Comparative EvaluationIn vitroof the HerbicideFlurochloridone by Cytokinesis-blockMicronucleus Cytome and Comet Assays
Thein-vitroeffects of flurochloridone and its formulations Twin Pack Gold1(25% a.i.) andRainbow1(25% a.i.) were evaluated in Chinese Hamster Ovary K1 (CHO-K1) cells. The cytokinesis-blockmicronucleus cytome (CBMN-cyt) and single-cell gel electrophoresis (SCGE) assays were used. Theactivities were te...
| Autores: | , , |
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| Tipo de documento: | artigo |
| Estado: | Versão publicada |
| Data de publicação: | 2014 |
| País: | Argentina |
| Recursos: | Consejo Nacional de Investigaciones Científicas y Técnicas |
| Repositório: | CONICET Digital (CONICET) |
| Idioma: | inglês |
| OAI Identifier: | oai:ri.conicet.gov.ar:11336/32872 |
| Acesso em linha: | http://hdl.handle.net/11336/32872 |
| Access Level: | Acceso aberto |
| Palavra-chave: | Flurochloridone Cbmn-Cyt Assay Dna-Single Strands Breaks Cho-K1 Cells Commercial Formulations https://purl.org/becyt/ford/1.6 https://purl.org/becyt/ford/1 |
| Resumo: | Thein-vitroeffects of flurochloridone and its formulations Twin Pack Gold1(25% a.i.) andRainbow1(25% a.i.) were evaluated in Chinese Hamster Ovary K1 (CHO-K1) cells. The cytokinesis-blockmicronucleus cytome (CBMN-cyt) and single-cell gel electrophoresis (SCGE) assays were used. Theactivities were tested within the range of final concentrations of 0.25–15lg flurochloridone/mL. Theresults demonstrated that both the flurochloridone and Rainbow1were not able to induce micronuclei(MN). On the other hand, Twin Pack Gold1only increased the frequency of MN at 5lg/mL. Furthermore,10 and 15lg/mL of both formulations resulted in a cellular cytotoxicity demonstrated by alterations in thenuclear division index and cellular death. SCGE assay appeared to be a more sensitive bioassay fordetecting primary DNA strand breaks at lower concentrations of flurochloridone than MN did. A markedincrease in the genetic damage index was observed when 5 and 15lg/mL of both flurochloridone andRainbow1but only when 15lg/mL of Twin Pack Gold1were used. This is the first report demonstratingthat flurochloridone and its two commercial formulations are able to induce single-strand DNA breaksinvitroon mammalian cells. |
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