Application of a central composite design to the determination of inorganic and organic arsenic species in water by liquid chromatography-hydride generation-atomic absorption spectrometry
An on-line high performance liquid chromatography - hydride generation atomic absorption spectrometry coupled system (HPLC–HG–AAS) has been used for the determination of [As (III)], [As (V)], monomethylarsonic acid (MMA) and dimethylarsinic acid (DMA) in water samples. Four variables were taken into...
| Autores: | , , , |
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| Tipo de recurso: | artículo |
| Estado: | Versión publicada |
| Fecha de publicación: | 2009 |
| País: | Argentina |
| Institución: | Consejo Nacional de Investigaciones Científicas y Técnicas |
| Repositorio: | CONICET Digital (CONICET) |
| Idioma: | inglés |
| OAI Identifier: | oai:ri.conicet.gov.ar:11336/62118 |
| Acceso en línea: | http://hdl.handle.net/11336/62118 |
| Access Level: | acceso abierto |
| Palabra clave: | ARSENIC SPECIATION CENTRAL COMPOSITE DESIGN HIGH PERFORMANCE LIQUID CHROMATOGRAPHICS HYDRIDE GENERATION ATOMIC ABSORPTION SPECTROMETRY WATER ANALYSIS https://purl.org/becyt/ford/1.4 https://purl.org/becyt/ford/1 |
| Sumario: | An on-line high performance liquid chromatography - hydride generation atomic absorption spectrometry coupled system (HPLC–HG–AAS) has been used for the determination of [As (III)], [As (V)], monomethylarsonic acid (MMA) and dimethylarsinic acid (DMA) in water samples. Four variables were taken into account and a central composite design 24+* was used in the optimization of analytical conditions for the arsenic speciation method. The variables investigated were (A) mobile phase flow rate, (B) HCl concentration, (C) KI concentration, (D) NaBH4 concentration. Individual factors (A) and (B) were the most significant effects, and the optimum conditions for all factors were A = 1.2 mL min–1, B = 2.8 mol L–1, C = 1.7 % w/v, and D = 1.4 % w/v. The repeatability, stability, calibration curve and limit of detection were established. The method was applied to different kinds of waters; good recoveries of added spikes (92 – 109 %) were obtained for all analytes. |
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