Development of a nanostabilized biocatalyst using an extremophilic microorganism for ribavirin biosynthesis
Ribavirin is a guanosine analogue commonly used as an antiviral compound for the treatment of Hepatitis C virus (HCV) infection. The biosynthesis of this compound using Geobacillus kaustophilus ATCC 8005 as biocatalyst is herein reported. This extremophilic microorganism has been successfully entrap...
| Autores: | , , |
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| Tipo de recurso: | artículo |
| Estado: | Versión publicada |
| Fecha de publicación: | 2015 |
| País: | Argentina |
| Institución: | Consejo Nacional de Investigaciones Científicas y Técnicas |
| Repositorio: | CONICET Digital (CONICET) |
| Idioma: | inglés |
| OAI Identifier: | oai:ri.conicet.gov.ar:11336/41727 |
| Acceso en línea: | http://hdl.handle.net/11336/41727 |
| Access Level: | acceso abierto |
| Palabra clave: | Thermophilic Biocatalyst Anti Hcv Compound Bentonite Scale-Up https://purl.org/becyt/ford/2.9 https://purl.org/becyt/ford/2 |
| Sumario: | Ribavirin is a guanosine analogue commonly used as an antiviral compound for the treatment of Hepatitis C virus (HCV) infection. The biosynthesis of this compound using Geobacillus kaustophilus ATCC 8005 as biocatalyst is herein reported. This extremophilic microorganism has been successfully entrapped in an agarose matrix supplemented with bentonite, which was defined as bionanocomposite. This immobilized biocatalyst was stable for more than 580 h without activity loss, significantly improving operational stability and mechanical properties over the conventional agarose matrix. Furthermore, a packed-bed bioreactor for bioprocess scale-up was designed, which was able to produce 370 mg L−1 of ribavirin. In conclusion, a smooth, inexpensive and environmentally friendly method to obtain ribavirin was developed in this study. |
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