A phylogenetically conserved group of NF-Y transcription factors interact to control nodulation in legumes
The endosymbiotic association between legumes and rhizobia leads to the formation of root nodules in which differentiated bacteria convert atmospheric nitrogen into a form that can be assimilated by the host plant. Successful root infection by rhizobia and nodule organogenesis require the activation...
| Autores: | , , , , , , , , , , , |
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| Tipo de recurso: | artículo |
| Estado: | Versión publicada |
| Fecha de publicación: | 2015 |
| País: | Argentina |
| Institución: | Consejo Nacional de Investigaciones Científicas y Técnicas |
| Repositorio: | CONICET Digital (CONICET) |
| Idioma: | inglés |
| OAI Identifier: | oai:ri.conicet.gov.ar:11336/48109 |
| Acceso en línea: | http://hdl.handle.net/11336/48109 |
| Access Level: | acceso abierto |
| Palabra clave: | Transcription factors nodulation medicago symbiosis https://purl.org/becyt/ford/1.6 https://purl.org/becyt/ford/1 |
| Sumario: | The endosymbiotic association between legumes and rhizobia leads to the formation of root nodules in which differentiated bacteria convert atmospheric nitrogen into a form that can be assimilated by the host plant. Successful root infection by rhizobia and nodule organogenesis require the activation of symbiotic genes that are controlled by a set of early transcription factors (TFs). MtNF-YA1 and MtNF-YA2 are two TFs playing partially redundant functions during several steps of the symbiotic interaction between Medicago truncatula and Sinorhizobium meliloti. NF-Y proteins are part of a transcriptional complex composed of three proteins (NF-YA, NF-YB and NF-YC) which bind DNA at CCAAT-boxes, a motif present in most eukaryotic promoters. In plants, each subunit is encoded by small gene families, potentially leading to a multitude of heterotrimeric NF-Y complexes. Here, using yeast two hybrid screenings, we identified the MtNF-YB and MtNF-YC subunits that interact with MtNF-YA1 and A2. Further, we confirmed, both in yeast and in planta, the formation of trimeric NF-Y complexes and showed that these complexes are functional during nodulation using reverse genetic approaches and ChIP-PCR. Finally, as orthologs of the characterized NF-Y subunits also control nodulation in other legumes, we showed in common bean that similar NF-Y trimers could form in planta. Our results suggest that we have identified a group of evolutionary conserved NF-Y proteins that interact to control nodulation in leguminous plants. |
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