Decay-Accelerating Factor 1 Deficiency Exacerbates Leptospiral-Induced Murine Chronic Nephritis and Renal Fibrosis
Leptospirosis is a global zoonosis caused by pathogenicLeptospira, which can colonize the proximal renal tubules andpersist for long periods in the kidneys of infected hosts. Here, we characterized the infection of C57BL/6J wild-type andDaf12/2mice, which have an enhanced host response, with a virul...
| Autores: | , , , , , , , |
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| Tipo de recurso: | artículo |
| Estado: | Versión publicada |
| Fecha de publicación: | 2014 |
| País: | Argentina |
| Institución: | Consejo Nacional de Investigaciones Científicas y Técnicas |
| Repositorio: | CONICET Digital (CONICET) |
| Idioma: | inglés |
| OAI Identifier: | oai:ri.conicet.gov.ar:11336/33244 |
| Acceso en línea: | http://hdl.handle.net/11336/33244 |
| Access Level: | acceso abierto |
| Palabra clave: | Leptospira Interrogans Daf1 Knock-Out Chronic Murine Model Fibrosis https://purl.org/becyt/ford/3.1 https://purl.org/becyt/ford/3 |
| Sumario: | Leptospirosis is a global zoonosis caused by pathogenicLeptospira, which can colonize the proximal renal tubules andpersist for long periods in the kidneys of infected hosts. Here, we characterized the infection of C57BL/6J wild-type andDaf12/2mice, which have an enhanced host response, with a virulentLeptospira interrogansstrain at 14 days post-infection,its persistence in the kidney, and its link to kidney fibrosis at 90 days post-infection. We found thatLeptospira interroganscan induce acute moderate nephritis in wild-type mice and is able to persist in some animals, inducing fibrosis in theabsence of mortality. In contrast, Daf12/2mice showed acute mortality, with a higher bacterial burden. At the chronic stage,Daf12/2mice showed greater inflammation and fibrosis than at 14 days post-infection and higher levels at all times thanthe wild-type counterpart. Compared with uninfected mice, infected wild-type mice showed higher levels of IL-4, IL-10 andIL-13, with similar levels ofa-smooth muscle actin, galectin-3, TGF-b1, IL-17, IFN-c, and lower IL-12 levels at 90 days post-infection. In contrast, fibrosis in Daf12/2mice was accompanied by high expression ofa-smooth muscle actin, galectin-3, IL-10, IL-13, and IFN-c, similar levels of TGF-b1, IL-12, and IL-17 and lower IL-4 levels. This study demonstrates the link betweenLeptospira-induced murine chronic nephritis with renal fibrosis and shows a protective role of Daf1. |
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