Giant phospholipid vesicles: Comparison among the whole lipid sample characteristics using different preparation methods - A two photon fluorescence microscopy study

Several methods for the preparation of giant unilamellar vesicles (GUVs) using synthetic phosphatidylcholine phospholipids were evaluated. We compared the physical characteristics - in terms of lamellarity and morphology - of the whole lipid sample for each different lipid preparation using the sect...

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Autores: Bagatolli, Luis Alberto, Parasassi, T., Gratton, E.
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2000
País:Argentina
Institución:Consejo Nacional de Investigaciones Científicas y Técnicas
Repositorio:CONICET Digital (CONICET)
Idioma:inglés
OAI Identifier:oai:ri.conicet.gov.ar:11336/133918
Acceso en línea:http://hdl.handle.net/11336/133918
Access Level:acceso abierto
Palabra clave:6-LAUROYL-2-(N,N- DIMETHYLAMINO)NAPHTALENE
GENERALIZED POLARIZATION
GIANT UNILAMELLAR VESICLES
UNILAMELLAR
https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
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spelling Giant phospholipid vesicles: Comparison among the whole lipid sample characteristics using different preparation methods - A two photon fluorescence microscopy studyBagatolli, Luis AlbertoParasassi, T.Gratton, E.6-LAUROYL-2-(N,N- DIMETHYLAMINO)NAPHTALENEGENERALIZED POLARIZATIONGIANT UNILAMELLAR VESICLESUNILAMELLARhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Several methods for the preparation of giant unilamellar vesicles (GUVs) using synthetic phosphatidylcholine phospholipids were evaluated. We compared the physical characteristics - in terms of lamellarity and morphology - of the whole lipid sample for each different lipid preparation using the sectioning capability of the two-photon excitation fluorescence microscope. From the evaluation of the entire lipid sample we determined that vesicle size, internal shape and shell thickness distributions depend on the vesicle's preparation method. Our results show that the preparation of giant unilamellar vesicles by the application of external electric fields offers several advantages among the other methods tested here. Using this method a high yield (~95%) of giant unilamellar vesicles with a narrow size distribution was obtained. Independently of the preparation method, some lipid structures, which are held together by lipid tethers, were identified and resolved. These particular lipid structures show shell thickness and size heterogeneity. Labeling the lipid samples with 6-lauroyl-2-(N,N- dimethylamino)naphtalene (LAURDAN) and using the LAURDAN generalized polarization function we show that the lipid packing in these tethers or tubes is similar to those found in the phospholipid vesicles. The fact that both vesicles and tethers are found in the lipid preparations indicates similar stability between these structures.Fil: Bagatolli, Luis Alberto. University of Illinois. Urbana - Champaign; Estados UnidosFil: Parasassi, T.. Consiglio Nazionale delle Ricerche; Italia. Istituto di Medicina Sperimentale; ItaliaFil: Gratton, E.. University of Illinois. Urbana - Champaign; Estados UnidosElsevier Ireland2000-04info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/133918Bagatolli, Luis Alberto; Parasassi, T.; Gratton, E.; Giant phospholipid vesicles: Comparison among the whole lipid sample characteristics using different preparation methods - A two photon fluorescence microscopy study; Elsevier Ireland; Chemistry and Physics of Lipids; 105; 2; 4-2000; 135-1470009-3084CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://www.ncbi.nlm.nih.gov/pubmed/10823462info:eu-repo/semantics/altIdentifier/doi/10.1016/s0009-3084(00)00118-3info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2024-05-08T14:20:51Zoai:ri.conicet.gov.ar:11336/133918instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982024-05-08 14:20:51.607CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Giant phospholipid vesicles: Comparison among the whole lipid sample characteristics using different preparation methods - A two photon fluorescence microscopy study
title Giant phospholipid vesicles: Comparison among the whole lipid sample characteristics using different preparation methods - A two photon fluorescence microscopy study
spellingShingle Giant phospholipid vesicles: Comparison among the whole lipid sample characteristics using different preparation methods - A two photon fluorescence microscopy study
Bagatolli, Luis Alberto
6-LAUROYL-2-(N,N- DIMETHYLAMINO)NAPHTALENE
GENERALIZED POLARIZATION
GIANT UNILAMELLAR VESICLES
UNILAMELLAR
https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
title_short Giant phospholipid vesicles: Comparison among the whole lipid sample characteristics using different preparation methods - A two photon fluorescence microscopy study
title_full Giant phospholipid vesicles: Comparison among the whole lipid sample characteristics using different preparation methods - A two photon fluorescence microscopy study
title_fullStr Giant phospholipid vesicles: Comparison among the whole lipid sample characteristics using different preparation methods - A two photon fluorescence microscopy study
title_full_unstemmed Giant phospholipid vesicles: Comparison among the whole lipid sample characteristics using different preparation methods - A two photon fluorescence microscopy study
title_sort Giant phospholipid vesicles: Comparison among the whole lipid sample characteristics using different preparation methods - A two photon fluorescence microscopy study
dc.creator.none.fl_str_mv Bagatolli, Luis Alberto
Parasassi, T.
Gratton, E.
author Bagatolli, Luis Alberto
author_facet Bagatolli, Luis Alberto
Parasassi, T.
Gratton, E.
author_role author
author2 Parasassi, T.
Gratton, E.
author2_role author
author
dc.subject.none.fl_str_mv 6-LAUROYL-2-(N,N- DIMETHYLAMINO)NAPHTALENE
GENERALIZED POLARIZATION
GIANT UNILAMELLAR VESICLES
UNILAMELLAR
https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
topic 6-LAUROYL-2-(N,N- DIMETHYLAMINO)NAPHTALENE
GENERALIZED POLARIZATION
GIANT UNILAMELLAR VESICLES
UNILAMELLAR
https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
description Several methods for the preparation of giant unilamellar vesicles (GUVs) using synthetic phosphatidylcholine phospholipids were evaluated. We compared the physical characteristics - in terms of lamellarity and morphology - of the whole lipid sample for each different lipid preparation using the sectioning capability of the two-photon excitation fluorescence microscope. From the evaluation of the entire lipid sample we determined that vesicle size, internal shape and shell thickness distributions depend on the vesicle's preparation method. Our results show that the preparation of giant unilamellar vesicles by the application of external electric fields offers several advantages among the other methods tested here. Using this method a high yield (~95%) of giant unilamellar vesicles with a narrow size distribution was obtained. Independently of the preparation method, some lipid structures, which are held together by lipid tethers, were identified and resolved. These particular lipid structures show shell thickness and size heterogeneity. Labeling the lipid samples with 6-lauroyl-2-(N,N- dimethylamino)naphtalene (LAURDAN) and using the LAURDAN generalized polarization function we show that the lipid packing in these tethers or tubes is similar to those found in the phospholipid vesicles. The fact that both vesicles and tethers are found in the lipid preparations indicates similar stability between these structures.
publishDate 2000
dc.date.none.fl_str_mv 2000-04
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/133918
Bagatolli, Luis Alberto; Parasassi, T.; Gratton, E.; Giant phospholipid vesicles: Comparison among the whole lipid sample characteristics using different preparation methods - A two photon fluorescence microscopy study; Elsevier Ireland; Chemistry and Physics of Lipids; 105; 2; 4-2000; 135-147
0009-3084
CONICET Digital
CONICET
url http://hdl.handle.net/11336/133918
identifier_str_mv Bagatolli, Luis Alberto; Parasassi, T.; Gratton, E.; Giant phospholipid vesicles: Comparison among the whole lipid sample characteristics using different preparation methods - A two photon fluorescence microscopy study; Elsevier Ireland; Chemistry and Physics of Lipids; 105; 2; 4-2000; 135-147
0009-3084
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://www.ncbi.nlm.nih.gov/pubmed/10823462
info:eu-repo/semantics/altIdentifier/doi/10.1016/s0009-3084(00)00118-3
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier Ireland
publisher.none.fl_str_mv Elsevier Ireland
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 15.811543

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