In vitro conversion of saturated to monounsaturated fatty acid by Ehrlich ascites cells

In this paper, evidence is presented on the capacity of Ehrlich ascites cells to synthesize in vitro monounsaturated fatty acids from radioactive palmitate. Localization of the double bond was determined by ozonolysis and subsequent reduction of the ozonides to aldesters followed by gas liquid chrom...

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Detalles Bibliográficos
Autores: Mercuri, Osvaldo Francisco, De Tomás, María Elena, Antueno, Roberto Justo de
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:1981
País:Argentina
Institución:Universidad Nacional de La Plata
Repositorio:SEDICI (UNLP)
Idioma:inglés
OAI Identifier:oai:sedici.unlp.edu.ar:10915/146070
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/146070
Access Level:acceso abierto
Palabra clave:Bioquímica
Ehrlich ascites cells
Fatty acid
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spelling In vitro conversion of saturated to monounsaturated fatty acid by Ehrlich ascites cellsMercuri, Osvaldo FranciscoDe Tomás, María ElenaAntueno, Roberto Justo deBioquímicaEhrlich ascites cellsFatty acidIn this paper, evidence is presented on the capacity of Ehrlich ascites cells to synthesize in vitro monounsaturated fatty acids from radioactive palmitate. Localization of the double bond was determined by ozonolysis and subsequent reduction of the ozonides to aldesters followed by gas liquid chromatography. These results proved that Ehrlich ascites cells have a Δ9 desaturase that catalyzes the biosynthesis of palmitoleic acid from palmitic acid and oleic and vaccenic acid via elongation-desaturation and desaturation-elongation, respectively, using palmitic acid as substrate. Furthermore, it is shown that, as in the hepatic cells, Δ9 desaturase enzyme activity of the tumoral cells is associated with the endoplasmic reticulum. The electron transport components involved in the desaturase system, i.e., NADH-cytochrome bs reductase and NADH-cytochrome bs reductase, were also measured. The activities of these enzymes do not appear to be rate-limiting in the desaturase activity of these tumoral cells.Facultad de Ciencias Médicas1981info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionArticulohttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdf893-896http://sedici.unlp.edu.ar/handle/10915/146070enginfo:eu-repo/semantics/altIdentifier/issn/0024-4201info:eu-repo/semantics/altIdentifier/issn/1558-9307info:eu-repo/semantics/altIdentifier/doi/10.1007/bf02534994info:eu-repo/semantics/altIdentifier/pmid/7329210info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)reponame:SEDICI (UNLP)instname:Universidad Nacional de La Platainstacron:UNLP2024-05-08T13:10:50Zoai:sedici.unlp.edu.ar:10915/146070Institucionalhttp://sedici.unlp.edu.ar/Universidad públicaNo correspondehttp://sedici.unlp.edu.ar/oai/snrdalira@sedici.unlp.edu.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:13292024-05-08 13:10:50.493SEDICI (UNLP) - Universidad Nacional de La Platafalse
dc.title.none.fl_str_mv In vitro conversion of saturated to monounsaturated fatty acid by Ehrlich ascites cells
title In vitro conversion of saturated to monounsaturated fatty acid by Ehrlich ascites cells
spellingShingle In vitro conversion of saturated to monounsaturated fatty acid by Ehrlich ascites cells
Mercuri, Osvaldo Francisco
Bioquímica
Ehrlich ascites cells
Fatty acid
title_short In vitro conversion of saturated to monounsaturated fatty acid by Ehrlich ascites cells
title_full In vitro conversion of saturated to monounsaturated fatty acid by Ehrlich ascites cells
title_fullStr In vitro conversion of saturated to monounsaturated fatty acid by Ehrlich ascites cells
title_full_unstemmed In vitro conversion of saturated to monounsaturated fatty acid by Ehrlich ascites cells
title_sort In vitro conversion of saturated to monounsaturated fatty acid by Ehrlich ascites cells
dc.creator.none.fl_str_mv Mercuri, Osvaldo Francisco
De Tomás, María Elena
Antueno, Roberto Justo de
author Mercuri, Osvaldo Francisco
author_facet Mercuri, Osvaldo Francisco
De Tomás, María Elena
Antueno, Roberto Justo de
author_role author
author2 De Tomás, María Elena
Antueno, Roberto Justo de
author2_role author
author
dc.subject.none.fl_str_mv Bioquímica
Ehrlich ascites cells
Fatty acid
topic Bioquímica
Ehrlich ascites cells
Fatty acid
description In this paper, evidence is presented on the capacity of Ehrlich ascites cells to synthesize in vitro monounsaturated fatty acids from radioactive palmitate. Localization of the double bond was determined by ozonolysis and subsequent reduction of the ozonides to aldesters followed by gas liquid chromatography. These results proved that Ehrlich ascites cells have a Δ9 desaturase that catalyzes the biosynthesis of palmitoleic acid from palmitic acid and oleic and vaccenic acid via elongation-desaturation and desaturation-elongation, respectively, using palmitic acid as substrate. Furthermore, it is shown that, as in the hepatic cells, Δ9 desaturase enzyme activity of the tumoral cells is associated with the endoplasmic reticulum. The electron transport components involved in the desaturase system, i.e., NADH-cytochrome bs reductase and NADH-cytochrome bs reductase, were also measured. The activities of these enzymes do not appear to be rate-limiting in the desaturase activity of these tumoral cells.
publishDate 1981
dc.date.none.fl_str_mv 1981
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Articulo
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://sedici.unlp.edu.ar/handle/10915/146070
url http://sedici.unlp.edu.ar/handle/10915/146070
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/issn/0024-4201
info:eu-repo/semantics/altIdentifier/issn/1558-9307
info:eu-repo/semantics/altIdentifier/doi/10.1007/bf02534994
info:eu-repo/semantics/altIdentifier/pmid/7329210
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
dc.format.none.fl_str_mv application/pdf
893-896
dc.source.none.fl_str_mv reponame:SEDICI (UNLP)
instname:Universidad Nacional de La Plata
instacron:UNLP
instname_str Universidad Nacional de La Plata
instacron_str UNLP
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reponame_str SEDICI (UNLP)
collection SEDICI (UNLP)
repository.name.fl_str_mv SEDICI (UNLP) - Universidad Nacional de La Plata
repository.mail.fl_str_mv alira@sedici.unlp.edu.ar
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