Room temperature fluorescence spectroscopy of benzo[a]pyrene metabolites on octadecyl extraction membranes
Benzo[a]pyrene (B[a]P) is a prototypic carcinogenic polycyclic aromatic hydrocarbon (PAH), which requires metabolic activation to produce its detrimental effects. Measurement of B[a]P metabolites in human urine could provide a direct way to assess individual differences in susceptibility to PAH-rela...
| Autores: | , , , , , |
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| Tipo de recurso: | artículo |
| Estado: | Versión publicada |
| Fecha de publicación: | 2016 |
| País: | Argentina |
| Institución: | Consejo Nacional de Investigaciones Científicas y Técnicas |
| Repositorio: | CONICET Digital (CONICET) |
| Idioma: | inglés |
| OAI Identifier: | oai:ri.conicet.gov.ar:11336/23828 |
| Acceso en línea: | http://hdl.handle.net/11336/23828 |
| Access Level: | acceso abierto |
| Palabra clave: | Benzo[A]Pyrene Benzo[A]Pyrene Metabolites Solid-Phase Extraction Room-Temperature Fluorescence Urine Analysis Asymmetric Least Squares https://purl.org/becyt/ford/1.4 https://purl.org/becyt/ford/1 |
| Sumario: | Benzo[a]pyrene (B[a]P) is a prototypic carcinogenic polycyclic aromatic hydrocarbon (PAH), which requires metabolic activation to produce its detrimental effects. Measurement of B[a]P metabolites in human urine could provide a direct way to assess individual differences in susceptibility to PAH-related cancer. This article focuses on the development of screening methodology for the routine analysis of B[a]P metabolites in urine samples. It explores the solid-surface room-temperature fluorescence (RTF) properties of 3-hydroxybenzo[ a]pyrene, benzo[a]pyrene-trans-9,10-dihydrodiol, benzo[a]pyrene-r-7,t-8,c-9-tetrahydrotriol and benzo[a]pyrene-r-7,t-8,c-9,c-10-tetrahydrotetrol previously extracted from urine samples with octadecyl-silica membranes. Relative standard deviations varying from 2.1% (benzo[a]pyrene-r-7,t-8,c-9-tetrahydrotriol) to 8.6% (3-hydroxy-benzo[a]pyrene) are obtained with the aid of fiber optic probe that eliminates the need for manual optimization of signal intensities. Analytical recoveries from human urine samples varied from 87.5 ± 3.1% (3-hydroxy-benzo[a]pyrene) to 99.8± 2.5% (benzo[a]pyrene-r-7,t-8,c-9,c-10-tetrahydrotetrol). The excellent analytical figures of merit and the simplicity of the experimental procedure demonstrate the potential of this approach for screening biomarkers of PAH exposure in numerous urine samples. |
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