The antioxidant activity of a prenyl flavonoid alters its antifungal toxicity on Candida albicans biofilms

The antioxidant effect of 8PP, a prenylflavonoid from Dalea elegans on Candida albicans biofilms, was investigated. We previously reported that sensitive (SCa) and resistant C. albicans (RCa) biofilms were strongly inhibited by this compound, in a dose-depending manner (50 μM-100 μM), with a prooxid...

Descripción completa

Detalles Bibliográficos
Autores: Peralta, Mariana Andrea, Ortega, María Gabriela, Cabrera, Jose Luis, Paraje, María Gabriela
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2018
País:Argentina
Institución:Consejo Nacional de Investigaciones Científicas y Técnicas
Repositorio:CONICET Digital (CONICET)
Idioma:inglés
OAI Identifier:oai:ri.conicet.gov.ar:11336/86569
Acceso en línea:http://hdl.handle.net/11336/86569
Access Level:acceso abierto
Palabra clave:CANDIDA ALBICANS BIOFILMS
CONFOCAL SCANNING LASER MICROSCOPY
DALEA ELEGANS
FREE RADICALS
PRENYL FLAVONOID
PROOXIDANT/ANTIOXIDANT ACTION
https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
id AR_43fdaf88df05d6ca65ef6b48b372ccf2
oai_identifier_str oai:ri.conicet.gov.ar:11336/86569
network_acronym_str AR
network_name_str Argentina
repository_id_str
dc.title.none.fl_str_mv The antioxidant activity of a prenyl flavonoid alters its antifungal toxicity on Candida albicans biofilms
title The antioxidant activity of a prenyl flavonoid alters its antifungal toxicity on Candida albicans biofilms
spellingShingle The antioxidant activity of a prenyl flavonoid alters its antifungal toxicity on Candida albicans biofilms
Peralta, Mariana Andrea
CANDIDA ALBICANS BIOFILMS
CONFOCAL SCANNING LASER MICROSCOPY
DALEA ELEGANS
FREE RADICALS
PRENYL FLAVONOID
PROOXIDANT/ANTIOXIDANT ACTION
https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
title_short The antioxidant activity of a prenyl flavonoid alters its antifungal toxicity on Candida albicans biofilms
title_full The antioxidant activity of a prenyl flavonoid alters its antifungal toxicity on Candida albicans biofilms
title_fullStr The antioxidant activity of a prenyl flavonoid alters its antifungal toxicity on Candida albicans biofilms
title_full_unstemmed The antioxidant activity of a prenyl flavonoid alters its antifungal toxicity on Candida albicans biofilms
title_sort The antioxidant activity of a prenyl flavonoid alters its antifungal toxicity on Candida albicans biofilms
dc.creator.none.fl_str_mv Peralta, Mariana Andrea
Ortega, María Gabriela
Cabrera, Jose Luis
Paraje, María Gabriela
author Peralta, Mariana Andrea
author_facet Peralta, Mariana Andrea
Ortega, María Gabriela
Cabrera, Jose Luis
Paraje, María Gabriela
author_role author
author2 Ortega, María Gabriela
Cabrera, Jose Luis
Paraje, María Gabriela
author2_role author
author
author
dc.subject.none.fl_str_mv CANDIDA ALBICANS BIOFILMS
CONFOCAL SCANNING LASER MICROSCOPY
DALEA ELEGANS
FREE RADICALS
PRENYL FLAVONOID
PROOXIDANT/ANTIOXIDANT ACTION
https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
topic CANDIDA ALBICANS BIOFILMS
CONFOCAL SCANNING LASER MICROSCOPY
DALEA ELEGANS
FREE RADICALS
PRENYL FLAVONOID
PROOXIDANT/ANTIOXIDANT ACTION
https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
description The antioxidant effect of 8PP, a prenylflavonoid from Dalea elegans on Candida albicans biofilms, was investigated. We previously reported that sensitive (SCa) and resistant C. albicans (RCa) biofilms were strongly inhibited by this compound, in a dose-depending manner (50 μM-100 μM), with a prooxidant effect leading to accumulation of endogenous oxidative metabolites and increased antioxidant defenses. In this work, the antifungal activity of high concentrations of 8PP (200–1000 μM), the cellular stress imbalance and the architecture of biofilms were evaluated. Biofilms were studied by crystal violet and confocal scanning laser microscopy (CSLM) with COMSTAT analysis. Superoxide anion radical, the activity of the superoxide dismutase and the total antioxidant capacity were measured. Intracellular ROS were detected by a DCFH-DA and visualized by CSLM; reactive nitrogen intermediates by Griess. An antioxidant effect was detected at 1000 μM and levels of oxidant metabolites remained low, with major changes in the SCa. COMSTAT analysis showed that biofilms treated with higher concentrations exhibited different diffusion distances with altered topographic surface architectures, voids, channels and pores that could change the flow inside the matrix of biofilms. We demonstrate for first time, a concentration-dependent antioxidant action of 8PP, which can alter its antifungal activity on biofilms.
publishDate 2018
dc.date.none.fl_str_mv 2018-04
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/86569
Peralta, Mariana Andrea; Ortega, María Gabriela; Cabrera, Jose Luis; Paraje, María Gabriela; The antioxidant activity of a prenyl flavonoid alters its antifungal toxicity on Candida albicans biofilms; Pergamon-Elsevier Science Ltd; Food And Chemical Toxicology; 114; 4-2018; 285-291
0278-6915
CONICET Digital
CONICET
url http://hdl.handle.net/11336/86569
identifier_str_mv Peralta, Mariana Andrea; Ortega, María Gabriela; Cabrera, Jose Luis; Paraje, María Gabriela; The antioxidant activity of a prenyl flavonoid alters its antifungal toxicity on Candida albicans biofilms; Pergamon-Elsevier Science Ltd; Food And Chemical Toxicology; 114; 4-2018; 285-291
0278-6915
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://linkinghub.elsevier.com/retrieve/pii/S0278691518301133
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.fct.2018.02.042
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Pergamon-Elsevier Science Ltd
publisher.none.fl_str_mv Pergamon-Elsevier Science Ltd
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1799196210704154624
spelling The antioxidant activity of a prenyl flavonoid alters its antifungal toxicity on Candida albicans biofilmsPeralta, Mariana AndreaOrtega, María GabrielaCabrera, Jose LuisParaje, María GabrielaCANDIDA ALBICANS BIOFILMSCONFOCAL SCANNING LASER MICROSCOPYDALEA ELEGANSFREE RADICALSPRENYL FLAVONOIDPROOXIDANT/ANTIOXIDANT ACTIONhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1The antioxidant effect of 8PP, a prenylflavonoid from Dalea elegans on Candida albicans biofilms, was investigated. We previously reported that sensitive (SCa) and resistant C. albicans (RCa) biofilms were strongly inhibited by this compound, in a dose-depending manner (50 μM-100 μM), with a prooxidant effect leading to accumulation of endogenous oxidative metabolites and increased antioxidant defenses. In this work, the antifungal activity of high concentrations of 8PP (200–1000 μM), the cellular stress imbalance and the architecture of biofilms were evaluated. Biofilms were studied by crystal violet and confocal scanning laser microscopy (CSLM) with COMSTAT analysis. Superoxide anion radical, the activity of the superoxide dismutase and the total antioxidant capacity were measured. Intracellular ROS were detected by a DCFH-DA and visualized by CSLM; reactive nitrogen intermediates by Griess. An antioxidant effect was detected at 1000 μM and levels of oxidant metabolites remained low, with major changes in the SCa. COMSTAT analysis showed that biofilms treated with higher concentrations exhibited different diffusion distances with altered topographic surface architectures, voids, channels and pores that could change the flow inside the matrix of biofilms. We demonstrate for first time, a concentration-dependent antioxidant action of 8PP, which can alter its antifungal activity on biofilms.Fil: Peralta, Mariana Andrea. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto Multidisciplinario de Biología Vegetal. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Instituto Multidisciplinario de Biología Vegetal; ArgentinaFil: Ortega, María Gabriela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto Multidisciplinario de Biología Vegetal. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Instituto Multidisciplinario de Biología Vegetal; ArgentinaFil: Cabrera, Jose Luis. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto Multidisciplinario de Biología Vegetal. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Instituto Multidisciplinario de Biología Vegetal; ArgentinaFil: Paraje, María Gabriela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto Multidisciplinario de Biología Vegetal. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Instituto Multidisciplinario de Biología Vegetal; ArgentinaPergamon-Elsevier Science Ltd2018-04info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/86569Peralta, Mariana Andrea; Ortega, María Gabriela; Cabrera, Jose Luis; Paraje, María Gabriela; The antioxidant activity of a prenyl flavonoid alters its antifungal toxicity on Candida albicans biofilms; Pergamon-Elsevier Science Ltd; Food And Chemical Toxicology; 114; 4-2018; 285-2910278-6915CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://linkinghub.elsevier.com/retrieve/pii/S0278691518301133info:eu-repo/semantics/altIdentifier/doi/10.1016/j.fct.2018.02.042info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2024-05-08T14:18:57Zoai:ri.conicet.gov.ar:11336/86569instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982024-05-08 14:18:57.936CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
score 15,812429

Similares en LA Referencia