Thioredoxin 1 and glutaredoxin 2 contribute to maintain the phenotype and integrity of neurons following perinatal asphyxia

Background Thioredoxin (Trx) family proteins are crucial mediators of cell functions via regulation of the thiol redox state of various key proteins and the levels of the intracellular second messenger hydrogen peroxide. Their expression, localization and functions are altered in various pathologies...

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Autores: Romero, Juan Ignacio, Hanschmann, Eva Maria, Gellert, Manuela, Eitner, Susanne, Holubiec, Mariana Ines, Blanco Calvo, Eduardo, Lillig, Christopher Horst, Capani, Francisco
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2015
País:Argentina
Institución:Consejo Nacional de Investigaciones Científicas y Técnicas
Repositorio:CONICET Digital (CONICET)
Idioma:inglés
OAI Identifier:oai:ri.conicet.gov.ar:11336/37942
Acceso en línea:http://hdl.handle.net/11336/37942
Access Level:acceso abierto
Palabra clave:Common Carotid Artery Occlusion
Hypoxia
Perinatal Asphyxia
Reoxygenation
Thioredoxin Family of Proteins
https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
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network_acronym_str AR
network_name_str Argentina
repository_id_str
dc.title.none.fl_str_mv Thioredoxin 1 and glutaredoxin 2 contribute to maintain the phenotype and integrity of neurons following perinatal asphyxia
title Thioredoxin 1 and glutaredoxin 2 contribute to maintain the phenotype and integrity of neurons following perinatal asphyxia
spellingShingle Thioredoxin 1 and glutaredoxin 2 contribute to maintain the phenotype and integrity of neurons following perinatal asphyxia
Romero, Juan Ignacio
Common Carotid Artery Occlusion
Hypoxia
Perinatal Asphyxia
Reoxygenation
Thioredoxin Family of Proteins
https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
title_short Thioredoxin 1 and glutaredoxin 2 contribute to maintain the phenotype and integrity of neurons following perinatal asphyxia
title_full Thioredoxin 1 and glutaredoxin 2 contribute to maintain the phenotype and integrity of neurons following perinatal asphyxia
title_fullStr Thioredoxin 1 and glutaredoxin 2 contribute to maintain the phenotype and integrity of neurons following perinatal asphyxia
title_full_unstemmed Thioredoxin 1 and glutaredoxin 2 contribute to maintain the phenotype and integrity of neurons following perinatal asphyxia
title_sort Thioredoxin 1 and glutaredoxin 2 contribute to maintain the phenotype and integrity of neurons following perinatal asphyxia
dc.creator.none.fl_str_mv Romero, Juan Ignacio
Hanschmann, Eva Maria
Gellert, Manuela
Eitner, Susanne
Holubiec, Mariana Ines
Blanco Calvo, Eduardo
Lillig, Christopher Horst
Capani, Francisco
author Romero, Juan Ignacio
author_facet Romero, Juan Ignacio
Hanschmann, Eva Maria
Gellert, Manuela
Eitner, Susanne
Holubiec, Mariana Ines
Blanco Calvo, Eduardo
Lillig, Christopher Horst
Capani, Francisco
author_role author
author2 Hanschmann, Eva Maria
Gellert, Manuela
Eitner, Susanne
Holubiec, Mariana Ines
Blanco Calvo, Eduardo
Lillig, Christopher Horst
Capani, Francisco
author2_role author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Common Carotid Artery Occlusion
Hypoxia
Perinatal Asphyxia
Reoxygenation
Thioredoxin Family of Proteins
https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
topic Common Carotid Artery Occlusion
Hypoxia
Perinatal Asphyxia
Reoxygenation
Thioredoxin Family of Proteins
https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
description Background Thioredoxin (Trx) family proteins are crucial mediators of cell functions via regulation of the thiol redox state of various key proteins and the levels of the intracellular second messenger hydrogen peroxide. Their expression, localization and functions are altered in various pathologies. Here, we have analyzed the impact of Trx family proteins in neuronal development and recovery, following hypoxia/ischemia and reperfusion. Methods We have analyzed the regulation and potential functions of Trx family proteins during hypoxia/ischemia and reoxygenation of the developing brain in both an animal and a cellular model of perinatal asphyxia. We have analyzed the distribution of 14 Trx family and related proteins in the cerebellum, striatum, and hippocampus, three areas of the rat brain that are especially susceptible to hypoxia. Using SH-SY5Y cells subjected to hypoxia and reoxygenation, we have analyzed the functions of some redoxins suggested by the animal experiment. Results and conclusions We have described/discovered a complex, cell-type and tissue-specific expression pattern following the hypoxia/ischemia and reoxygenation. Particularly, Grx2 and Trx1 showed distinct changes during tissue recovery following hypoxia/ischemia and reoxygenation. Silencing of these proteins in SH-SY5Y cells subjected to hypoxia-reoxygenation confirmed that these proteins are required to maintain the normal neuronal phenotype. General significance These findings demonstrate the significance of redox signaling in cellular pathways. Grx2 and Trx1 contribute significantly to neuronal integrity and could be clinically relevant in neuronal damage following perinatal asphyxia and other neuronal disorders.
publishDate 2015
dc.date.none.fl_str_mv 2015-06
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/37942
Romero, Juan Ignacio; Hanschmann, Eva Maria; Gellert, Manuela; Eitner, Susanne; Holubiec, Mariana Ines; et al.; Thioredoxin 1 and glutaredoxin 2 contribute to maintain the phenotype and integrity of neurons following perinatal asphyxia; Elsevier Science; Biochimica et Biophysica Acta- General Subjects; 1850; 6; 6-2015; 1274-1285
0304-4165
CONICET Digital
CONICET
url http://hdl.handle.net/11336/37942
identifier_str_mv Romero, Juan Ignacio; Hanschmann, Eva Maria; Gellert, Manuela; Eitner, Susanne; Holubiec, Mariana Ines; et al.; Thioredoxin 1 and glutaredoxin 2 contribute to maintain the phenotype and integrity of neurons following perinatal asphyxia; Elsevier Science; Biochimica et Biophysica Acta- General Subjects; 1850; 6; 6-2015; 1274-1285
0304-4165
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1016/j.bbagen.2015.02.015
info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S030441651500077X
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier Science
publisher.none.fl_str_mv Elsevier Science
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1799195705830539264
spelling Thioredoxin 1 and glutaredoxin 2 contribute to maintain the phenotype and integrity of neurons following perinatal asphyxiaRomero, Juan IgnacioHanschmann, Eva MariaGellert, ManuelaEitner, SusanneHolubiec, Mariana InesBlanco Calvo, EduardoLillig, Christopher HorstCapani, FranciscoCommon Carotid Artery OcclusionHypoxiaPerinatal AsphyxiaReoxygenationThioredoxin Family of Proteinshttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Background Thioredoxin (Trx) family proteins are crucial mediators of cell functions via regulation of the thiol redox state of various key proteins and the levels of the intracellular second messenger hydrogen peroxide. Their expression, localization and functions are altered in various pathologies. Here, we have analyzed the impact of Trx family proteins in neuronal development and recovery, following hypoxia/ischemia and reperfusion. Methods We have analyzed the regulation and potential functions of Trx family proteins during hypoxia/ischemia and reoxygenation of the developing brain in both an animal and a cellular model of perinatal asphyxia. We have analyzed the distribution of 14 Trx family and related proteins in the cerebellum, striatum, and hippocampus, three areas of the rat brain that are especially susceptible to hypoxia. Using SH-SY5Y cells subjected to hypoxia and reoxygenation, we have analyzed the functions of some redoxins suggested by the animal experiment. Results and conclusions We have described/discovered a complex, cell-type and tissue-specific expression pattern following the hypoxia/ischemia and reoxygenation. Particularly, Grx2 and Trx1 showed distinct changes during tissue recovery following hypoxia/ischemia and reoxygenation. Silencing of these proteins in SH-SY5Y cells subjected to hypoxia-reoxygenation confirmed that these proteins are required to maintain the normal neuronal phenotype. General significance These findings demonstrate the significance of redox signaling in cellular pathways. Grx2 and Trx1 contribute significantly to neuronal integrity and could be clinically relevant in neuronal damage following perinatal asphyxia and other neuronal disorders.Fil: Romero, Juan Ignacio. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Cardiológicas. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones Cardiológicas; ArgentinaFil: Hanschmann, Eva Maria. Universitätsmedizin Greifswald; AlemaniaFil: Gellert, Manuela. Universitätsmedizin Greifswald; AlemaniaFil: Eitner, Susanne. Universitätsmedizin Greifswald; AlemaniaFil: Holubiec, Mariana Ines. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Cardiológicas. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones Cardiológicas; ArgentinaFil: Blanco Calvo, Eduardo. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Cardiológicas. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones Cardiológicas; Argentina. Universidad de Lleida; EspañaFil: Lillig, Christopher Horst. Universitätsmedizin Greifswald; AlemaniaFil: Capani, Francisco. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Cardiológicas. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones Cardiológicas; Argentina. Universidad Argentina "John F. Kennedy"; ArgentinaElsevier Science2015-06info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/37942Romero, Juan Ignacio; Hanschmann, Eva Maria; Gellert, Manuela; Eitner, Susanne; Holubiec, Mariana Ines; et al.; Thioredoxin 1 and glutaredoxin 2 contribute to maintain the phenotype and integrity of neurons following perinatal asphyxia; Elsevier Science; Biochimica et Biophysica Acta- General Subjects; 1850; 6; 6-2015; 1274-12850304-4165CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1016/j.bbagen.2015.02.015info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S030441651500077Xinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2024-05-08T14:00:53Zoai:ri.conicet.gov.ar:11336/37942instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982024-05-08 14:00:54.126CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
score 15,81155

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