Cross-species transfer of SSR markers in Setaria sphacelata and Trichloris crinita sp. = Transferencia cruzada de marcadores SSR en Setaria sphacelata y Trichloris crinita sp.

Setaria sphacelata and Trichloris crinita are subtropical forage species that are important for livestock breeding in Argentina. Genomic information is scarce for these species, and there are no molecular markers designed for them; this limits the development of genetic improvement programs. We perf...

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Detalles Bibliográficos
Autores: Randazzo, Cecilia, Ferri, Andrea Matilde, Carabajal Paladino, Leonela Zusel, Andres, Adriana Noemi, Ingala, Lorena Romina
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2019
País:Argentina
Institución:Instituto Nacional de Tecnología Agropecuaria
Repositorio:INTA Digital (INTA)
Idioma:inglés
OAI Identifier:oai:localhost:20.500.12123/8708
Acceso en línea:https://revistas.unal.edu.co/index.php/agrocol/article/view/78785
http://hdl.handle.net/20.500.12123/8708
https://doi.org/10.15446/agron.colomb.v37n2.78785
Access Level:acceso abierto
Palabra clave:Polymorphism
Genetic Variation
Plant Genetic Resources
Feed Crops
Genetic Markers
Polimorfismo
Variación Genética
Recursos Genéticos Vegetales
Plantas Forrajeras
Setaria sphacelata
Marcadores Genéticos
Trichloris crinita
Descripción
Sumario:Setaria sphacelata and Trichloris crinita are subtropical forage species that are important for livestock breeding in Argentina. Genomic information is scarce for these species, and there are no molecular markers designed for them; this limits the development of genetic improvement programs. We performed a cross-species transfer of SSR markers from several Poaceae species. In S. sphacelata, 8 SSR markers were transferred from Setaria italica (40% transfer rate), exhibiting 83% polymorphism. Kazungula, Splenda and Narok cultivars were genetically differentiated and the experimental material “Selección INTA” was separated from Narok, from which it was derived. For T. crinita, 19 microsatellites were transferred from 5 Poaceae species (7.3% transfer rate), with 69% polymorphism. The results obtained in this study show the potential of the transferred SSR markers for assessing genetic variation and for expanding the genetic resources available for these species.