Treatment with the synthetic PPARG ligand pioglitazone ameliorates early ovarian alterations induced by dehydroepiandrosterone in prepubertal rats

Background: Peroxisome proliferator-activated receptor gamma (PPARG) is a nuclear factor that may act on the early development of ovarian follicles and on follicular steroidogenesis. However, the exact mechanism of PPARG action remains unknown. We have previously found that androgen excess alters ea...

Descripción completa

Detalles Bibliográficos
Autores: Velez, Leandro Martin, Abruzzese, Giselle Adriana, Heber, María Florencia, Ferreira, Silvana Rocio, Motta, Alicia Beatriz
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2019
País:Argentina
Institución:Consejo Nacional de Investigaciones Científicas y Técnicas
Repositorio:CONICET Digital (CONICET)
Idioma:inglés
OAI Identifier:oai:ri.conicet.gov.ar:11336/120010
Acceso en línea:http://hdl.handle.net/11336/120010
Access Level:acceso abierto
Palabra clave:DEHYDROEPIANDROSTERONE
FOLLICULOGENESIS
OVARIAN FUNCTION
PIOGLITAZONE
PPARG
https://purl.org/becyt/ford/3.5
https://purl.org/becyt/ford/3
id AR_13033f10c375e51ced72d00a5d075014
oai_identifier_str oai:ri.conicet.gov.ar:11336/120010
network_acronym_str AR
network_name_str Argentina
repository_id_str
dc.title.none.fl_str_mv Treatment with the synthetic PPARG ligand pioglitazone ameliorates early ovarian alterations induced by dehydroepiandrosterone in prepubertal rats
title Treatment with the synthetic PPARG ligand pioglitazone ameliorates early ovarian alterations induced by dehydroepiandrosterone in prepubertal rats
spellingShingle Treatment with the synthetic PPARG ligand pioglitazone ameliorates early ovarian alterations induced by dehydroepiandrosterone in prepubertal rats
Velez, Leandro Martin
DEHYDROEPIANDROSTERONE
FOLLICULOGENESIS
OVARIAN FUNCTION
PIOGLITAZONE
PPARG
https://purl.org/becyt/ford/3.5
https://purl.org/becyt/ford/3
title_short Treatment with the synthetic PPARG ligand pioglitazone ameliorates early ovarian alterations induced by dehydroepiandrosterone in prepubertal rats
title_full Treatment with the synthetic PPARG ligand pioglitazone ameliorates early ovarian alterations induced by dehydroepiandrosterone in prepubertal rats
title_fullStr Treatment with the synthetic PPARG ligand pioglitazone ameliorates early ovarian alterations induced by dehydroepiandrosterone in prepubertal rats
title_full_unstemmed Treatment with the synthetic PPARG ligand pioglitazone ameliorates early ovarian alterations induced by dehydroepiandrosterone in prepubertal rats
title_sort Treatment with the synthetic PPARG ligand pioglitazone ameliorates early ovarian alterations induced by dehydroepiandrosterone in prepubertal rats
dc.creator.none.fl_str_mv Velez, Leandro Martin
Abruzzese, Giselle Adriana
Heber, María Florencia
Ferreira, Silvana Rocio
Motta, Alicia Beatriz
author Velez, Leandro Martin
author_facet Velez, Leandro Martin
Abruzzese, Giselle Adriana
Heber, María Florencia
Ferreira, Silvana Rocio
Motta, Alicia Beatriz
author_role author
author2 Abruzzese, Giselle Adriana
Heber, María Florencia
Ferreira, Silvana Rocio
Motta, Alicia Beatriz
author2_role author
author
author
author
dc.subject.none.fl_str_mv DEHYDROEPIANDROSTERONE
FOLLICULOGENESIS
OVARIAN FUNCTION
PIOGLITAZONE
PPARG
https://purl.org/becyt/ford/3.5
https://purl.org/becyt/ford/3
topic DEHYDROEPIANDROSTERONE
FOLLICULOGENESIS
OVARIAN FUNCTION
PIOGLITAZONE
PPARG
https://purl.org/becyt/ford/3.5
https://purl.org/becyt/ford/3
description Background: Peroxisome proliferator-activated receptor gamma (PPARG) is a nuclear factor that may act on the early development of ovarian follicles and on follicular steroidogenesis. However, the exact mechanism of PPARG action remains unknown. We have previously found that androgen excess alters early ovarian function and the PPARG system. The aim of the present study was to evaluate whether PPARG activation (using the synthetic ligand pioglitazone (PGZ)) ameliorates the alterations in early ovarian function induced by androgen excess. Methods: Female prepubertal rats were treated with equine chorionic gonadotropin (eCG) to induce folliculogenesis, together with dehydroepiandrosterone (DHEA) to induce hyperandrogenism and/or PGZ to evaluate PPARG activation. We assessed i) very early ovarian folliculogenesis, ii) PPARG activation, iii) ovarian steroidogenic enzymes, iv) the estradiol/testosterone ratio, v) the ovarian inflammatory status and vi) oxidative stress. Results: PGZ prevented the inactivation of ovarian PPARG induced by androgen excess by increasing PPARG itself and the gene expression of PPARG-coactivator 1 alpha (PGC1A), and by decreasing the gene expression of nuclear co-repressor (NCOR). PGZ also prevented the altered ovarian steroidogenesis, pro-inflammatory status and oxidative stress induced by androgen excess. Conclusions: Our findings suggest that PPARG activation plays important roles in modulating early ovarian function, and highlight the importance of understanding the role(s) of PPARG activation in the ovary, and the possible involvement in the treatment of ovarian pathologies, and/or the impact in regulating/improving fertility.
publishDate 2019
dc.date.none.fl_str_mv 2019-02
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/120010
Velez, Leandro Martin; Abruzzese, Giselle Adriana; Heber, María Florencia; Ferreira, Silvana Rocio; Motta, Alicia Beatriz; Treatment with the synthetic PPARG ligand pioglitazone ameliorates early ovarian alterations induced by dehydroepiandrosterone in prepubertal rats; Polish Acad Sciences Inst Pharmacology; Pharmacological Reports; 71; 1; 2-2019; 96-104
1734-1140
CONICET Digital
CONICET
url http://hdl.handle.net/11336/120010
identifier_str_mv Velez, Leandro Martin; Abruzzese, Giselle Adriana; Heber, María Florencia; Ferreira, Silvana Rocio; Motta, Alicia Beatriz; Treatment with the synthetic PPARG ligand pioglitazone ameliorates early ovarian alterations induced by dehydroepiandrosterone in prepubertal rats; Polish Acad Sciences Inst Pharmacology; Pharmacological Reports; 71; 1; 2-2019; 96-104
1734-1140
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S1734114018301981?via%3Dihub
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.pharep.2018.09.009
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Polish Acad Sciences Inst Pharmacology
publisher.none.fl_str_mv Polish Acad Sciences Inst Pharmacology
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1799194759692025856
spelling Treatment with the synthetic PPARG ligand pioglitazone ameliorates early ovarian alterations induced by dehydroepiandrosterone in prepubertal ratsVelez, Leandro MartinAbruzzese, Giselle AdrianaHeber, María FlorenciaFerreira, Silvana RocioMotta, Alicia BeatrizDEHYDROEPIANDROSTERONEFOLLICULOGENESISOVARIAN FUNCTIONPIOGLITAZONEPPARGhttps://purl.org/becyt/ford/3.5https://purl.org/becyt/ford/3Background: Peroxisome proliferator-activated receptor gamma (PPARG) is a nuclear factor that may act on the early development of ovarian follicles and on follicular steroidogenesis. However, the exact mechanism of PPARG action remains unknown. We have previously found that androgen excess alters early ovarian function and the PPARG system. The aim of the present study was to evaluate whether PPARG activation (using the synthetic ligand pioglitazone (PGZ)) ameliorates the alterations in early ovarian function induced by androgen excess. Methods: Female prepubertal rats were treated with equine chorionic gonadotropin (eCG) to induce folliculogenesis, together with dehydroepiandrosterone (DHEA) to induce hyperandrogenism and/or PGZ to evaluate PPARG activation. We assessed i) very early ovarian folliculogenesis, ii) PPARG activation, iii) ovarian steroidogenic enzymes, iv) the estradiol/testosterone ratio, v) the ovarian inflammatory status and vi) oxidative stress. Results: PGZ prevented the inactivation of ovarian PPARG induced by androgen excess by increasing PPARG itself and the gene expression of PPARG-coactivator 1 alpha (PGC1A), and by decreasing the gene expression of nuclear co-repressor (NCOR). PGZ also prevented the altered ovarian steroidogenesis, pro-inflammatory status and oxidative stress induced by androgen excess. Conclusions: Our findings suggest that PPARG activation plays important roles in modulating early ovarian function, and highlight the importance of understanding the role(s) of PPARG activation in the ovary, and the possible involvement in the treatment of ovarian pathologies, and/or the impact in regulating/improving fertility.Fil: Velez, Leandro Martin. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Centro de Estudios Farmacológicos y Botánicos. Universidad de Buenos Aires. Facultad de Medicina. Centro de Estudios Farmacológicos y Botánicos; ArgentinaFil: Abruzzese, Giselle Adriana. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Centro de Estudios Farmacológicos y Botánicos. Universidad de Buenos Aires. Facultad de Medicina. Centro de Estudios Farmacológicos y Botánicos; ArgentinaFil: Heber, María Florencia. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Centro de Estudios Farmacológicos y Botánicos. Universidad de Buenos Aires. Facultad de Medicina. Centro de Estudios Farmacológicos y Botánicos; ArgentinaFil: Ferreira, Silvana Rocio. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Centro de Estudios Farmacológicos y Botánicos. Universidad de Buenos Aires. Facultad de Medicina. Centro de Estudios Farmacológicos y Botánicos; ArgentinaFil: Motta, Alicia Beatriz. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Centro de Estudios Farmacológicos y Botánicos. Universidad de Buenos Aires. Facultad de Medicina. Centro de Estudios Farmacológicos y Botánicos; ArgentinaPolish Acad Sciences Inst Pharmacology2019-02info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/120010Velez, Leandro Martin; Abruzzese, Giselle Adriana; Heber, María Florencia; Ferreira, Silvana Rocio; Motta, Alicia Beatriz; Treatment with the synthetic PPARG ligand pioglitazone ameliorates early ovarian alterations induced by dehydroepiandrosterone in prepubertal rats; Polish Acad Sciences Inst Pharmacology; Pharmacological Reports; 71; 1; 2-2019; 96-1041734-1140CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S1734114018301981?via%3Dihubinfo:eu-repo/semantics/altIdentifier/doi/10.1016/j.pharep.2018.09.009info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-nd/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2024-05-08T13:35:45Zoai:ri.conicet.gov.ar:11336/120010instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982024-05-08 13:35:45.665CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
score 15,81155

Similares en LA Referencia