Gsk-3 β inhibitors mimic the cardioprotection mediated by ischemic pre- and postconditioning in hypertensive rats
The aim of this study was to examine the effects of GSK-3 inhibitors compared with PRE and POS in spontaneously hypertensive rats (SHR). Isolated hearts were submitted to the following protocols: IC: 45 min global ischemia (GI) and 1-hour reperfusion (R); PRE: a cycle of 5 min GI and 10 minutes of R...
| Autores: | , , |
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| Tipo de recurso: | artículo |
| Estado: | Versión publicada |
| Fecha de publicación: | 2013 |
| País: | Argentina |
| Institución: | Consejo Nacional de Investigaciones Científicas y Técnicas |
| Repositorio: | CONICET Digital (CONICET) |
| Idioma: | inglés |
| OAI Identifier: | oai:ri.conicet.gov.ar:11336/11946 |
| Acceso en línea: | http://hdl.handle.net/11336/11946 |
| Access Level: | acceso abierto |
| Palabra clave: | Gsk-3 Beta Ischemic Preconditioning Ischemic Postconditioning Mitochondrial Swelling https://purl.org/becyt/ford/3.1 https://purl.org/becyt/ford/3 |
| Sumario: | The aim of this study was to examine the effects of GSK-3 inhibitors compared with PRE and POS in spontaneously hypertensive rats (SHR). Isolated hearts were submitted to the following protocols: IC: 45 min global ischemia (GI) and 1-hour reperfusion (R); PRE: a cycle of 5 min GI and 10 minutes of R prior to 45 min GI; POS: three cycles of 30 sec GI/30 sec R at the start of R. Other hearts received lithium chloride (LiCl) or indirubin-3 -monoxime,5-iodo-(IMI) as GSK-3 inhibitors. All interventions reduced the infarct size observed in IC group. The expressions of P-GSK-3 and P-Akt decreased in IC and were restored after PRE, POS, and GSK-3 inhibitors treatments. An increase of cytosolic MnSOD activity and lipid peroxidation and a decrease of GSH content observed in IC hearts were attenuated in PRE, POS, and LiCl or IMI treatments. An increase of P-GSK-3/VDAC physical association and a partial recovery of mitochondrial permeability were also detected after interventions. These data show that, in SHR hearts, GSK-3 inhibitors mimic the cardioprotection afforded by PRE and POS and suggest that a decrease in mitochondrial permeability mediated by P-GSK-3/VDAC interaction is a crucial event. |
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