Interfacial activation and bioimprinting of Candida rugosa lipase immobilized on polypropylene: Effect on the enzymatic activity in solvent-free ethyl oleate synthesis
Lipase from Candida rugosa adsorbed on polypropylene powder (CR/PP) was subjected to activation pre-treatments in order to enhance its activity in solvent-free ethyl oleate synthesis. The lipase activation achieved upon adsorption onto a hydrophobic support like PP was further enhanced through oil-w...
| Autores: | , , |
|---|---|
| Tipo de recurso: | artículo |
| Estado: | Versión publicada |
| Fecha de publicación: | 2005 |
| País: | Argentina |
| Institución: | Consejo Nacional de Investigaciones Científicas y Técnicas |
| Repositorio: | CONICET Digital (CONICET) |
| Idioma: | inglés |
| OAI Identifier: | oai:ri.conicet.gov.ar:11336/57696 |
| Acceso en línea: | http://hdl.handle.net/11336/57696 |
| Access Level: | acceso abierto |
| Palabra clave: | Adsorbed Lipase Bioimprinting Interfacial Activation https://purl.org/becyt/ford/2.9 https://purl.org/becyt/ford/2 |
| Sumario: | Lipase from Candida rugosa adsorbed on polypropylene powder (CR/PP) was subjected to activation pre-treatments in order to enhance its activity in solvent-free ethyl oleate synthesis. The lipase activation achieved upon adsorption onto a hydrophobic support like PP was further enhanced through oil-water interfacial activation and bioimprinting of the immobilized catalyst. Several aliphatic hydrocarbons/buffer pH 7 mixtures were used in the pre-activation of CR/PP with specific activity increments of up to 29%. Molecular bioimprinting was also performed, with specific activity enhancement of near 70% with respect to non-treated CR/PP. The effect of several fatty acids used as templates and the water present in the reaction medium was studied. The oil-water activation and bioimprinting treatments that led to the best activities were assayed at the immobilization step. Instead of pre-treating CR/PP adsorbed in buffer medium, interfacial activation with octane/buffer and bioimprinting with a mix of fatty acids were carried out in the immobilization vial. The best results were found for CR/PP immobilized in 5/95 octane/buffer (v/v, %) medium. In that way, a biocatalyst with enhanced specific activity is obtained right from the immobilization vial with no need of further activation steps prior to reaction. © 2004 Elsevier Inc. All rights reserved. |
|---|