María Eugenia; Carbajal, Agustin; Bisig, Carlos Gaston; Arce, Carlos Angel; Quantification of acetylated tubulin; Wiley; Cytoskeleton; 70; 6; 5-2013; 297-303
1949-3592
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1002/cm.21111
info:eu-repo/semantics/altIdentifier/url/http://onlinelibrary.wiley.com/doi/10.1002/cm.21111/abstract
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Wiley
publisher.none.fl_str_mv Wiley
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 15,811543

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