Improved method for the determination of wax esters in vegetable oils
In this work a modified International Olive Council (IOC) method for wax determination involving a double-adsorbent layer of silica gel and silver nitrate-impregnated silica gel is presented (SN method). Column chromatography by the SN method did not show retention of wax esters standards with an ev...
| Autores: | , , |
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| Tipo de recurso: | artículo |
| Estado: | Versión publicada |
| Fecha de publicación: | 2012 |
| País: | Argentina |
| Institución: | Consejo Nacional de Investigaciones Científicas y Técnicas |
| Repositorio: | CONICET Digital (CONICET) |
| Idioma: | inglés |
| OAI Identifier: | oai:ri.conicet.gov.ar:11336/42994 |
| Acceso en línea: | http://hdl.handle.net/11336/42994 |
| Access Level: | acceso abierto |
| Palabra clave: | Wax Esters Olive Oil Seed Oils Gas Chromatography https://purl.org/becyt/ford/2.11 https://purl.org/becyt/ford/2 |
| Sumario: | In this work a modified International Olive Council (IOC) method for wax determination involving a double-adsorbent layer of silica gel and silver nitrate-impregnated silica gel is presented (SN method). Column chromatography by the SN method did not show retention of wax esters standards with an even number of carbon atoms (C34-C44), observing recovery percentages higher than 90% even for unsaturated wax esters. All wax fractions were lower by the SN method than by the IOC method, resulting in a percentage decrease in the total wax content (olive oils: 20-50%, crude sunflower oil: 38%, crude soybean oil: 58% and crude grape seed oil: 13%). Olive oils analysed by the SN method showed increases of up to 27% in C40 relative percentage with respect to the IOC method. Additionally, decreases were observed by the SN method in the relative percentages for odd-carbon atom waxes for the seed oils in comparison to the IOC method (crude sunflower oil: 27%, crude soybean oil: 28% and crude grape seed oil: 13%). The main advantages of the proposed modification consist in its easy implementation and a better determination of wax esters (C34-C60) by controlling their complete recovery and removing interfering substances. The method is suitable for quality control and for authentication of olive oil and seed oils as well as in processing monitoring. |
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