Immunization with the Recombinant Cholera Toxin B Fused to Fimbria 2 Protein Protects against Bordetella pertussis Infection

This study examined the immunogenic properties of the fusion protein fimbria 2 ofBordetella pertussis(Fim2)—cholera toxin Bsubunit (CTB) in the intranasal murine model of infection. To this endB. pertussisFim2 coding sequence was cloned downstreamof the cholera toxin B subunit coding sequence. The e...

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Bibliographic Details
Authors: Olivera, Marianoelia, Castuma, Celina, Hozbor, Daniela Flavia, Gaillard, María Emilia, Rumbo, Martín, Gomez, Ricardo Martin
Format: article
Status:Published version
Publication Date:2014
Country:Argentina
Institution:Consejo Nacional de Investigaciones Científicas y Técnicas
Repository:CONICET Digital (CONICET)
Language:English
OAI Identifier:oai:ri.conicet.gov.ar:11336/32385
Online Access:http://hdl.handle.net/11336/32385
Access Level:Open access
Keyword:Bordetella
Vaccine
Fimbria
Protective Antigen
https://purl.org/becyt/ford/3.1
https://purl.org/becyt/ford/3
Description
Summary:This study examined the immunogenic properties of the fusion protein fimbria 2 ofBordetella pertussis(Fim2)—cholera toxin Bsubunit (CTB) in the intranasal murine model of infection. To this endB. pertussisFim2 coding sequence was cloned downstreamof the cholera toxin B subunit coding sequence. The expression and assembly of the fusion protein into pentameric structures (CTB-Fim2) were evaluated by SDS-PAGE and monosialotetrahexosylgaglioside (GM1-ganglioside) enzyme-linked immunosorbentassay (ELISA). To evaluate the protective capacity of CTB-Fim2, an intraperitoneal or intranasal mouse immunization schedulewas performed with 50휇g of CTB-Fim2. Recombinant (rFim2) or purified (BpFim2) Fim2, CTB, and phosphate-buffered saline(PBS) were used as controls. The results showed that mice immunized with BpFim2 or CTB-Fim2 intraperitoneally or intranasallypresented a significant reduction in bacterial lung counts compared to control groups (푃<0.01or푃<0.001, resp.). Moreover,intranasal immunization with CTB-Fim2 induced significant levels of Fim2-specific IgG in serum and bronchoalveolar lavage(BAL) and Fim2-specific IgA in BAL. Analysis of IgG isotypes and cytokines mRNA levels showed that CTB-Fim2 results in amixed Th1/Th2 (T-helper) response. The data presented here provide support for CTB-Fim2 as a promising recombinant antigenagainstBordetella pertussisinfection.

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