Analysis Of Bacterial Chemotactic Response Using Dynamic Laser Speckle
Chemotaxis has a meaningful role in several fields, such as microbial physiology, medicine and biotechnology. We present a new application of dynamic laser speckle or biospeckle to detect different degrees of bacterial motility during chemotactic response experiments. Encouraging results showed diff...
| Autores: | , , , , , , |
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| Tipo de recurso: | artículo |
| Estado: | Versión publicada |
| Fecha de publicación: | 2009 |
| País: | Argentina |
| Institución: | Comisión de Investigaciones Científicas de la Provincia de Buenos Aires |
| Repositorio: | CIC Digital (CICBA) |
| Idioma: | inglés |
| OAI Identifier: | oai:digital.cic.gba.gob.ar:11746/10628 |
| Acceso en línea: | https://digital.cic.gba.gob.ar/handle/11746/10628 |
| Access Level: | acceso abierto |
| Palabra clave: | Óptica, Acústica Ciencias Físicas Dynamic speckle Chemotaxis Bacteria Biospeckle |
| Sumario: | Chemotaxis has a meaningful role in several fields, such as microbial physiology, medicine and biotechnology. We present a new application of dynamic laser speckle or biospeckle to detect different degrees of bacterial motility during chemotactic response experiments. Encouraging results showed different bacterial dynamic responses due to differences in the hardness of the support in the swarming plates. We compare this method to a conventional technique that uses white light. Both methods showed to be analogous and, in some cases, complementary. The results suggest that biospeckle processed images can be used as an alternative method to evaluate bacterial chemotactic response and can supply additional information about the bacterial motility in different areas of the swarm plate assay that might be useful for biological analysis. |
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