β-adrenergic stimulation controls the expression of a thioesterase specific for very-long-chain fatty acids in perfused hearts
Arachidonic acid is not freely stored in the cells. A number of different pathways for the mobilization of this compound have been proposed, including a novel mechanism that involves the release of arachidonic acid from arachidonoyl-CoA by a thioesterase with substrate specificity for very-long-chai...
| Autores: | , , , , , , |
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| Tipo de recurso: | artículo |
| Estado: | Versión publicada |
| Fecha de publicación: | 2002 |
| País: | Argentina |
| Institución: | Consejo Nacional de Investigaciones Científicas y Técnicas |
| Repositorio: | CONICET Digital (CONICET) |
| Idioma: | inglés |
| OAI Identifier: | oai:ri.conicet.gov.ar:11336/171931 |
| Acceso en línea: | http://hdl.handle.net/11336/171931 |
| Access Level: | acceso abierto |
| Palabra clave: | ACYL-COA THIOESTERASE ARACHIDONIC ACID CARDIAC TISSUE HEART PERFUSION ISOPROTERENOL MRNA LEVELS https://purl.org/becyt/ford/3.1 https://purl.org/becyt/ford/3 |
| Sumario: | Arachidonic acid is not freely stored in the cells. A number of different pathways for the mobilization of this compound have been proposed, including a novel mechanism that involves the release of arachidonic acid from arachidonoyl-CoA by a thioesterase with substrate specificity for very-long-chain fatty acids. In rat heart, the acyl-CoA thioesterase activity can be regulated by a mechanism that involves β-adrenoceptors. In this paper we demonstrate that β-adrenergic agonists also regulate the acyl-CoA thioesterase mRNA levels. Isoproterenol (10-7) a concentration known to exert physiological responses - increases in a time-dependent manner the acyl-CoA thioesterase mRNA levels, an effect blocked by a specific β-adrenoceptor antagonist. In addition, our results show that cAMP is involved in this process. The acyl-CoA thioesterase mRNA levels are also increased by fasting, but not by di(2-ethylhexyl)phthalate, a peroxisome proliferator. These results may suggest the existence of a β-adrenoceptor-activated regulatory pathway for arachidonic acid release in cardiac tissue. © 2002 Elsevier Science (USA). All rights reserved. |
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