Determination of phenolic compounds in strawberries (Fragaria ananassa Duch.) by high performance liquid chromatography with diode array detection

This paper describes a method for simultaneous identification and quantification of phenolic compounds in strawberries followed by a reversed-phase high performance liquid chromatography (HPLC) with diode array detection. The studied phenolics were flavonoids (flavonols: quercentin, rutin, and kaemp...

ver descrição completa

Detalhes bibliográficos
Autores: Tarola, Anna Maria, Van de Velde, Franco, Salvagni, Lucilla, Preti, Raffaella
Formato: artículo
Estado:Versión publicada
Fecha de publicación:2013
País:Argentina
Recursos:Consejo Nacional de Investigaciones Científicas y Técnicas
Repositorio:CONICET Digital (CONICET)
Idioma:inglés
OAI Identifier:oai:ri.conicet.gov.ar:11336/6422
Acesso em linha:http://hdl.handle.net/11336/6422
Access Level:acceso abierto
Palavra-chave:Strawberry Cultivars
Dad-Hplc
Anthocyanidins
Total Antioxidant Activity
https://purl.org/becyt/ford/1.4
https://purl.org/becyt/ford/1
id AR_2d5d6aef9de58c2ed83fcc2b831aa554
oai_identifier_str oai:ri.conicet.gov.ar:11336/6422
network_acronym_str AR
network_name_str Argentina
repository_id_str
spelling Determination of phenolic compounds in strawberries (Fragaria ananassa Duch.) by high performance liquid chromatography with diode array detectionTarola, Anna MariaVan de Velde, FrancoSalvagni, LucillaPreti, RaffaellaStrawberry CultivarsDad-HplcAnthocyanidinsTotal Antioxidant Activityhttps://purl.org/becyt/ford/1.4https://purl.org/becyt/ford/1This paper describes a method for simultaneous identification and quantification of phenolic compounds in strawberries followed by a reversed-phase high performance liquid chromatography (HPLC) with diode array detection. The studied phenolics were flavonoids (flavonols: quercentin, rutin, and kaempferol; flavanols: catechin and epicatechin; anthocyanidins: cyanidin and pelargonidin) and phenolic acids (hydroxybenzoic acid derivatives: gallic and ellagic acids; hydroxycinnamic acid derivatives: ferulic, coumaric, and cinnamic acids). The mobile phase consisted in a gradient prepared from formic acid in water (2 %, pH 3) and formic acid in methanol (2 %, pH 3), flow rate 0.7 mL min−1 at 25 °C. Analyses were performed, using methanol as extractant, before and after acid hydrolysis with the aim of determining free and conjugated phenolic compounds in strawberries. The acid hydrolysis conditions (6 mol L−1 HCl, 50 min at 90 °C) were shown to be suitable both for phenolic standards and strawberry extracts. Method validation, using phenolic standard solutions, included: linearity study, limits of detection and quantification, and calibration and analytical sensitivity quantifications. Precision and accuracy were studied in strawberry extracts. The results indicate that the developed method was linear, sensible, precise, and accurate, and the convenience of methanol can substitute acetonitrile as the most commonly used solvent in HPLC. The method was employed for knowing the phenolic profile in seven strawberry cultivars from Italy and Argentina, and besides, total phenolic content was analyzed by the Folin–Ciocalteu method; the total antioxidant activity was investigated using the ABTS method. Good correlations were observed among latter parameters, and total phenolics were obtained as the sum of each phenolic compound analyzed by photodiode array detection-HPLC.Fil: Tarola, Anna Maria. Sapienza Università di Roma; ItaliaFil: Van de Velde, Franco. Universidad Nacional del Litoral. Facultad de Ingeniería Química. Instituto de Tecnología de Los Alimentos; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Santa Fe; ArgentinaFil: Salvagni, Lucilla. Sapienza Università di Roma; ItaliaFil: Preti, Raffaella. Sapienza Università di Roma; ItaliaSpringer2013-02info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/6422Tarola, Anna Maria; Van de Velde, Franco; Salvagni, Lucilla; Preti, Raffaella; Determination of phenolic compounds in strawberries (Fragaria ananassa Duch.) by high performance liquid chromatography with diode array detection; Springer; Food Analytical Methods; 6; 1; 2-2013; 227-2371936-9751enginfo:eu-repo/semantics/altIdentifier/url/http://link.springer.com/article/10.1007/s12161-012-9431-5info:eu-repo/semantics/altIdentifier/doi/info:eu-repo/semantics/altIdentifier/doi/10.1007/s12161-012-9431-5info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2024-05-08T14:27:07Zoai:ri.conicet.gov.ar:11336/6422instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982024-05-08 14:27:07.758CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Determination of phenolic compounds in strawberries (Fragaria ananassa Duch.) by high performance liquid chromatography with diode array detection
title Determination of phenolic compounds in strawberries (Fragaria ananassa Duch.) by high performance liquid chromatography with diode array detection
spellingShingle Determination of phenolic compounds in strawberries (Fragaria ananassa Duch.) by high performance liquid chromatography with diode array detection
Tarola, Anna Maria
Strawberry Cultivars
Dad-Hplc
Anthocyanidins
Total Antioxidant Activity
https://purl.org/becyt/ford/1.4
https://purl.org/becyt/ford/1
title_short Determination of phenolic compounds in strawberries (Fragaria ananassa Duch.) by high performance liquid chromatography with diode array detection
title_full Determination of phenolic compounds in strawberries (Fragaria ananassa Duch.) by high performance liquid chromatography with diode array detection
title_fullStr Determination of phenolic compounds in strawberries (Fragaria ananassa Duch.) by high performance liquid chromatography with diode array detection
title_full_unstemmed Determination of phenolic compounds in strawberries (Fragaria ananassa Duch.) by high performance liquid chromatography with diode array detection
title_sort Determination of phenolic compounds in strawberries (Fragaria ananassa Duch.) by high performance liquid chromatography with diode array detection
dc.creator.none.fl_str_mv Tarola, Anna Maria
Van de Velde, Franco
Salvagni, Lucilla
Preti, Raffaella
author Tarola, Anna Maria
author_facet Tarola, Anna Maria
Van de Velde, Franco
Salvagni, Lucilla
Preti, Raffaella
author_role author
author2 Van de Velde, Franco
Salvagni, Lucilla
Preti, Raffaella
author2_role author
author
author
dc.subject.none.fl_str_mv Strawberry Cultivars
Dad-Hplc
Anthocyanidins
Total Antioxidant Activity
https://purl.org/becyt/ford/1.4
https://purl.org/becyt/ford/1
topic Strawberry Cultivars
Dad-Hplc
Anthocyanidins
Total Antioxidant Activity
https://purl.org/becyt/ford/1.4
https://purl.org/becyt/ford/1
description This paper describes a method for simultaneous identification and quantification of phenolic compounds in strawberries followed by a reversed-phase high performance liquid chromatography (HPLC) with diode array detection. The studied phenolics were flavonoids (flavonols: quercentin, rutin, and kaempferol; flavanols: catechin and epicatechin; anthocyanidins: cyanidin and pelargonidin) and phenolic acids (hydroxybenzoic acid derivatives: gallic and ellagic acids; hydroxycinnamic acid derivatives: ferulic, coumaric, and cinnamic acids). The mobile phase consisted in a gradient prepared from formic acid in water (2 %, pH 3) and formic acid in methanol (2 %, pH 3), flow rate 0.7 mL min−1 at 25 °C. Analyses were performed, using methanol as extractant, before and after acid hydrolysis with the aim of determining free and conjugated phenolic compounds in strawberries. The acid hydrolysis conditions (6 mol L−1 HCl, 50 min at 90 °C) were shown to be suitable both for phenolic standards and strawberry extracts. Method validation, using phenolic standard solutions, included: linearity study, limits of detection and quantification, and calibration and analytical sensitivity quantifications. Precision and accuracy were studied in strawberry extracts. The results indicate that the developed method was linear, sensible, precise, and accurate, and the convenience of methanol can substitute acetonitrile as the most commonly used solvent in HPLC. The method was employed for knowing the phenolic profile in seven strawberry cultivars from Italy and Argentina, and besides, total phenolic content was analyzed by the Folin–Ciocalteu method; the total antioxidant activity was investigated using the ABTS method. Good correlations were observed among latter parameters, and total phenolics were obtained as the sum of each phenolic compound analyzed by photodiode array detection-HPLC.
publishDate 2013
dc.date.none.fl_str_mv 2013-02
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/6422
Tarola, Anna Maria; Van de Velde, Franco; Salvagni, Lucilla; Preti, Raffaella; Determination of phenolic compounds in strawberries (Fragaria ananassa Duch.) by high performance liquid chromatography with diode array detection; Springer; Food Analytical Methods; 6; 1; 2-2013; 227-237
1936-9751
url http://hdl.handle.net/11336/6422
identifier_str_mv Tarola, Anna Maria; Van de Velde, Franco; Salvagni, Lucilla; Preti, Raffaella; Determination of phenolic compounds in strawberries (Fragaria ananassa Duch.) by high performance liquid chromatography with diode array detection; Springer; Food Analytical Methods; 6; 1; 2-2013; 227-237
1936-9751
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://link.springer.com/article/10.1007/s12161-012-9431-5
info:eu-repo/semantics/altIdentifier/doi/
info:eu-repo/semantics/altIdentifier/doi/10.1007/s12161-012-9431-5
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Springer
publisher.none.fl_str_mv Springer
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1799196422420037632
score 15,811543

Registos relacionados