Lack of cytochrome c in Arabidopsis decreases stability of Complex IV and modifies redox metabolism without affecting Complexes i and III

We studied the role of cytochrome c (CYTc), which mediates electron transfer between Complexes III and IV, in cellular events related with mitochondrial respiration, plant development and redox homeostasis. We analyzed single and double homozygous mutants in both CYTc-encoding genes from Arabidopsis...

Descripción completa

Detalles Bibliográficos
Autores: Welchen, Elina, Hildebrandt, Tatjana M., Lewejohann, Dagmar, Gonzalez, Daniel Hector, Braun, Hans Peter
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2012
País:Argentina
Institución:Consejo Nacional de Investigaciones Científicas y Técnicas
Repositorio:CONICET Digital (CONICET)
Idioma:inglés
OAI Identifier:oai:ri.conicet.gov.ar:11336/60491
Acceso en línea:http://hdl.handle.net/11336/60491
Access Level:acceso abierto
Palabra clave:Arabidopsis Thaliana
Cytochrome C
Embryo Lethal
Mitochondrion
Respiratory Chain Complex
https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
id AR_400a1a0a5830c36cda0271b4e898614e
oai_identifier_str oai:ri.conicet.gov.ar:11336/60491
network_acronym_str AR
network_name_str Argentina
repository_id_str
spelling Lack of cytochrome c in Arabidopsis decreases stability of Complex IV and modifies redox metabolism without affecting Complexes i and IIIWelchen, ElinaHildebrandt, Tatjana M.Lewejohann, DagmarGonzalez, Daniel HectorBraun, Hans PeterArabidopsis ThalianaCytochrome CEmbryo LethalMitochondrionRespiratory Chain Complexhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1We studied the role of cytochrome c (CYTc), which mediates electron transfer between Complexes III and IV, in cellular events related with mitochondrial respiration, plant development and redox homeostasis. We analyzed single and double homozygous mutants in both CYTc-encoding genes from Arabidopsis: CYTC-1 and CYTC-2. While individual mutants were similar to wild-type, knock-out of both genes produced an arrest of embryo development, showing that CYTc function is essential at early stages of plant development. Mutants in which CYTc levels were extremely reduced respective to wild-type had smaller rosettes with a pronounced decrease in parenchymatic cell size and an overall delay in development. Mitochondria from these mutants had lower respiration rates and a relative increase in alternative respiration. Furthermore, the decrease in CYTc severely affected the activity and the amount of Complex IV, without affecting Complexes I and III. Reactive oxygen species levels were reduced in these mutants, which showed induction of genes encoding antioxidant enzymes. Ascorbic acid levels were not affected, suggesting that a small amount of CYTc is enough to support its normal synthesis. We postulate that, in addition to its role as an electron carrier between Complexes III and IV, CYTc influences Complex IV levels in plants, probably reflecting a role of this protein in Complex IV stability. This double function of CYTc most likely explains why it is essential for plant survival. © 2012 Elsevier B.V.Fil: Welchen, Elina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Agrobiotecnología del Litoral. Universidad Nacional del Litoral. Instituto de Agrobiotecnología del Litoral; ArgentinaFil: Hildebrandt, Tatjana M.. Leibniz Universität Hannover; AlemaniaFil: Lewejohann, Dagmar. Leibniz Universität Hannover; AlemaniaFil: Gonzalez, Daniel Hector. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Agrobiotecnología del Litoral. Universidad Nacional del Litoral. Instituto de Agrobiotecnología del Litoral; ArgentinaFil: Braun, Hans Peter. Leibniz Universität Hannover; AlemaniaElsevier Science2012-07info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/60491Welchen, Elina; Hildebrandt, Tatjana M.; Lewejohann, Dagmar; Gonzalez, Daniel Hector; Braun, Hans Peter; Lack of cytochrome c in Arabidopsis decreases stability of Complex IV and modifies redox metabolism without affecting Complexes i and III; Elsevier Science; Biochimica Et Biophysica Acta-bioenergetics; 1817; 7; 7-2012; 990-10010005-2728CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1016/j.bbabio.2012.04.008info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0005272812001302info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2024-05-08T14:27:24Zoai:ri.conicet.gov.ar:11336/60491instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982024-05-08 14:27:25.072CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Lack of cytochrome c in Arabidopsis decreases stability of Complex IV and modifies redox metabolism without affecting Complexes i and III
title Lack of cytochrome c in Arabidopsis decreases stability of Complex IV and modifies redox metabolism without affecting Complexes i and III
spellingShingle Lack of cytochrome c in Arabidopsis decreases stability of Complex IV and modifies redox metabolism without affecting Complexes i and III
Welchen, Elina
Arabidopsis Thaliana
Cytochrome C
Embryo Lethal
Mitochondrion
Respiratory Chain Complex
https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
title_short Lack of cytochrome c in Arabidopsis decreases stability of Complex IV and modifies redox metabolism without affecting Complexes i and III
title_full Lack of cytochrome c in Arabidopsis decreases stability of Complex IV and modifies redox metabolism without affecting Complexes i and III
title_fullStr Lack of cytochrome c in Arabidopsis decreases stability of Complex IV and modifies redox metabolism without affecting Complexes i and III
title_full_unstemmed Lack of cytochrome c in Arabidopsis decreases stability of Complex IV and modifies redox metabolism without affecting Complexes i and III
title_sort Lack of cytochrome c in Arabidopsis decreases stability of Complex IV and modifies redox metabolism without affecting Complexes i and III
dc.creator.none.fl_str_mv Welchen, Elina
Hildebrandt, Tatjana M.
Lewejohann, Dagmar
Gonzalez, Daniel Hector
Braun, Hans Peter
author Welchen, Elina
author_facet Welchen, Elina
Hildebrandt, Tatjana M.
Lewejohann, Dagmar
Gonzalez, Daniel Hector
Braun, Hans Peter
author_role author
author2 Hildebrandt, Tatjana M.
Lewejohann, Dagmar
Gonzalez, Daniel Hector
Braun, Hans Peter
author2_role author
author
author
author
dc.subject.none.fl_str_mv Arabidopsis Thaliana
Cytochrome C
Embryo Lethal
Mitochondrion
Respiratory Chain Complex
https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
topic Arabidopsis Thaliana
Cytochrome C
Embryo Lethal
Mitochondrion
Respiratory Chain Complex
https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
description We studied the role of cytochrome c (CYTc), which mediates electron transfer between Complexes III and IV, in cellular events related with mitochondrial respiration, plant development and redox homeostasis. We analyzed single and double homozygous mutants in both CYTc-encoding genes from Arabidopsis: CYTC-1 and CYTC-2. While individual mutants were similar to wild-type, knock-out of both genes produced an arrest of embryo development, showing that CYTc function is essential at early stages of plant development. Mutants in which CYTc levels were extremely reduced respective to wild-type had smaller rosettes with a pronounced decrease in parenchymatic cell size and an overall delay in development. Mitochondria from these mutants had lower respiration rates and a relative increase in alternative respiration. Furthermore, the decrease in CYTc severely affected the activity and the amount of Complex IV, without affecting Complexes I and III. Reactive oxygen species levels were reduced in these mutants, which showed induction of genes encoding antioxidant enzymes. Ascorbic acid levels were not affected, suggesting that a small amount of CYTc is enough to support its normal synthesis. We postulate that, in addition to its role as an electron carrier between Complexes III and IV, CYTc influences Complex IV levels in plants, probably reflecting a role of this protein in Complex IV stability. This double function of CYTc most likely explains why it is essential for plant survival. © 2012 Elsevier B.V.
publishDate 2012
dc.date.none.fl_str_mv 2012-07
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/60491
Welchen, Elina; Hildebrandt, Tatjana M.; Lewejohann, Dagmar; Gonzalez, Daniel Hector; Braun, Hans Peter; Lack of cytochrome c in Arabidopsis decreases stability of Complex IV and modifies redox metabolism without affecting Complexes i and III; Elsevier Science; Biochimica Et Biophysica Acta-bioenergetics; 1817; 7; 7-2012; 990-1001
0005-2728
CONICET Digital
CONICET
url http://hdl.handle.net/11336/60491
identifier_str_mv Welchen, Elina; Hildebrandt, Tatjana M.; Lewejohann, Dagmar; Gonzalez, Daniel Hector; Braun, Hans Peter; Lack of cytochrome c in Arabidopsis decreases stability of Complex IV and modifies redox metabolism without affecting Complexes i and III; Elsevier Science; Biochimica Et Biophysica Acta-bioenergetics; 1817; 7; 7-2012; 990-1001
0005-2728
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1016/j.bbabio.2012.04.008
info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0005272812001302
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier Science
publisher.none.fl_str_mv Elsevier Science
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1799196429146652672
score 15,811543

Similares en LA Referencia