MLST reveals a separate and novel clonal group for Acidovorax avenae strains causing red stripe in sugarcane from Argentina

Acidovorax species cause a wide range of economically important diseases in monocotyledonous and dicotyledonous plants, including sugarcane, corn, rice, oats, millet, foxtail watermelon and orchids. In Argentina, the red stripe disease of sugarcane caused by A. avenae affects 30% of the milling stem...

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Detalles Bibliográficos
Autores: Fontana, Paola Daniela, Tomasini, Nicolás, Fontana, Cecilia Alejandra, Di Pauli, Valentina, Cocconcelli, Pier Sandro, Vignolo, Graciela Margarita, Salazar, Sergio Miguel
Tipo de recurso: artículo
Estado:Versión aceptada para publicación
Fecha de publicación:2018
País:Argentina
Institución:Instituto Nacional de Tecnología Agropecuaria
Repositorio:INTA Digital (INTA)
Idioma:inglés
OAI Identifier:oai:localhost:20.500.12123/4207
Acceso en línea:http://hdl.handle.net/20.500.12123/4207
https://apsjournals.apsnet.org/doi/10.1094/PHYTO-08-18-0303-R
https://doi.org/10.1094/PHYTO-08-18-0303-R
Access Level:acceso abierto
Palabra clave:Caña de Azúcar
Enfermedades de las Plantas
Secuencia Nucleotídica
Genética
Genotipos
Sugarcane
Plant Diseases
Nucleotide Sequence
Genetics
Genotypes
Acidovorax avenae
Estría Roja de la Caña de Azúcar
MLST
Descripción
Sumario:Acidovorax species cause a wide range of economically important diseases in monocotyledonous and dicotyledonous plants, including sugarcane, corn, rice, oats, millet, foxtail watermelon and orchids. In Argentina, the red stripe disease of sugarcane caused by A. avenae affects 30% of the milling stems with important economic losses. To explore the genetic diversity of this bacterium associated with red stripe in Argentina, MLST was applied. This study included 15 local strains isolated from four different sugarcane planting regions and selected after RAPD analysis and reference strains of A. citrulli, A. avenae, and A. oryzae to investigate their phylogenetic relationships. MLST analysis resulted in five sequence types (STs) among the sugarcane A. avenae strains which constitute a clonal complex, meaning a common and close origin. Sugarcane strains were related to A. avenae from other hosts and distant to A. citrulli. Signals of frequent recombination in several lineages of A. avenae was detected and we observed that A. oryzae is closely related to A. avenae strains. This study provides valuable data in the field of epidemiological and evolutionary investigations of novel clone of A. avenae strains causing sugarcane red stripe. The knowledge of the genetic diversity and the specificity strain-host are important to select the genotypes with the best response to the red stripe disease.