Chemotactic response of the flavonoid daidzein and its effect on the composition of the rumen bacterial community

Daidzein is one of common metabolites in plants and has chemotactic effect on soil bacteria that colonize the plants. There are several tests to assess bacterial chemotaxis, but none focused on rumen bacteria. Therefore, the aim of this study was to test the chemotactic response of the rumen microfl...

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Detalles Bibliográficos
Autores: Galicia Jiménez, Mónica M., Rojas Herrera, Rafael, Sandoval Castro, Carlos, Murialdo, Silvia Elena, Magaña Sevilla, Héctor
Tipo de recurso: artículo
Estado:Versión enviada para evaluación y publicación
Fecha de publicación:2014
País:Argentina
Institución:Comisión de Investigaciones Científicas de la Provincia de Buenos Aires
Repositorio:CIC Digital (CICBA)
Idioma:inglés
OAI Identifier:oai:digital.cic.gba.gob.ar:11746/4077
Acceso en línea:https://digital.cic.gba.gob.ar/handle/11746/4077
Access Level:acceso abierto
Palabra clave:Bioquímica y Biología Molecular
Daidzein
ruminal bacteria
Celulosa
Quimiotaxis
Descripción
Sumario:Daidzein is one of common metabolites in plants and has chemotactic effect on soil bacteria that colonize the plants. There are several tests to assess bacterial chemotaxis, but none focused on rumen bacteria. Therefore, the aim of this study was to test the chemotactic response of the rumen microflora towards daidzein using a standardized bacterial chemotaxis assay. It consisted in a modifying capillary technique and employing technology for measuring in vitro gas production. Ruminal fluids and cellulose were used as controls. The response of bacteria to daidzein was greater than the response to cellulose, supporting the hypothesis that when fodder is chewed by the ruminant it releases daidzein which can attract rumen bacteria towards feed particles (chemotaxis) for attachment and subsequent degradation.