Chronic treatment with atrial natriuretic Peptide in spontaneously hypertensive rats: beneficial renal effects and sex differences.

OBJECTIVE: The aim of this study was to investigate the effects of chronic treatment with atrial natriuretic peptide (ANP) on renal function, nitric oxide (NO) system, oxidative stress, collagen content and apoptosis in kidneys of spontaneously hypertensive rats (SHR), as well as sex-related differe...

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Autores: Romero, Mariana, Caniffi, Carolina Cecilia, Bouchet, Gonzalo, Costa, Maria de Los Angeles, Elesgaray, Rosana, Arranz, Cristina Teresa, Tomat, Analia Lorena
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2015
País:Argentina
Institución:Consejo Nacional de Investigaciones Científicas y Técnicas
Repositorio:CONICET Digital (CONICET)
Idioma:inglés
OAI Identifier:oai:ri.conicet.gov.ar:11336/18383
Acceso en línea:http://hdl.handle.net/11336/18383
Access Level:acceso abierto
Palabra clave:Hypertension
Atrial Natriuretic peptide
Kidney
Nitric oxide
https://purl.org/becyt/ford/3.1
https://purl.org/becyt/ford/3
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network_acronym_str AR
network_name_str Argentina
repository_id_str
dc.title.none.fl_str_mv Chronic treatment with atrial natriuretic Peptide in spontaneously hypertensive rats: beneficial renal effects and sex differences.
title Chronic treatment with atrial natriuretic Peptide in spontaneously hypertensive rats: beneficial renal effects and sex differences.
spellingShingle Chronic treatment with atrial natriuretic Peptide in spontaneously hypertensive rats: beneficial renal effects and sex differences.
Romero, Mariana
Hypertension
Atrial Natriuretic peptide
Kidney
Nitric oxide
https://purl.org/becyt/ford/3.1
https://purl.org/becyt/ford/3
title_short Chronic treatment with atrial natriuretic Peptide in spontaneously hypertensive rats: beneficial renal effects and sex differences.
title_full Chronic treatment with atrial natriuretic Peptide in spontaneously hypertensive rats: beneficial renal effects and sex differences.
title_fullStr Chronic treatment with atrial natriuretic Peptide in spontaneously hypertensive rats: beneficial renal effects and sex differences.
title_full_unstemmed Chronic treatment with atrial natriuretic Peptide in spontaneously hypertensive rats: beneficial renal effects and sex differences.
title_sort Chronic treatment with atrial natriuretic Peptide in spontaneously hypertensive rats: beneficial renal effects and sex differences.
dc.creator.none.fl_str_mv Romero, Mariana
Caniffi, Carolina Cecilia
Bouchet, Gonzalo
Costa, Maria de Los Angeles
Elesgaray, Rosana
Arranz, Cristina Teresa
Tomat, Analia Lorena
author Romero, Mariana
author_facet Romero, Mariana
Caniffi, Carolina Cecilia
Bouchet, Gonzalo
Costa, Maria de Los Angeles
Elesgaray, Rosana
Arranz, Cristina Teresa
Tomat, Analia Lorena
author_role author
author2 Caniffi, Carolina Cecilia
Bouchet, Gonzalo
Costa, Maria de Los Angeles
Elesgaray, Rosana
Arranz, Cristina Teresa
Tomat, Analia Lorena
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv Hypertension
Atrial Natriuretic peptide
Kidney
Nitric oxide
https://purl.org/becyt/ford/3.1
https://purl.org/becyt/ford/3
topic Hypertension
Atrial Natriuretic peptide
Kidney
Nitric oxide
https://purl.org/becyt/ford/3.1
https://purl.org/becyt/ford/3
description OBJECTIVE: The aim of this study was to investigate the effects of chronic treatment with atrial natriuretic peptide (ANP) on renal function, nitric oxide (NO) system, oxidative stress, collagen content and apoptosis in kidneys of spontaneously hypertensive rats (SHR), as well as sex-related differences in the response to the treatment. METHODS: 10 week-old male and female SHR were infused with ANP (100 ng/h/rat) or saline (NaCl 0.9%) for 14 days (subcutaneous osmotic pumps). Systolic blood pressure (SBP) was recorded and diuresis and natriuresis were determined. After treatment, renal NO synthase (NOS) activity and eNOS expression were evaluated. Thiobarbituric acid-reactive substances (TBARS), glutathione concentration and glutathione peroxidase (GPx) and superoxide dismutase (SOD) activities were determined in the kidney. Collagen was identified in renal slices by Sirius red staining and apoptosis by Tunel assay. RESULTS: Female SHR showed lower SBP, oxidative stress, collagen content and apoptosis in kidney, and higher renal NOS activity and eNOS protein content, than males. ANP lowered SBP, increased diuresis, natriuresis, renal NOS activity and eNOS expression in both sexes. Renal response to ANP was more marked in females than in males. In kidney, ANP reduced TBARS, renal collagen content and apoptosis, and increased glutathione concentration and activity of GPx and SOD enzymes in both sexes. CONCLUSIONS: Female SHR exhibited less organ damage than males. Chronic ANP treatment would ameliorate hypertension and end-organ damage in the kidney by reducing oxidative stress, increasing NO-system activity, and diminishing collagen content and apoptosis, in both sexes.
publishDate 2015
dc.date.none.fl_str_mv 2015-03
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/18383
Romero, Mariana; Caniffi, Carolina Cecilia; Bouchet, Gonzalo; Costa, Maria de Los Angeles; Elesgaray, Rosana; et al.; Chronic treatment with atrial natriuretic Peptide in spontaneously hypertensive rats: beneficial renal effects and sex differences.; Public Library Of Science; Plos One; 10; 3; 3-2015; 1-13; e0120362
1932-6203
CONICET Digital
CONICET
url http://hdl.handle.net/11336/18383
identifier_str_mv Romero, Mariana; Caniffi, Carolina Cecilia; Bouchet, Gonzalo; Costa, Maria de Los Angeles; Elesgaray, Rosana; et al.; Chronic treatment with atrial natriuretic Peptide in spontaneously hypertensive rats: beneficial renal effects and sex differences.; Public Library Of Science; Plos One; 10; 3; 3-2015; 1-13; e0120362
1932-6203
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1371/journal.pone.0120362
info:eu-repo/semantics/altIdentifier/ark/http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0120362
info:eu-repo/semantics/altIdentifier/url/https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4361555/
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
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dc.publisher.none.fl_str_mv Public Library Of Science
publisher.none.fl_str_mv Public Library Of Science
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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spelling Chronic treatment with atrial natriuretic Peptide in spontaneously hypertensive rats: beneficial renal effects and sex differences.Romero, MarianaCaniffi, Carolina CeciliaBouchet, GonzaloCosta, Maria de Los AngelesElesgaray, RosanaArranz, Cristina TeresaTomat, Analia LorenaHypertensionAtrial Natriuretic peptideKidneyNitric oxidehttps://purl.org/becyt/ford/3.1https://purl.org/becyt/ford/3OBJECTIVE: The aim of this study was to investigate the effects of chronic treatment with atrial natriuretic peptide (ANP) on renal function, nitric oxide (NO) system, oxidative stress, collagen content and apoptosis in kidneys of spontaneously hypertensive rats (SHR), as well as sex-related differences in the response to the treatment. METHODS: 10 week-old male and female SHR were infused with ANP (100 ng/h/rat) or saline (NaCl 0.9%) for 14 days (subcutaneous osmotic pumps). Systolic blood pressure (SBP) was recorded and diuresis and natriuresis were determined. After treatment, renal NO synthase (NOS) activity and eNOS expression were evaluated. Thiobarbituric acid-reactive substances (TBARS), glutathione concentration and glutathione peroxidase (GPx) and superoxide dismutase (SOD) activities were determined in the kidney. Collagen was identified in renal slices by Sirius red staining and apoptosis by Tunel assay. RESULTS: Female SHR showed lower SBP, oxidative stress, collagen content and apoptosis in kidney, and higher renal NOS activity and eNOS protein content, than males. ANP lowered SBP, increased diuresis, natriuresis, renal NOS activity and eNOS expression in both sexes. Renal response to ANP was more marked in females than in males. In kidney, ANP reduced TBARS, renal collagen content and apoptosis, and increased glutathione concentration and activity of GPx and SOD enzymes in both sexes. CONCLUSIONS: Female SHR exhibited less organ damage than males. Chronic ANP treatment would ameliorate hypertension and end-organ damage in the kidney by reducing oxidative stress, increasing NO-system activity, and diminishing collagen content and apoptosis, in both sexes.Fil: Romero, Mariana. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Química y Metabolismo del Fármaco. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Química y Metabolismo del Fármaco; Argentina. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; ArgentinaFil: Caniffi, Carolina Cecilia. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Química y Metabolismo del Fármaco. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Química y Metabolismo del Fármaco; Argentina. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; ArgentinaFil: Bouchet, Gonzalo. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Química y Metabolismo del Fármaco. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Química y Metabolismo del Fármaco; ArgentinaFil: Costa, Maria de Los Angeles. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Química y Metabolismo del Fármaco. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Química y Metabolismo del Fármaco; ArgentinaFil: Elesgaray, Rosana. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Química y Metabolismo del Fármaco. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Química y Metabolismo del Fármaco; Argentina. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; ArgentinaFil: Arranz, Cristina Teresa. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Química y Metabolismo del Fármaco. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Química y Metabolismo del Fármaco; Argentina. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; ArgentinaFil: Tomat, Analia Lorena. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Química y Metabolismo del Fármaco. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Química y Metabolismo del Fármaco; Argentina. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; ArgentinaPublic Library Of Science2015-03info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/18383Romero, Mariana; Caniffi, Carolina Cecilia; Bouchet, Gonzalo; Costa, Maria de Los Angeles; Elesgaray, Rosana; et al.; Chronic treatment with atrial natriuretic Peptide in spontaneously hypertensive rats: beneficial renal effects and sex differences.; Public Library Of Science; Plos One; 10; 3; 3-2015; 1-13; e01203621932-6203CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1371/journal.pone.0120362info:eu-repo/semantics/altIdentifier/ark/http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0120362info:eu-repo/semantics/altIdentifier/url/https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4361555/info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2024-05-08T13:32:40Zoai:ri.conicet.gov.ar:11336/18383instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982024-05-08 13:32:40.838CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
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