Scopolamine and hyoscyamine production by hairy root cultures of Brugmansia candida: Influence of calcium chloride, hemicellulase and theophylline

CaCl2 (50 mM) and hemicellulase (0.5 U mg-1) increased the intracellular accumulation (60-250%), release (60-200%) and production (45-200%) of hyoscyamine and scopolamine in hairy roots of Brugmansia candida. Theophylline (0.25 mM), alone or in combination with hemicellulase, was ineffective in incr...

Descripción completa

Detalles Bibliográficos
Autores: Pitta Alvarez, Sandra Irene, Spollansky, T. C., Giulietti, Ana Maria
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2000
País:Argentina
Institución:Consejo Nacional de Investigaciones Científicas y Técnicas
Repositorio:CONICET Digital (CONICET)
Idioma:inglés
OAI Identifier:oai:ri.conicet.gov.ar:11336/39227
Acceso en línea:http://hdl.handle.net/11336/39227
Access Level:acceso abierto
Palabra clave:Brugmansia Candida
Hairy Root Cultures
Hyoscyamine
Scopolamine
https://purl.org/becyt/ford/2.9
https://purl.org/becyt/ford/2
Descripción
Sumario:CaCl2 (50 mM) and hemicellulase (0.5 U mg-1) increased the intracellular accumulation (60-250%), release (60-200%) and production (45-200%) of hyoscyamine and scopolamine in hairy roots of Brugmansia candida. Theophylline (0.25 mM), alone or in combination with hemicellulase, was ineffective in increasing hyoscyamine and scopolamine production.