Exopolysaccharide biosynthesis by Lactobacillus helveticus ATCC 15807

Exopolysaccharide (EPS) production and the activities of the enzymes involved in sugar nucleotide biosynthesis in Lactobacillus helveticus ATCC 15807 under controlled pH conditions were investigated. Batch fermentations using lactose as energy source showed higher EPS synthesis by L. helveticus ATCC...

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Autores: Torino, Maria Ines, Mozzi, Fernanda Beatriz, Font, Graciela Maria
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2005
País:Argentina
Institución:Consejo Nacional de Investigaciones Científicas y Técnicas
Repositorio:CONICET Digital (CONICET)
Idioma:inglés
OAI Identifier:oai:ri.conicet.gov.ar:11336/55439
Acceso en línea:http://hdl.handle.net/11336/55439
Access Level:acceso abierto
Palabra clave:https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
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spelling Exopolysaccharide biosynthesis by Lactobacillus helveticus ATCC 15807Torino, Maria InesMozzi, Fernanda BeatrizFont, Graciela Mariahttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Exopolysaccharide (EPS) production and the activities of the enzymes involved in sugar nucleotide biosynthesis in Lactobacillus helveticus ATCC 15807 under controlled pH conditions were investigated. Batch fermentations using lactose as energy source showed higher EPS synthesis by L. helveticus ATCC 15807 at pH 4.5 with respect to pH 6.2, the enzyme α-phosphoglucomutase (α-PGM) being correlated with both total and specific EPS production. When glucose was used as carbon source instead of lactose, the lower EPS synthesis obtained was linked to a decrease in α-PGM and galactose 1-phosphate-uridyltransferase (GalT) activities, the reduction of the latter being more pronounced. Higher EPS production by L. helveticus ATCC 15807 at the acidic constant pH of 4.5 requires that both α-PGM and GalT activities are high. These enzymes are needed to synthesize UDP-glucose and UDP-galactose for supplying the corresponding monomers for EPS biosynthesis. Although differences are observed in EPS production by this strain regarding the energy source (lactose or glucose), the monomeric composition of the polymers produced is independent of the carbohydrate used. The obtained results contribute to a better understanding of the physiological factors that affect EPS biosynthesis by lactobacilli, which could help in the correct handling of the fermentation parameters within the fermented dairy industry.Fil: Torino, Maria Ines. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; ArgentinaFil: Mozzi, Fernanda Beatriz. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; ArgentinaFil: Font, Graciela Maria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; ArgentinaSpringer2005-07info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/55439Torino, Maria Ines; Mozzi, Fernanda Beatriz; Font, Graciela Maria; Exopolysaccharide biosynthesis by Lactobacillus helveticus ATCC 15807; Springer; Applied Microbiology and Biotechnology; 68; 2; 7-2005; 259-2650175-75981432-0614CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1007/s00253-004-1865-2info:eu-repo/semantics/altIdentifier/url/https://link.springer.com/article/10.1007%2Fs00253-004-1865-2info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2024-05-08T14:29:11Zoai:ri.conicet.gov.ar:11336/55439instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982024-05-08 14:29:12.119CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Exopolysaccharide biosynthesis by Lactobacillus helveticus ATCC 15807
title Exopolysaccharide biosynthesis by Lactobacillus helveticus ATCC 15807
spellingShingle Exopolysaccharide biosynthesis by Lactobacillus helveticus ATCC 15807
Torino, Maria Ines
https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
title_short Exopolysaccharide biosynthesis by Lactobacillus helveticus ATCC 15807
title_full Exopolysaccharide biosynthesis by Lactobacillus helveticus ATCC 15807
title_fullStr Exopolysaccharide biosynthesis by Lactobacillus helveticus ATCC 15807
title_full_unstemmed Exopolysaccharide biosynthesis by Lactobacillus helveticus ATCC 15807
title_sort Exopolysaccharide biosynthesis by Lactobacillus helveticus ATCC 15807
dc.creator.none.fl_str_mv Torino, Maria Ines
Mozzi, Fernanda Beatriz
Font, Graciela Maria
author Torino, Maria Ines
author_facet Torino, Maria Ines
Mozzi, Fernanda Beatriz
Font, Graciela Maria
author_role author
author2 Mozzi, Fernanda Beatriz
Font, Graciela Maria
author2_role author
author
dc.subject.none.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
topic https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
description Exopolysaccharide (EPS) production and the activities of the enzymes involved in sugar nucleotide biosynthesis in Lactobacillus helveticus ATCC 15807 under controlled pH conditions were investigated. Batch fermentations using lactose as energy source showed higher EPS synthesis by L. helveticus ATCC 15807 at pH 4.5 with respect to pH 6.2, the enzyme α-phosphoglucomutase (α-PGM) being correlated with both total and specific EPS production. When glucose was used as carbon source instead of lactose, the lower EPS synthesis obtained was linked to a decrease in α-PGM and galactose 1-phosphate-uridyltransferase (GalT) activities, the reduction of the latter being more pronounced. Higher EPS production by L. helveticus ATCC 15807 at the acidic constant pH of 4.5 requires that both α-PGM and GalT activities are high. These enzymes are needed to synthesize UDP-glucose and UDP-galactose for supplying the corresponding monomers for EPS biosynthesis. Although differences are observed in EPS production by this strain regarding the energy source (lactose or glucose), the monomeric composition of the polymers produced is independent of the carbohydrate used. The obtained results contribute to a better understanding of the physiological factors that affect EPS biosynthesis by lactobacilli, which could help in the correct handling of the fermentation parameters within the fermented dairy industry.
publishDate 2005
dc.date.none.fl_str_mv 2005-07
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/55439
Torino, Maria Ines; Mozzi, Fernanda Beatriz; Font, Graciela Maria; Exopolysaccharide biosynthesis by Lactobacillus helveticus ATCC 15807; Springer; Applied Microbiology and Biotechnology; 68; 2; 7-2005; 259-265
0175-7598
1432-0614
CONICET Digital
CONICET
url http://hdl.handle.net/11336/55439
identifier_str_mv Torino, Maria Ines; Mozzi, Fernanda Beatriz; Font, Graciela Maria; Exopolysaccharide biosynthesis by Lactobacillus helveticus ATCC 15807; Springer; Applied Microbiology and Biotechnology; 68; 2; 7-2005; 259-265
0175-7598
1432-0614
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1007/s00253-004-1865-2
info:eu-repo/semantics/altIdentifier/url/https://link.springer.com/article/10.1007%2Fs00253-004-1865-2
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Springer
publisher.none.fl_str_mv Springer
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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