New method for electroporation of Lactobacillus species grown in high salt

We here describe a new method for electroporation of Lactobacillus species, obligately homofermentative and facultatively heterofermentative, based on the cell-wall weakening resulting from growth in high-salt media. For L. casei, optimum transformation efficiency of up to 105 transformants per micr...

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Detalles Bibliográficos
Autores: Palomino, Maria Mercedes, Allievi, Mariana Caludia, Prado Acosta, Mariano, Sanchez Rivas, Carmen, Ruzal, Sandra Mónica
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2010
País:Argentina
Institución:Consejo Nacional de Investigaciones Científicas y Técnicas
Repositorio:CONICET Digital (CONICET)
Idioma:inglés
OAI Identifier:oai:ri.conicet.gov.ar:11336/93755
Acceso en línea:http://hdl.handle.net/11336/93755
Access Level:acceso abierto
Palabra clave:ELECTROPORATION
HIGH SALT GROWTH
LACTOBACILLUS
https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
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spelling New method for electroporation of Lactobacillus species grown in high saltPalomino, Maria MercedesAllievi, Mariana CaludiaPrado Acosta, MarianoSanchez Rivas, CarmenRuzal, Sandra MónicaELECTROPORATIONHIGH SALT GROWTHLACTOBACILLUShttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1https://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1We here describe a new method for electroporation of Lactobacillus species, obligately homofermentative and facultatively heterofermentative, based on the cell-wall weakening resulting from growth in high-salt media. For L. casei, optimum transformation efficiency of up to 105 transformants per microgram of plasmid DNA was achieved following growth in the presence of 0.9M NaCl. Plasmids of different sizes and replication origins were also similarly transformed. These competent cells could be used either directly or stored frozen, up to 1month, for future use, with similar efficiency. This protocol was assayed with different Lactobacillus species: L. delbrueckii subsp. lactis, L. paracasei, L. plantarum and L. acidophilus, and it was found that they were transformed with similar efficiency.Fil: Palomino, Maria Mercedes. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Biológica; ArgentinaFil: Allievi, Mariana Caludia. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Biológica; ArgentinaFil: Prado Acosta, Mariano. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Biológica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Sanchez Rivas, Carmen. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Biológica; ArgentinaFil: Ruzal, Sandra Mónica. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Biológica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaElsevier Science2010-11info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/93755Palomino, Maria Mercedes; Allievi, Mariana Caludia; Prado Acosta, Mariano; Sanchez Rivas, Carmen; Ruzal, Sandra Mónica; New method for electroporation of Lactobacillus species grown in high salt; Elsevier Science; Journal of Microbiological Methods; 83; 2; 11-2010; 164-1670167-7012CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0167701210002836info:eu-repo/semantics/altIdentifier/doi/10.1016/j.mimet.2010.08.017info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2024-05-08T13:44:53Zoai:ri.conicet.gov.ar:11336/93755instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982024-05-08 13:44:53.765CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv New method for electroporation of Lactobacillus species grown in high salt
title New method for electroporation of Lactobacillus species grown in high salt
spellingShingle New method for electroporation of Lactobacillus species grown in high salt
Palomino, Maria Mercedes
ELECTROPORATION
HIGH SALT GROWTH
LACTOBACILLUS
https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
title_short New method for electroporation of Lactobacillus species grown in high salt
title_full New method for electroporation of Lactobacillus species grown in high salt
title_fullStr New method for electroporation of Lactobacillus species grown in high salt
title_full_unstemmed New method for electroporation of Lactobacillus species grown in high salt
title_sort New method for electroporation of Lactobacillus species grown in high salt
dc.creator.none.fl_str_mv Palomino, Maria Mercedes
Allievi, Mariana Caludia
Prado Acosta, Mariano
Sanchez Rivas, Carmen
Ruzal, Sandra Mónica
author Palomino, Maria Mercedes
author_facet Palomino, Maria Mercedes
Allievi, Mariana Caludia
Prado Acosta, Mariano
Sanchez Rivas, Carmen
Ruzal, Sandra Mónica
author_role author
author2 Allievi, Mariana Caludia
Prado Acosta, Mariano
Sanchez Rivas, Carmen
Ruzal, Sandra Mónica
author2_role author
author
author
author
dc.subject.none.fl_str_mv ELECTROPORATION
HIGH SALT GROWTH
LACTOBACILLUS
https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
topic ELECTROPORATION
HIGH SALT GROWTH
LACTOBACILLUS
https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
description We here describe a new method for electroporation of Lactobacillus species, obligately homofermentative and facultatively heterofermentative, based on the cell-wall weakening resulting from growth in high-salt media. For L. casei, optimum transformation efficiency of up to 105 transformants per microgram of plasmid DNA was achieved following growth in the presence of 0.9M NaCl. Plasmids of different sizes and replication origins were also similarly transformed. These competent cells could be used either directly or stored frozen, up to 1month, for future use, with similar efficiency. This protocol was assayed with different Lactobacillus species: L. delbrueckii subsp. lactis, L. paracasei, L. plantarum and L. acidophilus, and it was found that they were transformed with similar efficiency.
publishDate 2010
dc.date.none.fl_str_mv 2010-11
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/93755
Palomino, Maria Mercedes; Allievi, Mariana Caludia; Prado Acosta, Mariano; Sanchez Rivas, Carmen; Ruzal, Sandra Mónica; New method for electroporation of Lactobacillus species grown in high salt; Elsevier Science; Journal of Microbiological Methods; 83; 2; 11-2010; 164-167
0167-7012
CONICET Digital
CONICET
url http://hdl.handle.net/11336/93755
identifier_str_mv Palomino, Maria Mercedes; Allievi, Mariana Caludia; Prado Acosta, Mariano; Sanchez Rivas, Carmen; Ruzal, Sandra Mónica; New method for electroporation of Lactobacillus species grown in high salt; Elsevier Science; Journal of Microbiological Methods; 83; 2; 11-2010; 164-167
0167-7012
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0167701210002836
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.mimet.2010.08.017
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier Science
publisher.none.fl_str_mv Elsevier Science
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 15.812429

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