Genetic diversity of Burkholderia contaminans isolates from cystic fibrosis patients in Argentina

A total of 120 Burkholderia cepacia complex isolates collected during 2004-2010 from 66 patients in two cystic fibrosis reference centers in Argentina were analyzed. Burkholderia contaminans was the species most frequently recovered (57.6%), followed by Burkholderia cenocepacia (15%), a species dist...

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Detalles Bibliográficos
Autores: Martina, Pablo Francisco, Bettiol, Marisa, Vescina, Cecilia, Montanaro, Patricia, Mannino, María Constanza, Prieto, Claudia Inés, Vay, Carlos, Naumann, Dieter, Schmitt, Juergen, Yantorno, Osvaldo Miguel, Lagares, Antonio, Bosch, María Alejandra
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2013
País:Argentina
Institución:Universidad Nacional de La Plata
Repositorio:SEDICI (UNLP)
Idioma:inglés
OAI Identifier:oai:sedici.unlp.edu.ar:10915/84971
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/84971
Access Level:acceso abierto
Palabra clave:Biología
Burkholderia
cystic fibrosis
BOXPCR genotyping
Descripción
Sumario:A total of 120 Burkholderia cepacia complex isolates collected during 2004-2010 from 66 patients in two cystic fibrosis reference centers in Argentina were analyzed. Burkholderia contaminans was the species most frequently recovered (57.6%), followed by Burkholderia cenocepacia (15%), a species distribution not reported so far. The recA-PCR-based techniques applied to the B. contaminans isolates revealed that 85% of the population carried the recA-ST-71 allele. Our results showed the utility of BOXPCR genotyping in analyzing B. contaminans diversity. This approach allowed us to address clonal transmission during an outbreak and the genetic changes occurring in infecting bacteria over the course of chronic infection.

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