Establishment of cell-based transposon-mediated transgenesis in cattle

Transposon-mediated transgenesis is a well-established tool for genome modification in small animal models. However, translation of this active transgenic method to large animals warrants further investigations. Here, the piggyBac (PB) and sleeping beauty (SB) transposon systems were assessed for st...

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Autores: Alessio, Ana Paula, Fili, Alejandro, Garrels, Wiebke, Forcato, Diego Oscar, Olmos Nicotra, Maria Florencia, Liaudat, Ana Cecilia, Bevacqua, Romina Jimena, Savy, Virginia, Hiriart, María Inés, Talluri, Thirumala R., Owens, Jesse B., Ivics, Zoltán, Salamone, Daniel Felipe, Moisyadi, Stefan, Kues, Wilfried A., Bosch, Pablo
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2016
País:Argentina
Institución:Consejo Nacional de Investigaciones Científicas y Técnicas
Repositorio:CONICET Digital (CONICET)
Idioma:inglés
OAI Identifier:oai:ri.conicet.gov.ar:11336/44624
Acceso en línea:http://hdl.handle.net/11336/44624
Access Level:acceso abierto
Palabra clave:PiggyBac
Sleeping beauty
Transposon
Cattle
Transgenesis
https://purl.org/becyt/ford/4.4
https://purl.org/becyt/ford/4
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oai_identifier_str oai:ri.conicet.gov.ar:11336/44624
network_acronym_str AR
network_name_str Argentina
repository_id_str
dc.title.none.fl_str_mv Establishment of cell-based transposon-mediated transgenesis in cattle
title Establishment of cell-based transposon-mediated transgenesis in cattle
spellingShingle Establishment of cell-based transposon-mediated transgenesis in cattle
Alessio, Ana Paula
PiggyBac
Sleeping beauty
Transposon
Cattle
Transgenesis
https://purl.org/becyt/ford/4.4
https://purl.org/becyt/ford/4
title_short Establishment of cell-based transposon-mediated transgenesis in cattle
title_full Establishment of cell-based transposon-mediated transgenesis in cattle
title_fullStr Establishment of cell-based transposon-mediated transgenesis in cattle
title_full_unstemmed Establishment of cell-based transposon-mediated transgenesis in cattle
title_sort Establishment of cell-based transposon-mediated transgenesis in cattle
dc.creator.none.fl_str_mv Alessio, Ana Paula
Fili, Alejandro
Garrels, Wiebke
Forcato, Diego Oscar
Olmos Nicotra, Maria Florencia
Liaudat, Ana Cecilia
Bevacqua, Romina Jimena
Savy, Virginia
Hiriart, María Inés
Talluri, Thirumala R.
Owens, Jesse B.
Ivics, Zoltán
Salamone, Daniel Felipe
Moisyadi, Stefan
Kues, Wilfried A.
Bosch, Pablo
author Alessio, Ana Paula
author_facet Alessio, Ana Paula
Fili, Alejandro
Garrels, Wiebke
Forcato, Diego Oscar
Olmos Nicotra, Maria Florencia
Liaudat, Ana Cecilia
Bevacqua, Romina Jimena
Savy, Virginia
Hiriart, María Inés
Talluri, Thirumala R.
Owens, Jesse B.
Ivics, Zoltán
Salamone, Daniel Felipe
Moisyadi, Stefan
Kues, Wilfried A.
Bosch, Pablo
author_role author
author2 Fili, Alejandro
Garrels, Wiebke
Forcato, Diego Oscar
Olmos Nicotra, Maria Florencia
Liaudat, Ana Cecilia
Bevacqua, Romina Jimena
Savy, Virginia
Hiriart, María Inés
Talluri, Thirumala R.
Owens, Jesse B.
Ivics, Zoltán
Salamone, Daniel Felipe
Moisyadi, Stefan
Kues, Wilfried A.
Bosch, Pablo
author2_role author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv PiggyBac
Sleeping beauty
Transposon
Cattle
Transgenesis
https://purl.org/becyt/ford/4.4
https://purl.org/becyt/ford/4
topic PiggyBac
Sleeping beauty
Transposon
Cattle
Transgenesis
https://purl.org/becyt/ford/4.4
https://purl.org/becyt/ford/4
description Transposon-mediated transgenesis is a well-established tool for genome modification in small animal models. However, translation of this active transgenic method to large animals warrants further investigations. Here, the piggyBac (PB) and sleeping beauty (SB) transposon systems were assessed for stable gene transfer into the cattle genome. Bovine fibroblasts were transfected either with a helper-independent PB system or a binary SB system. Both transposons were highly active in bovine cells increasing the efficiency of DNA integration up to 88 times over basal nonfacilitated integrations in a colony formation assay. SB transposase catalyzed multiplex transgene integrations in fibroblast cells transfected with the helper vector and two donor vectors carrying different transgenes (fluorophore and neomycin resistance). Stably transfected fibroblasts were used for SCNT and on in vitro embryo culture, morphologically normal blastocysts that expressed the fluorophore were obtained with both transposon systems. The data indicate that transpositionis a feasible approach for genetic engineering in the cattle genome.
publishDate 2016
dc.date.none.fl_str_mv 2016-04
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/44624
Alessio, Ana Paula; Fili, Alejandro; Garrels, Wiebke; Forcato, Diego Oscar; Olmos Nicotra, Maria Florencia; et al.; Establishment of cell-based transposon-mediated transgenesis in cattle; Elsevier Science Inc; Theriogenology; 85; 7; 4-2016; 1297-1311
0093-691X
1879-3231
CONICET Digital
CONICET
url http://hdl.handle.net/11336/44624
identifier_str_mv Alessio, Ana Paula; Fili, Alejandro; Garrels, Wiebke; Forcato, Diego Oscar; Olmos Nicotra, Maria Florencia; et al.; Establishment of cell-based transposon-mediated transgenesis in cattle; Elsevier Science Inc; Theriogenology; 85; 7; 4-2016; 1297-1311
0093-691X
1879-3231
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1016/j.theriogenology.2015.12.016
info:eu-repo/semantics/altIdentifier/url/https://www.theriojournal.com/article/S0093-691X(15)00691-3/fulltext
info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0093691X15006913
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier Science Inc
publisher.none.fl_str_mv Elsevier Science Inc
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1799195738520944640
spelling Establishment of cell-based transposon-mediated transgenesis in cattleAlessio, Ana PaulaFili, AlejandroGarrels, WiebkeForcato, Diego OscarOlmos Nicotra, Maria FlorenciaLiaudat, Ana CeciliaBevacqua, Romina JimenaSavy, VirginiaHiriart, María InésTalluri, Thirumala R.Owens, Jesse B.Ivics, ZoltánSalamone, Daniel FelipeMoisyadi, StefanKues, Wilfried A.Bosch, PabloPiggyBacSleeping beautyTransposonCattleTransgenesishttps://purl.org/becyt/ford/4.4https://purl.org/becyt/ford/4Transposon-mediated transgenesis is a well-established tool for genome modification in small animal models. However, translation of this active transgenic method to large animals warrants further investigations. Here, the piggyBac (PB) and sleeping beauty (SB) transposon systems were assessed for stable gene transfer into the cattle genome. Bovine fibroblasts were transfected either with a helper-independent PB system or a binary SB system. Both transposons were highly active in bovine cells increasing the efficiency of DNA integration up to 88 times over basal nonfacilitated integrations in a colony formation assay. SB transposase catalyzed multiplex transgene integrations in fibroblast cells transfected with the helper vector and two donor vectors carrying different transgenes (fluorophore and neomycin resistance). Stably transfected fibroblasts were used for SCNT and on in vitro embryo culture, morphologically normal blastocysts that expressed the fluorophore were obtained with both transposon systems. The data indicate that transpositionis a feasible approach for genetic engineering in the cattle genome.Fil: Alessio, Ana Paula. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario; Argentina. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Biología Molecular; ArgentinaFil: Fili, Alejandro. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario; Argentina. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Biología Molecular; ArgentinaFil: Garrels, Wiebke. Institut für Nutztiergenetik; Alemania. Gottfried Wilhelm Leibniz Universität Hannover; AlemaniaFil: Forcato, Diego Oscar. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario; Argentina. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Biología Molecular; ArgentinaFil: Olmos Nicotra, Maria Florencia. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario; Argentina. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Biología Molecular; ArgentinaFil: Liaudat, Ana Cecilia. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario; Argentina. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Biología Molecular; ArgentinaFil: Bevacqua, Romina Jimena. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario; Argentina. Universidad de Buenos Aires. Facultad de Agronomía. Pabellón de Zootecnica. Laboratorio de Biotecnología Animal; ArgentinaFil: Savy, Virginia. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario; Argentina. Universidad de Buenos Aires. Facultad de Agronomía. Pabellón de Zootecnica. Laboratorio de Biotecnología Animal; ArgentinaFil: Hiriart, María Inés. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario; Argentina. Universidad de Buenos Aires. Facultad de Agronomía. Pabellón de Zootecnica. Laboratorio de Biotecnología Animal; ArgentinaFil: Talluri, Thirumala R.. Institut für Nutztiergenetik; AlemaniaFil: Owens, Jesse B.. University of Hawaii at Manoa; Estados UnidosFil: Ivics, Zoltán. Paul-Ehrlich-Institute; AlemaniaFil: Salamone, Daniel Felipe. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario; Argentina. Universidad de Buenos Aires. Facultad de Agronomía. Pabellón de Zootecnica. Laboratorio de Biotecnología Animal; ArgentinaFil: Moisyadi, Stefan. University of Hawaii at Manoa; Estados UnidosFil: Kues, Wilfried A.. Institut für Nutztiergenetik; AlemaniaFil: Bosch, Pablo. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario; Argentina. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Biología Molecular; ArgentinaElsevier Science Inc2016-04info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/44624Alessio, Ana Paula; Fili, Alejandro; Garrels, Wiebke; Forcato, Diego Oscar; Olmos Nicotra, Maria Florencia; et al.; Establishment of cell-based transposon-mediated transgenesis in cattle; Elsevier Science Inc; Theriogenology; 85; 7; 4-2016; 1297-13110093-691X1879-3231CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1016/j.theriogenology.2015.12.016info:eu-repo/semantics/altIdentifier/url/https://www.theriojournal.com/article/S0093-691X(15)00691-3/fulltextinfo:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0093691X15006913info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-nd/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2024-05-08T14:02:00Zoai:ri.conicet.gov.ar:11336/44624instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982024-05-08 14:02:01.189CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
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