Morphological, molecular, and toxin analysis of field populations of Alexandrium genus from the Argentine Sea

In the Argentine Sea, blooms of toxigenic dinoflagellates of the Alexandrium tamarense species complex have led to fish and bird mortalities and human deaths as a consequence of paralytic shellfish poisoning (PSP). Yet little is known about the occurrence of other toxigenic species of the genus Alex...

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Autores: Fabro Cerreia Fus, Elena Ines, Almandoz, Gaston Osvaldo, Ferrario, Martha Elba, John, Uwe, Tillmann, Urban, Toebe, Kerstin, Krock, Bernd, Cembella, Allan
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2017
País:Argentina
Institución:Consejo Nacional de Investigaciones Científicas y Técnicas
Repositorio:CONICET Digital (CONICET)
Idioma:inglés
OAI Identifier:oai:ri.conicet.gov.ar:11336/49791
Acceso en línea:http://hdl.handle.net/11336/49791
Access Level:acceso abierto
Palabra clave:Alexandrium Minutum
Alexandrium Ostenfeldii
Lc-Fd
Lc-Ms/Ms
Pcr
Psp
South-Western Atlantic
Spirolides
https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
id AR_4415971cbcb6eeef129d5259d9126062
oai_identifier_str oai:ri.conicet.gov.ar:11336/49791
network_acronym_str AR
network_name_str Argentina
repository_id_str
dc.title.none.fl_str_mv Morphological, molecular, and toxin analysis of field populations of Alexandrium genus from the Argentine Sea
title Morphological, molecular, and toxin analysis of field populations of Alexandrium genus from the Argentine Sea
spellingShingle Morphological, molecular, and toxin analysis of field populations of Alexandrium genus from the Argentine Sea
Fabro Cerreia Fus, Elena Ines
Alexandrium Minutum
Alexandrium Ostenfeldii
Lc-Fd
Lc-Ms/Ms
Pcr
Psp
South-Western Atlantic
Spirolides
https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
title_short Morphological, molecular, and toxin analysis of field populations of Alexandrium genus from the Argentine Sea
title_full Morphological, molecular, and toxin analysis of field populations of Alexandrium genus from the Argentine Sea
title_fullStr Morphological, molecular, and toxin analysis of field populations of Alexandrium genus from the Argentine Sea
title_full_unstemmed Morphological, molecular, and toxin analysis of field populations of Alexandrium genus from the Argentine Sea
title_sort Morphological, molecular, and toxin analysis of field populations of Alexandrium genus from the Argentine Sea
dc.creator.none.fl_str_mv Fabro Cerreia Fus, Elena Ines
Almandoz, Gaston Osvaldo
Ferrario, Martha Elba
John, Uwe
Tillmann, Urban
Toebe, Kerstin
Krock, Bernd
Cembella, Allan
author Fabro Cerreia Fus, Elena Ines
author_facet Fabro Cerreia Fus, Elena Ines
Almandoz, Gaston Osvaldo
Ferrario, Martha Elba
John, Uwe
Tillmann, Urban
Toebe, Kerstin
Krock, Bernd
Cembella, Allan
author_role author
author2 Almandoz, Gaston Osvaldo
Ferrario, Martha Elba
John, Uwe
Tillmann, Urban
Toebe, Kerstin
Krock, Bernd
Cembella, Allan
author2_role author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Alexandrium Minutum
Alexandrium Ostenfeldii
Lc-Fd
Lc-Ms/Ms
Pcr
Psp
South-Western Atlantic
Spirolides
https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
topic Alexandrium Minutum
Alexandrium Ostenfeldii
Lc-Fd
Lc-Ms/Ms
Pcr
Psp
South-Western Atlantic
Spirolides
https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
description In the Argentine Sea, blooms of toxigenic dinoflagellates of the Alexandrium tamarense species complex have led to fish and bird mortalities and human deaths as a consequence of paralytic shellfish poisoning (PSP). Yet little is known about the occurrence of other toxigenic species of the genus Alexandrium, or of their toxin composition beyond coastal waters. The distribution of Alexandrium species and related toxins in the Argentine Sea was determined by sampling surface waters on an oceanographic expedition during austral spring from ~39°S to 48°S. Light microscope and SEM analysis for species identification and enumeration was supplemented by confirmatory PCR analysis from field samples. The most frequent Alexandrium taxon identified by microscopy corresponded to the classical description of A. tamarense. Only weak signals of Group I from the A. tamarense species complex were detected by PCR of bulk field samples, but phylogenetic reconstruction of rDNA sequences from single cells from one station assigned them to ribotype Group I (Alexandrium catenella). PCR probes for Alexandrium minutum and Alexandrium ostenfeldii yielded a positive signal, although A. minutum morphology did not completely match the classical description. Analysis of PSP toxin composition of plankton samples revealed toxin profiles dominated by gonyautoxins (GTX1/4). The main toxic cyclic imine detected was 13‐desMe‐spirolide C and this supported the association with A. ostenfeldii in the field. This study represents the first integrated molecular, morphological and toxinological analysis of field populations of the genus Alexandrium in the Argentine Sea.
publishDate 2017
dc.date.none.fl_str_mv 2017-12
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/49791
Fabro Cerreia Fus, Elena Ines; Almandoz, Gaston Osvaldo; Ferrario, Martha Elba; John, Uwe; Tillmann, Urban; et al.; Morphological, molecular, and toxin analysis of field populations of Alexandrium genus from the Argentine Sea; Wiley Blackwell Publishing, Inc; Journal Of Phycology; 53; 6; 12-2017; 1206-1222
0022-3646
CONICET Digital
CONICET
url http://hdl.handle.net/11336/49791
identifier_str_mv Fabro Cerreia Fus, Elena Ines; Almandoz, Gaston Osvaldo; Ferrario, Martha Elba; John, Uwe; Tillmann, Urban; et al.; Morphological, molecular, and toxin analysis of field populations of Alexandrium genus from the Argentine Sea; Wiley Blackwell Publishing, Inc; Journal Of Phycology; 53; 6; 12-2017; 1206-1222
0022-3646
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1111/jpy.12574
info:eu-repo/semantics/altIdentifier/url/https://onlinelibrary.wiley.com/doi/abs/10.1111/jpy.12574
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Wiley Blackwell Publishing, Inc
publisher.none.fl_str_mv Wiley Blackwell Publishing, Inc
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1799195122250809344
spelling Morphological, molecular, and toxin analysis of field populations of Alexandrium genus from the Argentine SeaFabro Cerreia Fus, Elena InesAlmandoz, Gaston OsvaldoFerrario, Martha ElbaJohn, UweTillmann, UrbanToebe, KerstinKrock, BerndCembella, AllanAlexandrium MinutumAlexandrium OstenfeldiiLc-FdLc-Ms/MsPcrPspSouth-Western AtlanticSpirolideshttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1In the Argentine Sea, blooms of toxigenic dinoflagellates of the Alexandrium tamarense species complex have led to fish and bird mortalities and human deaths as a consequence of paralytic shellfish poisoning (PSP). Yet little is known about the occurrence of other toxigenic species of the genus Alexandrium, or of their toxin composition beyond coastal waters. The distribution of Alexandrium species and related toxins in the Argentine Sea was determined by sampling surface waters on an oceanographic expedition during austral spring from ~39°S to 48°S. Light microscope and SEM analysis for species identification and enumeration was supplemented by confirmatory PCR analysis from field samples. The most frequent Alexandrium taxon identified by microscopy corresponded to the classical description of A. tamarense. Only weak signals of Group I from the A. tamarense species complex were detected by PCR of bulk field samples, but phylogenetic reconstruction of rDNA sequences from single cells from one station assigned them to ribotype Group I (Alexandrium catenella). PCR probes for Alexandrium minutum and Alexandrium ostenfeldii yielded a positive signal, although A. minutum morphology did not completely match the classical description. Analysis of PSP toxin composition of plankton samples revealed toxin profiles dominated by gonyautoxins (GTX1/4). The main toxic cyclic imine detected was 13‐desMe‐spirolide C and this supported the association with A. ostenfeldii in the field. This study represents the first integrated molecular, morphological and toxinological analysis of field populations of the genus Alexandrium in the Argentine Sea.Fil: Fabro Cerreia Fus, Elena Ines. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo. División Ficología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Almandoz, Gaston Osvaldo. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo. División Ficología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Ferrario, Martha Elba. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo. División Ficología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: John, Uwe. Alfred Wegener Institut-Helmholtz Zentrum fur Polar und Meeresforschung; AlemaniaFil: Tillmann, Urban. Alfred Wegener Institut-Helmholtz Zentrum fur Polar und Meeresforschung; AlemaniaFil: Toebe, Kerstin. Alfred Wegener Institut-Helmholtz Zentrum fur Polar und Meeresforschung; AlemaniaFil: Krock, Bernd. Alfred Wegener Institut-Helmholtz Zentrum fur Polar und Meeresforschung; AlemaniaFil: Cembella, Allan. Alfred Wegener Institut-Helmholtz Zentrum fur Polar und Meeresforschung; AlemaniaWiley Blackwell Publishing, Inc2017-12info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/49791Fabro Cerreia Fus, Elena Ines; Almandoz, Gaston Osvaldo; Ferrario, Martha Elba; John, Uwe; Tillmann, Urban; et al.; Morphological, molecular, and toxin analysis of field populations of Alexandrium genus from the Argentine Sea; Wiley Blackwell Publishing, Inc; Journal Of Phycology; 53; 6; 12-2017; 1206-12220022-3646CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1111/jpy.12574info:eu-repo/semantics/altIdentifier/url/https://onlinelibrary.wiley.com/doi/abs/10.1111/jpy.12574info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2024-05-08T13:44:00Zoai:ri.conicet.gov.ar:11336/49791instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982024-05-08 13:44:00.928CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
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