Major cereal crops benefit from biological nitrogen fixation when inoculated with the nitrogen-fixing bacterium Pseudomonas protegens Pf-5 X940
A main goal of biological nitrogen fixation research has been to expand the nitrogen-fixing ability to major cereal crops. In this work, we demonstrate the use of the efficient nitrogen-fixing rhizobacterium Pseudomonas protegens Pf-5 X940 as a chassis to engineer the transfer of nitrogen fixed by B...
| Autores: | , , , , , , , , , , , |
|---|---|
| Tipo de recurso: | artículo |
| Estado: | Versión publicada |
| Fecha de publicación: | 2016 |
| País: | Argentina |
| Institución: | Instituto Nacional de Tecnología Agropecuaria |
| Repositorio: | INTA Digital (INTA) |
| Idioma: | inglés |
| OAI Identifier: | oai:localhost:20.500.12123/1105 |
| Acceso en línea: | http://hdl.handle.net/20.500.12123/1105 http://onlinelibrary.wiley.com/doi/10.1111/1462-2920.13376/epdf?r3_referer=wol&tracking_action=preview_click&show_checkout=1&purchase_referrer=onlinelibrary.wiley.com&purchase_site_license=LICENSE_DENIED |
| Access Level: | acceso abierto |
| Palabra clave: | Fijación Biológica del Nitrógeno Cultivos de Cereales Bacteria Fijadora del Nitrógeno Pseudomonas Biological Nitrogen Fixation Cereal Crops Nitrogen Fixing Bacteria |
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Major cereal crops benefit from biological nitrogen fixation when inoculated with the nitrogen-fixing bacterium Pseudomonas protegens Pf-5 X940 |
| title |
Major cereal crops benefit from biological nitrogen fixation when inoculated with the nitrogen-fixing bacterium Pseudomonas protegens Pf-5 X940 |
| spellingShingle |
Major cereal crops benefit from biological nitrogen fixation when inoculated with the nitrogen-fixing bacterium Pseudomonas protegens Pf-5 X940 Fox, Ana Romina Fijación Biológica del Nitrógeno Cultivos de Cereales Bacteria Fijadora del Nitrógeno Pseudomonas Biological Nitrogen Fixation Cereal Crops Nitrogen Fixing Bacteria |
| title_short |
Major cereal crops benefit from biological nitrogen fixation when inoculated with the nitrogen-fixing bacterium Pseudomonas protegens Pf-5 X940 |
| title_full |
Major cereal crops benefit from biological nitrogen fixation when inoculated with the nitrogen-fixing bacterium Pseudomonas protegens Pf-5 X940 |
| title_fullStr |
Major cereal crops benefit from biological nitrogen fixation when inoculated with the nitrogen-fixing bacterium Pseudomonas protegens Pf-5 X940 |
| title_full_unstemmed |
Major cereal crops benefit from biological nitrogen fixation when inoculated with the nitrogen-fixing bacterium Pseudomonas protegens Pf-5 X940 |
| title_sort |
Major cereal crops benefit from biological nitrogen fixation when inoculated with the nitrogen-fixing bacterium Pseudomonas protegens Pf-5 X940 |
| dc.creator.none.fl_str_mv |
Fox, Ana Romina Soto, Gabriela Cinthia Valverde, Claudio Fabián Russo, Daniela Marta Lagares, Antonio Zorreguieta, Ángeles Pascuan, Cecilia Gabriela Frare, Romina Alejandra Mercado Blanco, Jesús Dixon, Ray Ayub, Nicolás Daniel Alleva, Karina Edith |
| author |
Fox, Ana Romina |
| author_facet |
Fox, Ana Romina Soto, Gabriela Cinthia Valverde, Claudio Fabián Russo, Daniela Marta Lagares, Antonio Zorreguieta, Ángeles Pascuan, Cecilia Gabriela Frare, Romina Alejandra Mercado Blanco, Jesús Dixon, Ray Ayub, Nicolás Daniel Alleva, Karina Edith |
| author_role |
author |
| author2 |
Soto, Gabriela Cinthia Valverde, Claudio Fabián Russo, Daniela Marta Lagares, Antonio Zorreguieta, Ángeles Pascuan, Cecilia Gabriela Frare, Romina Alejandra Mercado Blanco, Jesús Dixon, Ray Ayub, Nicolás Daniel Alleva, Karina Edith |
| author2_role |
author author author author author author author author author author author |
| dc.subject.none.fl_str_mv |
Fijación Biológica del Nitrógeno Cultivos de Cereales Bacteria Fijadora del Nitrógeno Pseudomonas Biological Nitrogen Fixation Cereal Crops Nitrogen Fixing Bacteria |
| topic |
Fijación Biológica del Nitrógeno Cultivos de Cereales Bacteria Fijadora del Nitrógeno Pseudomonas Biological Nitrogen Fixation Cereal Crops Nitrogen Fixing Bacteria |
| description |
A main goal of biological nitrogen fixation research has been to expand the nitrogen-fixing ability to major cereal crops. In this work, we demonstrate the use of the efficient nitrogen-fixing rhizobacterium Pseudomonas protegens Pf-5 X940 as a chassis to engineer the transfer of nitrogen fixed by BNF to maize and wheat under non-gnotobiotic conditions. Inoculation of maize and wheat with Pf-5 X940 largely improved nitrogen content and biomass accumulation in both vegetative and reproductive tissues, and this beneficial effect was positively associated with high nitrogen fixation rates in roots. 15N isotope dilution analysis showed that maize and wheat plants obtained substantial amounts of fixed nitrogen from the atmosphere. Pf-5 X940-GFP-tagged cells were always reisolated from the maize and wheat root surface but never from the inner root tissues. Confocal laser scanning microscopy confirmed root surface colonization of Pf-5 X940-GFP in wheat plants, and microcolonies were mostly visualized at the junctions between epidermal root cells. Genetic analysis using biofilm formation-related Pseudomonas mutants confirmed the relevance of bacterial root adhesion in the increase in nitrogen content, biomass accumulation and nitrogen fixation rates in wheat roots. To our knowledge, this is the first report of robust BNF in major cereal crops. |
| publishDate |
2016 |
| dc.date.none.fl_str_mv |
2016 2017-09-01T14:40:07Z 2017-09-01T14:40:07Z |
| dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
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article |
| status_str |
publishedVersion |
| dc.identifier.none.fl_str_mv |
http://hdl.handle.net/20.500.12123/1105 http://onlinelibrary.wiley.com/doi/10.1111/1462-2920.13376/epdf?r3_referer=wol&tracking_action=preview_click&show_checkout=1&purchase_referrer=onlinelibrary.wiley.com&purchase_site_license=LICENSE_DENIED 1462-2920 (Online) DOI: 10.1111/1462-2920.13376 |
| url |
http://hdl.handle.net/20.500.12123/1105 http://onlinelibrary.wiley.com/doi/10.1111/1462-2920.13376/epdf?r3_referer=wol&tracking_action=preview_click&show_checkout=1&purchase_referrer=onlinelibrary.wiley.com&purchase_site_license=LICENSE_DENIED |
| identifier_str_mv |
1462-2920 (Online) DOI: 10.1111/1462-2920.13376 |
| dc.language.none.fl_str_mv |
eng |
| language |
eng |
| dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) |
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openAccess |
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http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) |
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application/pdf |
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Environmental microbiology 18 (10) : 3522–3534. (October 2016) reponame:INTA Digital (INTA) instname:Instituto Nacional de Tecnología Agropecuaria |
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Instituto Nacional de Tecnología Agropecuaria |
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INTA Digital (INTA) |
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INTA Digital (INTA) |
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INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria |
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tripaldi.nicolas@inta.gob.ar |
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1799197018219872256 |
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Major cereal crops benefit from biological nitrogen fixation when inoculated with the nitrogen-fixing bacterium Pseudomonas protegens Pf-5 X940Fox, Ana RominaSoto, Gabriela CinthiaValverde, Claudio FabiánRusso, Daniela MartaLagares, AntonioZorreguieta, ÁngelesPascuan, Cecilia GabrielaFrare, Romina AlejandraMercado Blanco, JesúsDixon, RayAyub, Nicolás DanielAlleva, Karina EdithFijación Biológica del NitrógenoCultivos de CerealesBacteria Fijadora del NitrógenoPseudomonasBiological Nitrogen FixationCereal CropsNitrogen Fixing BacteriaA main goal of biological nitrogen fixation research has been to expand the nitrogen-fixing ability to major cereal crops. In this work, we demonstrate the use of the efficient nitrogen-fixing rhizobacterium Pseudomonas protegens Pf-5 X940 as a chassis to engineer the transfer of nitrogen fixed by BNF to maize and wheat under non-gnotobiotic conditions. Inoculation of maize and wheat with Pf-5 X940 largely improved nitrogen content and biomass accumulation in both vegetative and reproductive tissues, and this beneficial effect was positively associated with high nitrogen fixation rates in roots. 15N isotope dilution analysis showed that maize and wheat plants obtained substantial amounts of fixed nitrogen from the atmosphere. Pf-5 X940-GFP-tagged cells were always reisolated from the maize and wheat root surface but never from the inner root tissues. Confocal laser scanning microscopy confirmed root surface colonization of Pf-5 X940-GFP in wheat plants, and microcolonies were mostly visualized at the junctions between epidermal root cells. Genetic analysis using biofilm formation-related Pseudomonas mutants confirmed the relevance of bacterial root adhesion in the increase in nitrogen content, biomass accumulation and nitrogen fixation rates in wheat roots. To our knowledge, this is the first report of robust BNF in major cereal crops.Inst. de Genética "Ewald A. Favret"- IGEAFFil: Fox, Ana Romina. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética "Ewald A. Favret"; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Soto, Gabriela Cinthia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética "Ewald A. Favret"; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Valverde, Claudio Fabián. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología; ArgentinaFil: Russo, Daniela Marta. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquímicas de Buenos Aires. Fundación Instituto Leloir. Instituto de Investigaciones Bioquímicas de Buenos Aires; ArgentinaFil: Lagares, Antonio. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología; ArgentinaFil: Zorreguieta, Angeles. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquímicas de Buenos Aires. Fundación Instituto Leloir. Instituto de Investigaciones Bioquímicas de Buenos Aires; ArgentinaFil: Alleva, Karina Edith. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Biodiversidad y Biología Experimental; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Pascuan, Cecilia Gabriela. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética "Ewald A. Favret"; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Frare, Romina Alejandra. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética "Ewald A. Favret"; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Mercado Blanco, Jesús. Consejo Superior de Investigaciones Científicas. Instituto de Agricultura Sostenible. Departamento de Protección de Cultivos; EspañaFil: Dixon, Ray. John Innes Centre. Department of Molecular Microbiology; Reino UnidoFil: Ayub, Nicolás Daniel. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética "Ewald A. Favret"; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina2017-09-01T14:40:07Z2017-09-01T14:40:07Z2016info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/1105http://onlinelibrary.wiley.com/doi/10.1111/1462-2920.13376/epdf?r3_referer=wol&tracking_action=preview_click&show_checkout=1&purchase_referrer=onlinelibrary.wiley.com&purchase_site_license=LICENSE_DENIED1462-2920 (Online)DOI: 10.1111/1462-2920.13376Environmental microbiology 18 (10) : 3522–3534. (October 2016)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)2024-05-15T12:23:28Zoai:localhost:20.500.12123/1105instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2024-05-15 12:23:28.331INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse |
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15,812429 |