Age-related changes in the metabolization of phosphatidic acid in cerebral cortex synaptosomes

In this study, phosphatidic acid (PA) metabolization is found to generate diacylglycerol (DAG), monoacylglycerol (MAG) and glycerol by the sequential action of lipid phosphate phosphatase (LPP), diacylglycerol lipase (DAGL), and monoacylglycerol lipase (MAGL) in cerebral cortex (CC) synaptosomes. It...

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Detalles Bibliográficos
Autores: Pasquaré, Susana Juana, Gaveglio, Virginia Lucía, Giusto, Norma Maria
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2009
País:Argentina
Institución:Consejo Nacional de Investigaciones Científicas y Técnicas
Repositorio:CONICET Digital (CONICET)
Idioma:inglés
OAI Identifier:oai:ri.conicet.gov.ar:11336/41898
Acceso en línea:http://hdl.handle.net/11336/41898
Access Level:acceso abierto
Palabra clave:Aging
Cerebral Cortex
Phosphatidic Acid
Phospholipid Metabolism
https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
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spelling Age-related changes in the metabolization of phosphatidic acid in cerebral cortex synaptosomesPasquaré, Susana JuanaGaveglio, Virginia LucíaGiusto, Norma MariaAgingCerebral CortexPhosphatidic AcidPhospholipid Metabolismhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1In this study, phosphatidic acid (PA) metabolization is found to generate diacylglycerol (DAG), monoacylglycerol (MAG) and glycerol by the sequential action of lipid phosphate phosphatase (LPP), diacylglycerol lipase (DAGL), and monoacylglycerol lipase (MAGL) in cerebral cortex (CC) synaptosomes. It is also demonstrated that PA is metabolized by phospholipases A (PLA)/lysophosphatidic acid phosphohydrolase (LPAPase) in synaptic endings. Age-related changes in the metabolization of PA have been observed in rat cerebral cortex synaptosomes in the presence of the alternative substrates for LPP, namely LPA, sphingosine 1-phosphate (S1P) and ceramide 1-phosphate (C1P). In addition, LPA and C1P up to concentrations of about 50 μM favor the metabolism in the direction of MAG and glycerol in aged and adult synaptosomes, respectively. At equimolecular concentrations with PA, LPA decreases DAG formation in adult and aged synaptosomes, whereas S1P decreases it and C1P increases it only in aged synaptosomes. Sphingosine (50 μM) or ceramide (100 μM) increase PA metabolism by the pathway that involves LPP/DAGL/MAGL action in aged membranes. Using RHC-80267, a DAGL inhibitor, we could observe that 50% and 33% of MAG are produced as a result of DAGL action in adult and aged synaptosomes, respectively. Taken together, our findings indicate that the ageing modifies the different enzymatic pathways involved in PA metabolization.Fil: Pasquaré, Susana Juana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; ArgentinaFil: Gaveglio, Virginia Lucía. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; ArgentinaFil: Giusto, Norma Maria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; ArgentinaElsevier Science Inc2009-08-15info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/41898Pasquaré, Susana Juana; Gaveglio, Virginia Lucía; Giusto, Norma Maria; Age-related changes in the metabolization of phosphatidic acid in cerebral cortex synaptosomes; Elsevier Science Inc; Archives of Biochemistry and Biophysics; 488; 2; 15-8-2009; 121-1290003-9861CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1016/j.abb.2009.07.002info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0003986109002173info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2024-05-08T14:23:39Zoai:ri.conicet.gov.ar:11336/41898instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982024-05-08 14:23:39.772CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Age-related changes in the metabolization of phosphatidic acid in cerebral cortex synaptosomes
title Age-related changes in the metabolization of phosphatidic acid in cerebral cortex synaptosomes
spellingShingle Age-related changes in the metabolization of phosphatidic acid in cerebral cortex synaptosomes
Pasquaré, Susana Juana
Aging
Cerebral Cortex
Phosphatidic Acid
Phospholipid Metabolism
https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
title_short Age-related changes in the metabolization of phosphatidic acid in cerebral cortex synaptosomes
title_full Age-related changes in the metabolization of phosphatidic acid in cerebral cortex synaptosomes
title_fullStr Age-related changes in the metabolization of phosphatidic acid in cerebral cortex synaptosomes
title_full_unstemmed Age-related changes in the metabolization of phosphatidic acid in cerebral cortex synaptosomes
title_sort Age-related changes in the metabolization of phosphatidic acid in cerebral cortex synaptosomes
dc.creator.none.fl_str_mv Pasquaré, Susana Juana
Gaveglio, Virginia Lucía
Giusto, Norma Maria
author Pasquaré, Susana Juana
author_facet Pasquaré, Susana Juana
Gaveglio, Virginia Lucía
Giusto, Norma Maria
author_role author
author2 Gaveglio, Virginia Lucía
Giusto, Norma Maria
author2_role author
author
dc.subject.none.fl_str_mv Aging
Cerebral Cortex
Phosphatidic Acid
Phospholipid Metabolism
https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
topic Aging
Cerebral Cortex
Phosphatidic Acid
Phospholipid Metabolism
https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
description In this study, phosphatidic acid (PA) metabolization is found to generate diacylglycerol (DAG), monoacylglycerol (MAG) and glycerol by the sequential action of lipid phosphate phosphatase (LPP), diacylglycerol lipase (DAGL), and monoacylglycerol lipase (MAGL) in cerebral cortex (CC) synaptosomes. It is also demonstrated that PA is metabolized by phospholipases A (PLA)/lysophosphatidic acid phosphohydrolase (LPAPase) in synaptic endings. Age-related changes in the metabolization of PA have been observed in rat cerebral cortex synaptosomes in the presence of the alternative substrates for LPP, namely LPA, sphingosine 1-phosphate (S1P) and ceramide 1-phosphate (C1P). In addition, LPA and C1P up to concentrations of about 50 μM favor the metabolism in the direction of MAG and glycerol in aged and adult synaptosomes, respectively. At equimolecular concentrations with PA, LPA decreases DAG formation in adult and aged synaptosomes, whereas S1P decreases it and C1P increases it only in aged synaptosomes. Sphingosine (50 μM) or ceramide (100 μM) increase PA metabolism by the pathway that involves LPP/DAGL/MAGL action in aged membranes. Using RHC-80267, a DAGL inhibitor, we could observe that 50% and 33% of MAG are produced as a result of DAGL action in adult and aged synaptosomes, respectively. Taken together, our findings indicate that the ageing modifies the different enzymatic pathways involved in PA metabolization.
publishDate 2009
dc.date.none.fl_str_mv 2009-08-15
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/41898
Pasquaré, Susana Juana; Gaveglio, Virginia Lucía; Giusto, Norma Maria; Age-related changes in the metabolization of phosphatidic acid in cerebral cortex synaptosomes; Elsevier Science Inc; Archives of Biochemistry and Biophysics; 488; 2; 15-8-2009; 121-129
0003-9861
CONICET Digital
CONICET
url http://hdl.handle.net/11336/41898
identifier_str_mv Pasquaré, Susana Juana; Gaveglio, Virginia Lucía; Giusto, Norma Maria; Age-related changes in the metabolization of phosphatidic acid in cerebral cortex synaptosomes; Elsevier Science Inc; Archives of Biochemistry and Biophysics; 488; 2; 15-8-2009; 121-129
0003-9861
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1016/j.abb.2009.07.002
info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0003986109002173
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier Science Inc
publisher.none.fl_str_mv Elsevier Science Inc
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 15.811543

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