Determination of the efective radiation dose for mutation breeding in purple carrot (Daucus carota L.) and possible variations formed

Background Plant breeding allows altering the genetic structure of plants to meet human needs. The use of radiation technology for inducing mutations and -thereby- new phenotypic variants has become increasingly common as a tool for developing new crops. The aim of this study was to determine the ef...

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Detalhes bibliográficos
Autores: Gülistan Yarar, Metin Kocak, Nihal Denli, Cavagnaro, Pablo Federico, Mehtap Yildiz
Formato: artículo
Estado:Versión publicada
Fecha de publicación:2021
País:Argentina
Recursos:Consejo Nacional de Investigaciones Científicas y Técnicas
Repositorio:CONICET Digital (CONICET)
Idioma:inglés
OAI Identifier:oai:ri.conicet.gov.ar:11336/154510
Acesso em linha:http://hdl.handle.net/11336/154510
Access Level:acceso abierto
Palavra-chave:EFFICIENT MUTAGEN DOSE
PURPLE CARROT
MUTATION
ISSR
SRAP
https://purl.org/becyt/ford/4.1
https://purl.org/becyt/ford/4
id AR_0bcc1efa402e9e83ee88aa3e89fe322c
oai_identifier_str oai:ri.conicet.gov.ar:11336/154510
network_acronym_str AR
network_name_str Argentina
repository_id_str
dc.title.none.fl_str_mv Determination of the efective radiation dose for mutation breeding in purple carrot (Daucus carota L.) and possible variations formed
title Determination of the efective radiation dose for mutation breeding in purple carrot (Daucus carota L.) and possible variations formed
spellingShingle Determination of the efective radiation dose for mutation breeding in purple carrot (Daucus carota L.) and possible variations formed
Gülistan Yarar
EFFICIENT MUTAGEN DOSE
PURPLE CARROT
MUTATION
ISSR
SRAP
https://purl.org/becyt/ford/4.1
https://purl.org/becyt/ford/4
title_short Determination of the efective radiation dose for mutation breeding in purple carrot (Daucus carota L.) and possible variations formed
title_full Determination of the efective radiation dose for mutation breeding in purple carrot (Daucus carota L.) and possible variations formed
title_fullStr Determination of the efective radiation dose for mutation breeding in purple carrot (Daucus carota L.) and possible variations formed
title_full_unstemmed Determination of the efective radiation dose for mutation breeding in purple carrot (Daucus carota L.) and possible variations formed
title_sort Determination of the efective radiation dose for mutation breeding in purple carrot (Daucus carota L.) and possible variations formed
dc.creator.none.fl_str_mv Gülistan Yarar
Metin Kocak
Nihal Denli
Cavagnaro, Pablo Federico
Mehtap Yildiz
author Gülistan Yarar
author_facet Gülistan Yarar
Metin Kocak
Nihal Denli
Cavagnaro, Pablo Federico
Mehtap Yildiz
author_role author
author2 Metin Kocak
Nihal Denli
Cavagnaro, Pablo Federico
Mehtap Yildiz
author2_role author
author
author
author
dc.subject.none.fl_str_mv EFFICIENT MUTAGEN DOSE
PURPLE CARROT
MUTATION
ISSR
SRAP
https://purl.org/becyt/ford/4.1
https://purl.org/becyt/ford/4
topic EFFICIENT MUTAGEN DOSE
PURPLE CARROT
MUTATION
ISSR
SRAP
https://purl.org/becyt/ford/4.1
https://purl.org/becyt/ford/4
description Background Plant breeding allows altering the genetic structure of plants to meet human needs. The use of radiation technology for inducing mutations and -thereby- new phenotypic variants has become increasingly common as a tool for developing new crops. The aim of this study was to determine the efective gamma irradiation dose for inducing mutations in purple carrot. Methods and results Increasing gamma radiation doses [0, 50, 100, 200, 300, 400, 500, and 600 Gy] were applied to purple carrot seeds. The irradiated seeds were sown in pots and the emergence and survival rates of the seedlings were analyzed. Considering plant emergence (%) as a response variable, the LD50 dose was 387.5 Gy. Analysis of root length, root width (shoulder diameter) and plant height in control (0 Gy) and irradiated plants (50–600 Gy) revealed an inverse association between these morphological traits and radiation dose. SRAP and ISSR markers were used to identify DNA polymorphisms in irradiated and control plants. The range of amplicons per primer set revealed by ISSR and SRAP markers was 4–10 and 2–13, respectively. In the ISSR analysis of the irradiated carrots (for the 8 doses used), we obtained range values for the average Nei’s gene diversity, Shannon’s information index, and polymorphism information content (PIC) of 0.13–0.25, 0.20–0.35, and 1.39–1.67, respectively, whereas in the SRAP analysis, the range values for these parameters were 0.15–0.25, 0.23–0.37, and 0.43–0.58, respectively. Cluster analysis revealed three main groups; (a) non-irradiated (control) plants, (b) plants from the 600 Gy dose, and (c) a third group with two subgroups: one with individuals from the lowest irradiation doses (50–200 Gy) and a second group with individuals from the highest irradiation doses (300–500 Gy). Conclusions This is the frst report on determining efective mutagen doses and genetic characterization of induced mutagenesis via gamma irradiation in purple carrot. ISSR and SRAP markers were successful in detecting variations among diferent levels of mutagen doses.
publishDate 2021
dc.date.none.fl_str_mv 2021-08-07
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/154510
Gülistan Yarar; Metin Kocak; Nihal Denli; Cavagnaro, Pablo Federico; Mehtap Yildiz; Determination of the efective radiation dose for mutation breeding in purple carrot (Daucus carota L.) and possible variations formed; Springer; Molecular Biology Reports; 7-8-2021; 1-10
0301-4851
CONICET Digital
CONICET
url http://hdl.handle.net/11336/154510
identifier_str_mv Gülistan Yarar; Metin Kocak; Nihal Denli; Cavagnaro, Pablo Federico; Mehtap Yildiz; Determination of the efective radiation dose for mutation breeding in purple carrot (Daucus carota L.) and possible variations formed; Springer; Molecular Biology Reports; 7-8-2021; 1-10
0301-4851
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://link.springer.com/article/10.1007/s11033-021-06618-0
info:eu-repo/semantics/altIdentifier/doi/10.1007/s11033-021-06618-0
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Springer
publisher.none.fl_str_mv Springer
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1799195477681373184
spelling Determination of the efective radiation dose for mutation breeding in purple carrot (Daucus carota L.) and possible variations formedGülistan YararMetin KocakNihal DenliCavagnaro, Pablo FedericoMehtap YildizEFFICIENT MUTAGEN DOSEPURPLE CARROTMUTATIONISSRSRAPhttps://purl.org/becyt/ford/4.1https://purl.org/becyt/ford/4Background Plant breeding allows altering the genetic structure of plants to meet human needs. The use of radiation technology for inducing mutations and -thereby- new phenotypic variants has become increasingly common as a tool for developing new crops. The aim of this study was to determine the efective gamma irradiation dose for inducing mutations in purple carrot. Methods and results Increasing gamma radiation doses [0, 50, 100, 200, 300, 400, 500, and 600 Gy] were applied to purple carrot seeds. The irradiated seeds were sown in pots and the emergence and survival rates of the seedlings were analyzed. Considering plant emergence (%) as a response variable, the LD50 dose was 387.5 Gy. Analysis of root length, root width (shoulder diameter) and plant height in control (0 Gy) and irradiated plants (50–600 Gy) revealed an inverse association between these morphological traits and radiation dose. SRAP and ISSR markers were used to identify DNA polymorphisms in irradiated and control plants. The range of amplicons per primer set revealed by ISSR and SRAP markers was 4–10 and 2–13, respectively. In the ISSR analysis of the irradiated carrots (for the 8 doses used), we obtained range values for the average Nei’s gene diversity, Shannon’s information index, and polymorphism information content (PIC) of 0.13–0.25, 0.20–0.35, and 1.39–1.67, respectively, whereas in the SRAP analysis, the range values for these parameters were 0.15–0.25, 0.23–0.37, and 0.43–0.58, respectively. Cluster analysis revealed three main groups; (a) non-irradiated (control) plants, (b) plants from the 600 Gy dose, and (c) a third group with two subgroups: one with individuals from the lowest irradiation doses (50–200 Gy) and a second group with individuals from the highest irradiation doses (300–500 Gy). Conclusions This is the frst report on determining efective mutagen doses and genetic characterization of induced mutagenesis via gamma irradiation in purple carrot. ISSR and SRAP markers were successful in detecting variations among diferent levels of mutagen doses.Fil: Gülistan Yarar. Van Yuzuncu Yil University; TurquíaFil: Metin Kocak. Van Yuzuncu Yil University; TurquíaFil: Nihal Denli. Alata Horticultural Research Institute; TurquíaFil: Cavagnaro, Pablo Federico. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Mendoza-San Juan. Estación Experimental Agropecuaria La Consulta; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza; ArgentinaFil: Mehtap Yildiz. Alata Horticultural Research Institute, Mersin, Turkey; TurquíaSpringer2021-08-07info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/154510Gülistan Yarar; Metin Kocak; Nihal Denli; Cavagnaro, Pablo Federico; Mehtap Yildiz; Determination of the efective radiation dose for mutation breeding in purple carrot (Daucus carota L.) and possible variations formed; Springer; Molecular Biology Reports; 7-8-2021; 1-100301-4851CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://link.springer.com/article/10.1007/s11033-021-06618-0info:eu-repo/semantics/altIdentifier/doi/10.1007/s11033-021-06618-0info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2024-05-08T13:53:42Zoai:ri.conicet.gov.ar:11336/154510instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982024-05-08 13:53:43.098CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
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