Potato Snakin-1 gene silencing affects cell division, primary metabolism, and cell wall composition

Snakin-1 (SN1) is an antimicrobial cysteine-rich peptide isolated from potato (Solanum tuberosum) that was classified as a member of the Snakin/Gibberellic Acid Stimulated in Arabidopsis protein family. In this work, a transgenic approach was used to study the role of SN1 in planta. Even when overex...

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Detalles Bibliográficos
Autores: Nahirñak, V., Almasia, N.I., Fernandez, P.V., Hopp, H.E., Estevez, J.M., Carrari, F., Vazquez-Rovere, C.
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2012
País:Argentina
Institución:Universidad Nacional de Buenos Aires. Facultad de Ciencias Exactas y Naturales
Repositorio:Biblioteca Digital (UBA-FCEN)
Idioma:inglés
OAI Identifier:paperaa:paper_00320889_v158_n1_p252_Nahirnak
Acceso en línea:http://hdl.handle.net/20.500.12110/paper_00320889_v158_n1_p252_Nahirnak
Access Level:acceso abierto
Palabra clave:green fluorescent protein
SN1 protein, Solanum tuberosum
vegetable protein
article
cell division
cell membrane
cell wall
chemistry
cytology
gene expression regulation
gene silencing
genetics
infrared spectroscopy
mass fragmentography
metabolism
molecular genetics
nucleotide sequence
physiology
plant epidermis
plant leaf
potato
Solanaceae
transgenic plant
Cell Division
Cell Membrane
Cell Wall
Gas Chromatography-Mass Spectrometry
Gene Expression Regulation, Plant
Gene Silencing
Green Fluorescent Proteins
Molecular Sequence Data
Plant Epidermis
Plant Leaves
Plant Proteins
Plants, Genetically Modified
Solanum tuberosum
Spectroscopy, Fourier Transform Infrared
Arabidopsis
Nicotiana benthamiana
Descripción
Sumario:Snakin-1 (SN1) is an antimicrobial cysteine-rich peptide isolated from potato (Solanum tuberosum) that was classified as a member of the Snakin/Gibberellic Acid Stimulated in Arabidopsis protein family. In this work, a transgenic approach was used to study the role of SN1 in planta. Even when overexpressing SN1, potato lines did not show remarkable morphological differences from the wild type; SN1 silencing resulted in reduced height, which was accompanied by an overall reduction in leaf size and severe alterations of leaf shape. Analysis of the adaxial epidermis of mature leaves revealed that silenced lines had 70% to 90% increases in mean cell size with respect to wild-type leaves. Consequently, the number of epidermal cells was significantly reduced in these lines. Confocal microscopy analysis after agroinfiltration of Nicotiana benthamiana leaves showed that SN1-green fluorescent protein fusion protein was localized in plasma membrane, and bimolecular fluorescence complementation assays revealed that SN1 self-interacted in vivo. We further focused our study on leaf metabolism by applying a combination of gas chromatography coupled to mass spectrometry, Fourier transform infrared spectroscopy, and spectrophotometric techniques. These targeted analyses allowed a detailed examination of the changes occurring in 46 intermediate compounds from primary metabolic pathways and in seven cell wall constituents. We demonstrated that SN1 silencing affects cell division, leaf primary metabolism, and cell wall composition in potato plants, suggesting that SN1 has additional roles in growth and development beyond its previously assigned role in plant defense. © 2011 American Society of Plant Biologists. All Rights Reserved.