Gradient elution LC-ESI-MS determination of cilostazol in rat plasma and its application

A sensitive and simple liquid chromatography/electrospray mass spectrometry (LC-ESI-MS) method for determination of cilostazol in rat plasma using one-step protein precipitation was developed. After addition of midazolam as internal standard (IS), protein precipitation by acetonitrile was used as sa...

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Detalles Bibliográficos
Autores: Wang, Jinjin, Wang, Zhibin, Wang, Zhiyi, Chen, Chan, Liu, Naihua, Ma, Jianshe, Wang, Xianqin, Hu, Lufeng
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2012
País:Argentina
Institución:Universidad Nacional de La Plata
Repositorio:SEDICI (UNLP)
Idioma:inglés
OAI Identifier:oai:sedici.unlp.edu.ar:10915/18097
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/18097
Access Level:acceso abierto
Palabra clave:Farmacia
cilostazol; gradient elution; LC-ESI-MS; rat plasma
cilostazo
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spelling Gradient elution LC-ESI-MS determination of cilostazol in rat plasma and its applicationWang, JinjinWang, ZhibinWang, ZhiyiChen, ChanLiu, NaihuaMa, JiansheWang, XianqinHu, LufengFarmaciacilostazol; gradient elution; LC-ESI-MS; rat plasmacilostazoA sensitive and simple liquid chromatography/electrospray mass spectrometry (LC-ESI-MS) method for determination of cilostazol in rat plasma using one-step protein precipitation was developed. After addition of midazolam as internal standard (IS), protein precipitation by acetonitrile was used as sample preparation. Chromatographic separation was achieved on an SB-C18 (2.1 × 50 mm, 5.0 μm) column with acetonitrile-0.1 % formic acid as mobile phase with gradient elution. Electrospray ionization (ESI) source was applied and operated in positive ion mode; selected ion monitoring (SIM) mode was used to quantification using target fragment ions m/z 370.0 for cilostazol and m/z 325.9 for the IS. Calibration plots were linear over the range of 10-2000 ng/mL for cilostazol in rat plasma. Lower limit of quantification (LLOQ) for cilostazol was 10 ng/mL. Mean recovery of cilostazol from plasma was in the range 90.14- 95.10 %. RSD of intra-day and inter-day precision were both less than 15 %. This method is simple and sensitive enough to be used in pharmacokinetic research for determination of cilostazol in rat plasma.Colegio de Farmacéuticos de la Provincia de Buenos Aires2012info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionArticulohttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdf240-244http://sedici.unlp.edu.ar/handle/10915/18097enginfo:eu-repo/semantics/altIdentifier/url/http://www.latamjpharm.org/resumenes/31/2/LAJOP_31_2_1_10.pdfinfo:eu-repo/semantics/openAccessLicencia de distribución no exclusiva SEDICIreponame:SEDICI (UNLP)instname:Universidad Nacional de La Platainstacron:UNLP2024-05-08T12:32:27Zoai:sedici.unlp.edu.ar:10915/18097Institucionalhttp://sedici.unlp.edu.ar/Universidad públicaNo correspondehttp://sedici.unlp.edu.ar/oai/snrdalira@sedici.unlp.edu.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:13292024-05-08 12:32:27.775SEDICI (UNLP) - Universidad Nacional de La Platafalse
dc.title.none.fl_str_mv Gradient elution LC-ESI-MS determination of cilostazol in rat plasma and its application
title Gradient elution LC-ESI-MS determination of cilostazol in rat plasma and its application
spellingShingle Gradient elution LC-ESI-MS determination of cilostazol in rat plasma and its application
Wang, Jinjin
Farmacia
cilostazol; gradient elution; LC-ESI-MS; rat plasma
cilostazo
title_short Gradient elution LC-ESI-MS determination of cilostazol in rat plasma and its application
title_full Gradient elution LC-ESI-MS determination of cilostazol in rat plasma and its application
title_fullStr Gradient elution LC-ESI-MS determination of cilostazol in rat plasma and its application
title_full_unstemmed Gradient elution LC-ESI-MS determination of cilostazol in rat plasma and its application
title_sort Gradient elution LC-ESI-MS determination of cilostazol in rat plasma and its application
dc.creator.none.fl_str_mv Wang, Jinjin
Wang, Zhibin
Wang, Zhiyi
Chen, Chan
Liu, Naihua
Ma, Jianshe
Wang, Xianqin
Hu, Lufeng
author Wang, Jinjin
author_facet Wang, Jinjin
Wang, Zhibin
Wang, Zhiyi
Chen, Chan
Liu, Naihua
Ma, Jianshe
Wang, Xianqin
Hu, Lufeng
author_role author
author2 Wang, Zhibin
Wang, Zhiyi
Chen, Chan
Liu, Naihua
Ma, Jianshe
Wang, Xianqin
Hu, Lufeng
author2_role author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Farmacia
cilostazol; gradient elution; LC-ESI-MS; rat plasma
cilostazo
topic Farmacia
cilostazol; gradient elution; LC-ESI-MS; rat plasma
cilostazo
description A sensitive and simple liquid chromatography/electrospray mass spectrometry (LC-ESI-MS) method for determination of cilostazol in rat plasma using one-step protein precipitation was developed. After addition of midazolam as internal standard (IS), protein precipitation by acetonitrile was used as sample preparation. Chromatographic separation was achieved on an SB-C18 (2.1 × 50 mm, 5.0 μm) column with acetonitrile-0.1 % formic acid as mobile phase with gradient elution. Electrospray ionization (ESI) source was applied and operated in positive ion mode; selected ion monitoring (SIM) mode was used to quantification using target fragment ions m/z 370.0 for cilostazol and m/z 325.9 for the IS. Calibration plots were linear over the range of 10-2000 ng/mL for cilostazol in rat plasma. Lower limit of quantification (LLOQ) for cilostazol was 10 ng/mL. Mean recovery of cilostazol from plasma was in the range 90.14- 95.10 %. RSD of intra-day and inter-day precision were both less than 15 %. This method is simple and sensitive enough to be used in pharmacokinetic research for determination of cilostazol in rat plasma.
publishDate 2012
dc.date.none.fl_str_mv 2012
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Articulo
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://sedici.unlp.edu.ar/handle/10915/18097
url http://sedici.unlp.edu.ar/handle/10915/18097
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://www.latamjpharm.org/resumenes/31/2/LAJOP_31_2_1_10.pdf
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
Licencia de distribución no exclusiva SEDICI
eu_rights_str_mv openAccess
rights_invalid_str_mv Licencia de distribución no exclusiva SEDICI
dc.format.none.fl_str_mv application/pdf
240-244
dc.source.none.fl_str_mv reponame:SEDICI (UNLP)
instname:Universidad Nacional de La Plata
instacron:UNLP
instname_str Universidad Nacional de La Plata
instacron_str UNLP
institution UNLP
reponame_str SEDICI (UNLP)
collection SEDICI (UNLP)
repository.name.fl_str_mv SEDICI (UNLP) - Universidad Nacional de La Plata
repository.mail.fl_str_mv alira@sedici.unlp.edu.ar
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score 15,811543

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