Characterization of β-Glucosidase produced by the white rot fungus Flammulina velutipes
β-glucosidase production by the white rot fungus Flammulina velutipes CFK 3111 was evaluated using different carbon and nitrogen sources under submerged fermentation. Maximal extracellular enzyme production was 1.6 U/mL corresponding to a culture grown in sucrose 40 g/L and asparagine 10 g/L. High p...
| Autores: | , , |
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| Tipo de recurso: | artículo |
| Estado: | Versión publicada |
| Fecha de publicación: | 2014 |
| País: | Argentina |
| Institución: | Consejo Nacional de Investigaciones Científicas y Técnicas |
| Repositorio: | CONICET Digital (CONICET) |
| Idioma: | inglés |
| OAI Identifier: | oai:ri.conicet.gov.ar:11336/16733 |
| Acceso en línea: | http://hdl.handle.net/11336/16733 |
| Access Level: | acceso abierto |
| Palabra clave: | B-Glucosidase Flammulina Velutipes Submerged Fermentation https://purl.org/becyt/ford/1.6 https://purl.org/becyt/ford/1 |
| Sumario: | β-glucosidase production by the white rot fungus Flammulina velutipes CFK 3111 was evaluated using different carbon and nitrogen sources under submerged fermentation. Maximal extracellular enzyme production was 1.6 U/mL corresponding to a culture grown in sucrose 40 g/L and asparagine 10 g/L. High production yield was also obtained with glucose 10 g/L and asparagine 4 g/L medium (0.5 U/mL). Parameters affecting enzyme activity were studied using p-nitro-phenyl-β-D-glucopyranoside as substrate. Optimal activity was found at 50°C and pHs 5.0 to 6.0. Under these conditions, β-glucosidase retained 25% of its initial activity after 12 h of incubation and exhibited a half-life of 5 h. The addition of MgCl2, urea and ethanol enhanced β-glucosidase activity up to 47% while the reactives FeCl2, CuSO4, Cd(NO3)2 and cetyltrimethylammonium bromide inflicted a strong inhibitory effect. Glucose and cellobiose showed also an inhibitory effect on β-glucosidase activity in a concentration dependent manner. The enzyme had an estimated molecular mass of 75 kDa. To the best of our knowledge, F. velutipes CFK 3111 β -glucosidase production is amongst the highest reported up to date, in a basidiomycetous fungus. |
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