Glycoconjugate histochemistry in the small and large intestine of normal and Solanum glaucophyllum-intoxicated rabbits

Vitamin D participates in mineral homeostasis, immunomodulation, cell growth and differentiation. The leaves of Solanum glaucophyllum contain high levels of 1,25-dihydroxyvitamin D3 as glycoside derivatives and their chronic ingestion generates a hypervitaminosis D-like state. We analyzed changes on...

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Detalles Bibliográficos
Autores: Zanuzzi, Carolina Natalia, Barbeito, Claudio Gustavo, Ortíz, M. L., Fontana, Paula Andrea, Portiansky, Enrique Leo, Gimeno, Eduardo Juan
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2010
País:Argentina
Institución:Consejo Nacional de Investigaciones Científicas y Técnicas
Repositorio:CONICET Digital (CONICET)
Idioma:inglés
OAI Identifier:oai:ri.conicet.gov.ar:11336/125549
Acceso en línea:http://hdl.handle.net/11336/125549
Access Level:acceso abierto
Palabra clave:DIFFERENTIATION
GLYCOSYLATION
HISTOCHEMISTRY
INTESTINE
LECTINS
VITAMIN D3
https://purl.org/becyt/ford/4.3
https://purl.org/becyt/ford/4
Descripción
Sumario:Vitamin D participates in mineral homeostasis, immunomodulation, cell growth and differentiation. The leaves of Solanum glaucophyllum contain high levels of 1,25-dihydroxyvitamin D3 as glycoside derivatives and their chronic ingestion generates a hypervitaminosis D-like state. We analyzed changes on carbohydrate expression as a cell differentiation indicator on samples of the small and large intestine of S. glaucophyllum-intoxicated rabbits, using conventional and lectin histochemistry. Male New Zealand white rabbits were intoxicated with S. glaucophyllum during two or four weeks and killed the day after. A group of animals (" possibly recovered group" ) were intoxicated during 15. days and killed at day 45 of the beginning of the experiment. We found changes in the lectin binding pattern in the small and large intestine of the intoxicated rabbits. Some of these changes were reverted in the possibly recovered group. Vitamin D could be a new regulator factor of the intestinal glycosylation process.