Implications of the expression of Enterococcus faecalis citrate fermentation genes during infection

Citrate is an ubiquitous compound in nature. However, citrate fermentation is present only in a few pathogenic or nonpathogenic microorganisms. The citrate fermentation pathway includes a citrate transporter, a citrate lyase complex, an oxaloacetate decarboxylase and a regulatory system. Enterococcu...

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Detalles Bibliográficos
Autores: Martino, Gabriela Paula, Perez Dezen, Cristian Emanuel, Magni, Christian, Blancato, Victor Sebastian
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2018
País:Argentina
Institución:Consejo Nacional de Investigaciones Científicas y Técnicas
Repositorio:CONICET Digital (CONICET)
Idioma:inglés
OAI Identifier:oai:ri.conicet.gov.ar:11336/88504
Acceso en línea:http://hdl.handle.net/11336/88504
Access Level:acceso abierto
Palabra clave:citrate fermentation
gene expression
infection
Enterococcus faecalis; Galleria Mellonella
https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
id AR_36a6943fdd5e8e48218cb4c19251fd5b
oai_identifier_str oai:ri.conicet.gov.ar:11336/88504
network_acronym_str AR
network_name_str Argentina
repository_id_str
dc.title.none.fl_str_mv Implications of the expression of Enterococcus faecalis citrate fermentation genes during infection
title Implications of the expression of Enterococcus faecalis citrate fermentation genes during infection
spellingShingle Implications of the expression of Enterococcus faecalis citrate fermentation genes during infection
Martino, Gabriela Paula
citrate fermentation
gene expression
infection
Enterococcus faecalis; Galleria Mellonella
https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
title_short Implications of the expression of Enterococcus faecalis citrate fermentation genes during infection
title_full Implications of the expression of Enterococcus faecalis citrate fermentation genes during infection
title_fullStr Implications of the expression of Enterococcus faecalis citrate fermentation genes during infection
title_full_unstemmed Implications of the expression of Enterococcus faecalis citrate fermentation genes during infection
title_sort Implications of the expression of Enterococcus faecalis citrate fermentation genes during infection
dc.creator.none.fl_str_mv Martino, Gabriela Paula
Perez Dezen, Cristian Emanuel
Magni, Christian
Blancato, Victor Sebastian
author Martino, Gabriela Paula
author_facet Martino, Gabriela Paula
Perez Dezen, Cristian Emanuel
Magni, Christian
Blancato, Victor Sebastian
author_role author
author2 Perez Dezen, Cristian Emanuel
Magni, Christian
Blancato, Victor Sebastian
author2_role author
author
author
dc.subject.none.fl_str_mv citrate fermentation
gene expression
infection
Enterococcus faecalis; Galleria Mellonella
https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
topic citrate fermentation
gene expression
infection
Enterococcus faecalis; Galleria Mellonella
https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
description Citrate is an ubiquitous compound in nature. However, citrate fermentation is present only in a few pathogenic or nonpathogenic microorganisms. The citrate fermentation pathway includes a citrate transporter, a citrate lyase complex, an oxaloacetate decarboxylase and a regulatory system. Enterococcus faecalis is commonly present in the gastro-intestinal microbiota of warm-blooded animals and insect guts. These bacteria can also cause infection and disease in immunocompromised individuals. In the present study, we performed whole genome analysis in Enterococcus strains finding that the complete citrate pathway is present in all of the E. faecalis strains isolated from such diverse habitats as animals, hospitals, water, milk, plants, insects, cheese, etc. These results indicate the importance of this metabolic preservation for persistence and growth of E. faecalis in different niches. We also analyzed the role of citrate metabolism in the E. faecalis pathogenicity. We found that an E. faecalis citrate fermentation-deficient strain was less pathogenic for Galleria mellonella larvae than the wild type. Furthermore, strains with deletions in the oxaloacetate decarboxylase subunits or in the ∞-acetolactate synthase resulted also less virulent than the wild type strain. We also observed that citrate promoters are induced in blood, urine and also in the hemolymph of G. mellonella. In addition, we showed that citrate fermentation allows E. faecalis to grow better in blood, urine and G. mellonella. The results presented here clearly indicate that citrate fermentation plays an important role in E. faecalis opportunistic pathogenic behavior.
publishDate 2018
dc.date.none.fl_str_mv 2018-10
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/88504
Martino, Gabriela Paula; Perez Dezen, Cristian Emanuel; Magni, Christian; Blancato, Victor Sebastian; Implications of the expression of Enterococcus faecalis citrate fermentation genes during infection; Public Library of Science; Plos One; 13; 10; 10-2018; 1-18; e0205787
1932-6203
CONICET Digital
CONICET
url http://hdl.handle.net/11336/88504
identifier_str_mv Martino, Gabriela Paula; Perez Dezen, Cristian Emanuel; Magni, Christian; Blancato, Victor Sebastian; Implications of the expression of Enterococcus faecalis citrate fermentation genes during infection; Public Library of Science; Plos One; 13; 10; 10-2018; 1-18; e0205787
1932-6203
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1371/journal.pone.0205787
info:eu-repo/semantics/altIdentifier/url/https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0205787
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Public Library of Science
publisher.none.fl_str_mv Public Library of Science
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1799195092767997952
spelling Implications of the expression of Enterococcus faecalis citrate fermentation genes during infectionMartino, Gabriela PaulaPerez Dezen, Cristian EmanuelMagni, ChristianBlancato, Victor Sebastiancitrate fermentationgene expressioninfectionEnterococcus faecalis; Galleria Mellonellahttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Citrate is an ubiquitous compound in nature. However, citrate fermentation is present only in a few pathogenic or nonpathogenic microorganisms. The citrate fermentation pathway includes a citrate transporter, a citrate lyase complex, an oxaloacetate decarboxylase and a regulatory system. Enterococcus faecalis is commonly present in the gastro-intestinal microbiota of warm-blooded animals and insect guts. These bacteria can also cause infection and disease in immunocompromised individuals. In the present study, we performed whole genome analysis in Enterococcus strains finding that the complete citrate pathway is present in all of the E. faecalis strains isolated from such diverse habitats as animals, hospitals, water, milk, plants, insects, cheese, etc. These results indicate the importance of this metabolic preservation for persistence and growth of E. faecalis in different niches. We also analyzed the role of citrate metabolism in the E. faecalis pathogenicity. We found that an E. faecalis citrate fermentation-deficient strain was less pathogenic for Galleria mellonella larvae than the wild type. Furthermore, strains with deletions in the oxaloacetate decarboxylase subunits or in the ∞-acetolactate synthase resulted also less virulent than the wild type strain. We also observed that citrate promoters are induced in blood, urine and also in the hemolymph of G. mellonella. In addition, we showed that citrate fermentation allows E. faecalis to grow better in blood, urine and G. mellonella. The results presented here clearly indicate that citrate fermentation plays an important role in E. faecalis opportunistic pathogenic behavior.Fil: Martino, Gabriela Paula. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Biología Molecular y Celular de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario; ArgentinaFil: Perez Dezen, Cristian Emanuel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Biología Molecular y Celular de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario; ArgentinaFil: Magni, Christian. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Biología Molecular y Celular de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario; ArgentinaFil: Blancato, Victor Sebastian. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Biología Molecular y Celular de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario; ArgentinaPublic Library of Science2018-10info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/88504Martino, Gabriela Paula; Perez Dezen, Cristian Emanuel; Magni, Christian; Blancato, Victor Sebastian; Implications of the expression of Enterococcus faecalis citrate fermentation genes during infection; Public Library of Science; Plos One; 13; 10; 10-2018; 1-18; e02057871932-6203CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1371/journal.pone.0205787info:eu-repo/semantics/altIdentifier/url/https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0205787info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2024-05-08T13:43:18Zoai:ri.conicet.gov.ar:11336/88504instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982024-05-08 13:43:19.101CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
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