Photodynamic Antibacterial Chemotherapy (PACT) Against Staphylococcus aureus and Escherichia coli Using Gold Nanoparticles and LED's Irradiation

Gold nanoparticles synthesized through sodium citrate (A-AuNPs) and sodium borohydrade (B-AuNPs) reduction of Au3+ and a photochemically initiated method (C-AuNPs) were tested for possible application in Photodynamic Antibacterial Chemotherapy. None of them presented inhibitory activity against bact...

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Detalles Bibliográficos
Autores: Silvero, María Jazmín, Arguello, Gerardo Aníbal, Becerra, María Cecilia
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2014
País:Argentina
Institución:Consejo Nacional de Investigaciones Científicas y Técnicas
Repositorio:CONICET Digital (CONICET)
Idioma:inglés
OAI Identifier:oai:ri.conicet.gov.ar:11336/22553
Acceso en línea:http://hdl.handle.net/11336/22553
Access Level:acceso abierto
Palabra clave:Gold Nanoparticles
Staphylococcus Aureus
Escherichia Coli
Photodynamic Antibacterial Chemoterapy
https://purl.org/becyt/ford/2.10
https://purl.org/becyt/ford/2
Descripción
Sumario:Gold nanoparticles synthesized through sodium citrate (A-AuNPs) and sodium borohydrade (B-AuNPs) reduction of Au3+ and a photochemically initiated method (C-AuNPs) were tested for possible application in Photodynamic Antibacterial Chemotherapy. None of them presented inhibitory activity against bacterial suspensions in dark conditions using the cylinders inhibition method; however, a total inhibition of Staphylococcus aureus ATCC 29213 and an extended-spectrum beta-lactamases-producing Escherichia coli ATCC 25922 growth were observed when incubated with 2 mM B-AuNPs and irradiated with LEDs at 525 nm for 12 h. Characterization of the interaction between S.aureus and B-AuNPs was demonstrated through an optimized procedure for TEM. These results probe that B-AuNPs have a good potential as photosensitizers for PACT.