Multiplex PCR designed to differentiate C. glabrata complex species.

Background No phenotypic methods are available to unequivocally differentiate species within the Candida glabrata complex. Aims To develop a new multiplex PCR method to differentiate between the three species of the C. glabrata species complex, as well as using it to study a C. glabrata collection t...

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Detalles Bibliográficos
Autores: Dudiuk, Catiana Beatriz, Morales López, Soraya E., Podesta, Maria Virginia, Macedo, Daiana, Leonardelli, Florencia, Vitale, Roxana Gabriela, Tosello, Maria Elena Alejandra, Cabeza, Matías Sebastián, Biasoli, Marisa Susana, Gamarra, Soledad, Garcia, Guillermo Manuel
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2017
País:Argentina
Institución:Consejo Nacional de Investigaciones Científicas y Técnicas
Repositorio:CONICET Digital (CONICET)
Idioma:inglés
OAI Identifier:oai:ri.conicet.gov.ar:11336/178918
Acceso en línea:http://hdl.handle.net/11336/178918
Access Level:acceso abierto
Palabra clave:CANDIDA BRACARENSIS
CANDIDA GLABRATA COMPLEX
CANDIDA NIVARIENSIS
MOLECULAR IDENTIFICATION
https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
Descripción
Sumario:Background No phenotypic methods are available to unequivocally differentiate species within the Candida glabrata complex. Aims To develop a new multiplex PCR method to differentiate between the three species of the C. glabrata species complex, as well as using it to study a C. glabrata collection to discover strains of the newly described species. Methods The method was developed based on the Internal Transcribed Spacer (ITS) sequence differences between the species. It was validated by using a blinded collection of strains and, finally, the new molecular method was used to study a collection of 192 C. glabrata species complex strains. The obtained results were compared with ITS sequencing. Results The proposed method showed 100% concordance with ITS sequencing and proved to be effective for clinical and epidemiological applications. Two Candida bracarensis and three Candida nivariensis were found out of the 192 studied strains (0.93% and 1.40% prevalence, respectively). Conclusions A fast, inexpensive, robust and highly reproducible multiplex PCR method is presented. Its usefulness is demonstrated by studying a large collection of C. glabrata sensu lato strains.

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