Desarrollo y estandarización de un ELISA indirecto para la detección de anticuerpos contra el Herpesvirus bovino tipo 1
An indirect enzyme linked immunosorbent assay (I-ELISA) was developed to detect antibodies to BHV-1 in serum samples. Nonidet P40-solubilized infected with BHV-I reference strain and mock-infected cell lysates were used as antigens. Serum samples were diluted 1: 64 and a commercial horse radish pero...
| Authors: | , , , , , |
|---|---|
| Format: | article |
| Status: | Published version |
| Publication Date: | 1999 |
| Country: | Argentina |
| Institution: | Comisión de Investigaciones Científicas de la Provincia de Buenos Aires |
| Repository: | CIC Digital (CICBA) |
| Language: | Spanish |
| OAI Identifier: | oai:digital.cic.gba.gob.ar:11746/8597 |
| Online Access: | https://digital.cic.gba.gob.ar/handle/11746/8597 |
| Access Level: | Open access |
| Keyword: | Ciencias Veterinarias Herpesvirus bovino 1; diagnóstico; ELISA indirecto Bovine Herpesvirus 1; diagnosis; indirect ELISA |
| Summary: | An indirect enzyme linked immunosorbent assay (I-ELISA) was developed to detect antibodies to BHV-1 in serum samples. Nonidet P40-solubilized infected with BHV-I reference strain and mock-infected cell lysates were used as antigens. Serum samples were diluted 1: 64 and a commercial horse radish peroxidase-labelled rabbit anti-bovine lgG was used as second antibody. The reaction was developed using azino-dietilbenzotiazol-sulfonate (ABTS). Cut-off was determined by ratio sample (Rs). Virus neutralization (VN) test was used as a reference test over the 587 samples, 416 of them were positive by VN and 1-EL1SA, 145 were negative by both techniques, and 26 were positive by 1-EL1SA and negative by VN. Using VN as standard, the ELISA showed a relative specificity and sensibility of 100 and 84.79%, respectively. |
|---|