Ophioviruses CPsV and MiLBVV movement protein is encoded in RNA 2 and interacts with the coat protein
<i>Citrus psorosis virus</i> (CPsV) and <i>Mirafiori lettuce big-vein virus</i> (MiLBVV), members of the <i>Ophioviridae</i> family, have segmented negative-sense single-stranded RNA genomes. To date no reports have described how ophioviruses spread within host pl...
| Autores: | , , , , |
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| Tipo de recurso: | artículo |
| Estado: | Versión publicada |
| Fecha de publicación: | 2013 |
| País: | Argentina |
| Institución: | Universidad Nacional de La Plata |
| Repositorio: | SEDICI (UNLP) |
| Idioma: | inglés |
| OAI Identifier: | oai:sedici.unlp.edu.ar:10915/85543 |
| Acceso en línea: | http://sedici.unlp.edu.ar/handle/10915/85543 |
| Access Level: | acceso abierto |
| Palabra clave: | Ciencias Exactas Citrus psorosis virus Mirafiori lettuce big-vein virus Movement protein Negative-strand RNA virus Ophioviridae Protein-protein Interaction |
| Sumario: | <i>Citrus psorosis virus</i> (CPsV) and <i>Mirafiori lettuce big-vein virus</i> (MiLBVV), members of the <i>Ophioviridae</i> family, have segmented negative-sense single-stranded RNA genomes. To date no reports have described how ophioviruses spread within host plants and/or the proteins involved in this process. Here we show that the 54K protein of CPsV is encoded by RNA 2 and describe its subcellular distribution. Upon transient expression in <i>Nicotiana benthamiana</i> epidermal cells the 54K protein, and also its 54K counterpart protein of MiLBVV, localize to plasmodesmata and enhance GFP cell-to-cell diffusion between cells. Both proteins, but not the coat proteins (CP) of the respective viruses, functionally <i>trans</i>-complement cell-to-cell movement-defective <i>Potato virus</i> X (PVX) and <i>Tobacco mosaic virus</i> (TMV) mutants. The 54K and 54K proteins interact with the virus-specific CP in the cytoplasm, suggesting a potential role of CP in ophiovirus movement. This is the first study characterizing the movement proteins (MP) of ophioviruses. |
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