Phosphatidylinositol 4-phosphate is associated to extracellular lipoproteic fractions and is detected in tomato apoplastic fluids

We have recently detected phosphatidylinositol-4-phosphate (PI4P) in the extracellular medium of tomato cell suspensions. Extracellular PI4P was shown to trigger the activation of defence responses induced by the fungal elicitor xylanase. In this study, by applying a differential centrifugation tech...

Descripción completa

Detalles Bibliográficos
Autores: Gonorazky, Ana Gabriela, Laxalt, Ana Maria, Dekker, H. L., Rep, M., Munnik, T., Testerink, C., de la Canal, L
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2012
País:Argentina
Institución:Consejo Nacional de Investigaciones Científicas y Técnicas
Repositorio:CONICET Digital (CONICET)
Idioma:inglés
OAI Identifier:oai:ri.conicet.gov.ar:11336/13122
Acceso en línea:http://hdl.handle.net/11336/13122
Access Level:acceso abierto
Palabra clave:Apoplast
Extracellular Medium
Lipid Signalling
Lysophospholipid
Phosphatidic Acid
Phosphatidylinositol 4-Phosphate
Phosphoinositide
https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
id AR_367ca989feba5dc2b2430a2c220aa6bc
oai_identifier_str oai:ri.conicet.gov.ar:11336/13122
network_acronym_str AR
network_name_str Argentina
repository_id_str
spelling Phosphatidylinositol 4-phosphate is associated to extracellular lipoproteic fractions and is detected in tomato apoplastic fluidsGonorazky, Ana GabrielaLaxalt, Ana MariaDekker, H. L.Rep, M.Munnik, T.Testerink, C.de la Canal, LApoplastExtracellular MediumLipid SignallingLysophospholipidPhosphatidic AcidPhosphatidylinositol 4-PhosphatePhosphoinositidehttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1We have recently detected phosphatidylinositol-4-phosphate (PI4P) in the extracellular medium of tomato cell suspensions. Extracellular PI4P was shown to trigger the activation of defence responses induced by the fungal elicitor xylanase. In this study, by applying a differential centrifugation technique, we found that extracellular PI4P is associated with fractions composed of diverse phospholipids and proteins, which were pelleted from the extracellular medium of tomato cell suspensions grown under basal conditions. Using mass spectrometry, we identified the proteins present in these pelleted fractions. Most of these proteins have previously been characterised as having a role in defence responses. Next, we evaluated whether PI4P could also be detected in an entire plant system. For this, apoplastic fluids of tomato plants grown under basal conditions were analysed using a lipid overlay assay. Interestingly, PI4P could be detected in intercellular fluids obtained from tomato leaflets and xylem sap of tomato plants. By employing electrospray ionisation tandem mass spectrometry (ESI-MS/MS), other phospholipids were also found in intercellular fluids of tomato plants. These had a markedly different profile from the phospholipid pattern identified in entire leaflets. Based on these results, the potential role of extracellular phospholipids in plant intercellular communication is discussed.Fil: Gonorazky, Ana Gabriela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Mar del Plata. Instituto de Investigaciones Biológicas; Argentina. Universidad Nacional de Mar del Plata; ArgentinaFil: Laxalt, Ana Maria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Mar del Plata. Instituto de Investigaciones Biológicas; Argentina. Universidad Nacional de Mar del Plata; ArgentinaFil: Dekker, H. L.. University Of Amsterdam; Países BajosFil: Rep, M.. University Of Amsterdam; Países BajosFil: Munnik, T.. University Of Amsterdam; Países BajosFil: Testerink, C.. University Of Amsterdam; Países BajosFil: de la Canal, L. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Mar del Plata. Instituto de Investigaciones Biológicas; Argentina. Universidad Nacional de Mar del Plata; ArgentinaWiley2012-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/13122Gonorazky, Ana Gabriela; Laxalt, Ana Maria; Dekker, H. L.; Rep, M.; Munnik, T.; et al.; Phosphatidylinositol 4-phosphate is associated to extracellular lipoproteic fractions and is detected in tomato apoplastic fluids; Wiley; Plant Biology; 14; 1; 1-2012; 41-491435-86031438-8677enginfo:eu-repo/semantics/altIdentifier/url/http://onlinelibrary.wiley.com/doi/10.1111/j.1438-8677.2011.00488.x/abstractinfo:eu-repo/semantics/altIdentifier/doi/10.1111/j.1438-8677.2011.00488.xinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2024-05-08T13:54:02Zoai:ri.conicet.gov.ar:11336/13122instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982024-05-08 13:54:03.237CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Phosphatidylinositol 4-phosphate is associated to extracellular lipoproteic fractions and is detected in tomato apoplastic fluids
title Phosphatidylinositol 4-phosphate is associated to extracellular lipoproteic fractions and is detected in tomato apoplastic fluids
spellingShingle Phosphatidylinositol 4-phosphate is associated to extracellular lipoproteic fractions and is detected in tomato apoplastic fluids
Gonorazky, Ana Gabriela
Apoplast
Extracellular Medium
Lipid Signalling
Lysophospholipid
Phosphatidic Acid
Phosphatidylinositol 4-Phosphate
Phosphoinositide
https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
title_short Phosphatidylinositol 4-phosphate is associated to extracellular lipoproteic fractions and is detected in tomato apoplastic fluids
title_full Phosphatidylinositol 4-phosphate is associated to extracellular lipoproteic fractions and is detected in tomato apoplastic fluids
title_fullStr Phosphatidylinositol 4-phosphate is associated to extracellular lipoproteic fractions and is detected in tomato apoplastic fluids
title_full_unstemmed Phosphatidylinositol 4-phosphate is associated to extracellular lipoproteic fractions and is detected in tomato apoplastic fluids
title_sort Phosphatidylinositol 4-phosphate is associated to extracellular lipoproteic fractions and is detected in tomato apoplastic fluids
dc.creator.none.fl_str_mv Gonorazky, Ana Gabriela
Laxalt, Ana Maria
Dekker, H. L.
Rep, M.
Munnik, T.
Testerink, C.
de la Canal, L
author Gonorazky, Ana Gabriela
author_facet Gonorazky, Ana Gabriela
Laxalt, Ana Maria
Dekker, H. L.
Rep, M.
Munnik, T.
Testerink, C.
de la Canal, L
author_role author
author2 Laxalt, Ana Maria
Dekker, H. L.
Rep, M.
Munnik, T.
Testerink, C.
de la Canal, L
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv Apoplast
Extracellular Medium
Lipid Signalling
Lysophospholipid
Phosphatidic Acid
Phosphatidylinositol 4-Phosphate
Phosphoinositide
https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
topic Apoplast
Extracellular Medium
Lipid Signalling
Lysophospholipid
Phosphatidic Acid
Phosphatidylinositol 4-Phosphate
Phosphoinositide
https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
description We have recently detected phosphatidylinositol-4-phosphate (PI4P) in the extracellular medium of tomato cell suspensions. Extracellular PI4P was shown to trigger the activation of defence responses induced by the fungal elicitor xylanase. In this study, by applying a differential centrifugation technique, we found that extracellular PI4P is associated with fractions composed of diverse phospholipids and proteins, which were pelleted from the extracellular medium of tomato cell suspensions grown under basal conditions. Using mass spectrometry, we identified the proteins present in these pelleted fractions. Most of these proteins have previously been characterised as having a role in defence responses. Next, we evaluated whether PI4P could also be detected in an entire plant system. For this, apoplastic fluids of tomato plants grown under basal conditions were analysed using a lipid overlay assay. Interestingly, PI4P could be detected in intercellular fluids obtained from tomato leaflets and xylem sap of tomato plants. By employing electrospray ionisation tandem mass spectrometry (ESI-MS/MS), other phospholipids were also found in intercellular fluids of tomato plants. These had a markedly different profile from the phospholipid pattern identified in entire leaflets. Based on these results, the potential role of extracellular phospholipids in plant intercellular communication is discussed.
publishDate 2012
dc.date.none.fl_str_mv 2012-01
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/13122
Gonorazky, Ana Gabriela; Laxalt, Ana Maria; Dekker, H. L.; Rep, M.; Munnik, T.; et al.; Phosphatidylinositol 4-phosphate is associated to extracellular lipoproteic fractions and is detected in tomato apoplastic fluids; Wiley; Plant Biology; 14; 1; 1-2012; 41-49
1435-8603
1438-8677
url http://hdl.handle.net/11336/13122
identifier_str_mv Gonorazky, Ana Gabriela; Laxalt, Ana Maria; Dekker, H. L.; Rep, M.; Munnik, T.; et al.; Phosphatidylinositol 4-phosphate is associated to extracellular lipoproteic fractions and is detected in tomato apoplastic fluids; Wiley; Plant Biology; 14; 1; 1-2012; 41-49
1435-8603
1438-8677
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://onlinelibrary.wiley.com/doi/10.1111/j.1438-8677.2011.00488.x/abstract
info:eu-repo/semantics/altIdentifier/doi/10.1111/j.1438-8677.2011.00488.x
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Wiley
publisher.none.fl_str_mv Wiley
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1799195488846610432
score 15.811543

Similares en LA Referencia