Time-resolved electrochemical quantification of azanone (HNO) at low nanomolar level

Azanone (HNO, nitroxyl) is a highly reactive and short-lived compound with intriguing and highly relevant properties. It has been proposed to be a reaction intermediate in several chemical reactions and an in vivo, endogenously produced key metabolite and/or signaling molecule. In addition, its dono...

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Autores: Suarez, Sebastian A., Bikel, Damian E., Wetzler, Diana Elena, Marti, Marcelo Adrian, Doctorovich, Fabio
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2013
País:Argentina
Institución:Consejo Nacional de Investigaciones Científicas y Técnicas
Repositorio:CONICET Digital (CONICET)
Idioma:inglés
OAI Identifier:oai:ri.conicet.gov.ar:11336/7892
Acceso en línea:http://hdl.handle.net/11336/7892
Access Level:acceso abierto
Palabra clave:Azone
Sensor
Electrochmical
https://purl.org/becyt/ford/1.4
https://purl.org/becyt/ford/1
id AR_44ce8dfbf35cdbcc38b5d530e1fbf22e
oai_identifier_str oai:ri.conicet.gov.ar:11336/7892
network_acronym_str AR
network_name_str Argentina
repository_id_str
dc.title.none.fl_str_mv Time-resolved electrochemical quantification of azanone (HNO) at low nanomolar level
title Time-resolved electrochemical quantification of azanone (HNO) at low nanomolar level
spellingShingle Time-resolved electrochemical quantification of azanone (HNO) at low nanomolar level
Suarez, Sebastian A.
Azone
Sensor
Electrochmical
https://purl.org/becyt/ford/1.4
https://purl.org/becyt/ford/1
title_short Time-resolved electrochemical quantification of azanone (HNO) at low nanomolar level
title_full Time-resolved electrochemical quantification of azanone (HNO) at low nanomolar level
title_fullStr Time-resolved electrochemical quantification of azanone (HNO) at low nanomolar level
title_full_unstemmed Time-resolved electrochemical quantification of azanone (HNO) at low nanomolar level
title_sort Time-resolved electrochemical quantification of azanone (HNO) at low nanomolar level
dc.creator.none.fl_str_mv Suarez, Sebastian A.
Bikel, Damian E.
Wetzler, Diana Elena
Marti, Marcelo Adrian
Doctorovich, Fabio
author Suarez, Sebastian A.
author_facet Suarez, Sebastian A.
Bikel, Damian E.
Wetzler, Diana Elena
Marti, Marcelo Adrian
Doctorovich, Fabio
author_role author
author2 Bikel, Damian E.
Wetzler, Diana Elena
Marti, Marcelo Adrian
Doctorovich, Fabio
author2_role author
author
author
author
dc.subject.none.fl_str_mv Azone
Sensor
Electrochmical
https://purl.org/becyt/ford/1.4
https://purl.org/becyt/ford/1
topic Azone
Sensor
Electrochmical
https://purl.org/becyt/ford/1.4
https://purl.org/becyt/ford/1
description Azanone (HNO, nitroxyl) is a highly reactive and short-lived compound with intriguing and highly relevant properties. It has been proposed to be a reaction intermediate in several chemical reactions and an in vivo, endogenously produced key metabolite and/or signaling molecule. In addition, its donors have important pharmacological properties. Therefore, given its relevance and elusive nature (it reacts with itself very quickly), the development of reliable analytical methods for quantitative HNO detection is in high demand for the advancement of future research in this area. During the past few years, several methods were developed that rely on chemical reactions followed by mass spectrometry, high-performance liquid chromatography, UV-vis, or fluorescence-trapping-based methodologies. In this work, our recently developed HNO-sensing electrode, based on the covalent attachment of cobalt(II) 5,10,15,20-tetrakis[3-( p -acetylthiopropoxy)phenyl] porphyrin [Co(P)] to a gold electrode, has been thoroughly characterized in terms of sensibility, accuracy, time-resolved detection, and compatibility with complex biologically compatible media. Our results show that the Co(P) electrode: (i) allows time-resolved detection and kinetic analysis of the electrode response (the underlying HNO-producing reactions can be characterized) (ii) is able to selectively detect and reliably quantify HNO in the 1?1000 nM range, and (iii) has good biological media compatibility (including cell culture), displaying a lack of spurious signals due to the presence of O 2 , NO, and other reactive nitrogen and oxygen species. In summary, the Co(P) electrode is to our knowledge the best prospect for use in studies investigating HNO-related chemical and biological reactions.
publishDate 2013
dc.date.none.fl_str_mv 2013-08-19
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/7892
Suarez, Sebastian A.; Bikel, Damian E.; Wetzler, Diana Elena; Marti, Marcelo Adrian; Doctorovich, Fabio; Time-resolved electrochemical quantification of azanone (HNO) at low nanomolar level; American Chemical Society; Analytical Chemistry; 85; 21; 19-8-2013; 10262-10269
0003-2700
url http://hdl.handle.net/11336/7892
identifier_str_mv Suarez, Sebastian A.; Bikel, Damian E.; Wetzler, Diana Elena; Marti, Marcelo Adrian; Doctorovich, Fabio; Time-resolved electrochemical quantification of azanone (HNO) at low nanomolar level; American Chemical Society; Analytical Chemistry; 85; 21; 19-8-2013; 10262-10269
0003-2700
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1021/ac402134b
info:eu-repo/semantics/altIdentifier/url/http://pubs.acs.org/doi/abs/10.1021/ac402134b
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv American Chemical Society
publisher.none.fl_str_mv American Chemical Society
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1799196386948808704
spelling Time-resolved electrochemical quantification of azanone (HNO) at low nanomolar levelSuarez, Sebastian A.Bikel, Damian E.Wetzler, Diana ElenaMarti, Marcelo AdrianDoctorovich, FabioAzoneSensorElectrochmicalhttps://purl.org/becyt/ford/1.4https://purl.org/becyt/ford/1Azanone (HNO, nitroxyl) is a highly reactive and short-lived compound with intriguing and highly relevant properties. It has been proposed to be a reaction intermediate in several chemical reactions and an in vivo, endogenously produced key metabolite and/or signaling molecule. In addition, its donors have important pharmacological properties. Therefore, given its relevance and elusive nature (it reacts with itself very quickly), the development of reliable analytical methods for quantitative HNO detection is in high demand for the advancement of future research in this area. During the past few years, several methods were developed that rely on chemical reactions followed by mass spectrometry, high-performance liquid chromatography, UV-vis, or fluorescence-trapping-based methodologies. In this work, our recently developed HNO-sensing electrode, based on the covalent attachment of cobalt(II) 5,10,15,20-tetrakis[3-( p -acetylthiopropoxy)phenyl] porphyrin [Co(P)] to a gold electrode, has been thoroughly characterized in terms of sensibility, accuracy, time-resolved detection, and compatibility with complex biologically compatible media. Our results show that the Co(P) electrode: (i) allows time-resolved detection and kinetic analysis of the electrode response (the underlying HNO-producing reactions can be characterized) (ii) is able to selectively detect and reliably quantify HNO in the 1?1000 nM range, and (iii) has good biological media compatibility (including cell culture), displaying a lack of spurious signals due to the presence of O 2 , NO, and other reactive nitrogen and oxygen species. In summary, the Co(P) electrode is to our knowledge the best prospect for use in studies investigating HNO-related chemical and biological reactions.Fil: Suarez, Sebastian A.. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Química, Física de los Materiales, Medioambiente y Energía; ArgentinaFil: Bikel, Damian E.. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Química, Física de los Materiales, Medioambiente y Energía; ArgentinaFil: Wetzler, Diana Elena. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales; Argentina. Universidad de Buenos Aires; ArgentinaFil: Marti, Marcelo Adrian. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales; ArgentinaFil: Doctorovich, Fabio. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Química, Física de los Materiales, Medioambiente y Energía; ArgentinaAmerican Chemical Society2013-08-19info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/7892Suarez, Sebastian A.; Bikel, Damian E.; Wetzler, Diana Elena; Marti, Marcelo Adrian; Doctorovich, Fabio; Time-resolved electrochemical quantification of azanone (HNO) at low nanomolar level; American Chemical Society; Analytical Chemistry; 85; 21; 19-8-2013; 10262-102690003-2700enginfo:eu-repo/semantics/altIdentifier/doi/10.1021/ac402134binfo:eu-repo/semantics/altIdentifier/url/http://pubs.acs.org/doi/abs/10.1021/ac402134binfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2024-05-08T14:25:45Zoai:ri.conicet.gov.ar:11336/7892instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982024-05-08 14:25:45.851CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
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