Cationic lipid: DNA complexes allow bleomycin uptake by melanoma cells
Bleomycin is a chemotherapeutic agent barely diffusible through the plasmatic membrane. We evaluated DNA/cationic lipids complexes (lipoplexes) as mediators of its uptake in four spontaneous canine melanoma derived cell lines (Ak, Bk, Br and Rkb). Cell survival after lipofection plus or minus bleomy...
| Autores: | , , , , |
|---|---|
| Tipo de recurso: | artículo |
| Estado: | Versión publicada |
| Fecha de publicación: | 2013 |
| País: | Argentina |
| Institución: | Consejo Nacional de Investigaciones Científicas y Técnicas |
| Repositorio: | CONICET Digital (CONICET) |
| Idioma: | inglés |
| OAI Identifier: | oai:ri.conicet.gov.ar:11336/15794 |
| Acceso en línea: | http://hdl.handle.net/11336/15794 |
| Access Level: | acceso abierto |
| Palabra clave: | Canine Melanoma Lipoplexes Spheroids Chemotherapy Bleomycin https://purl.org/becyt/ford/3.4 https://purl.org/becyt/ford/3 |
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Cationic lipid: DNA complexes allow bleomycin uptake by melanoma cells |
| title |
Cationic lipid: DNA complexes allow bleomycin uptake by melanoma cells |
| spellingShingle |
Cationic lipid: DNA complexes allow bleomycin uptake by melanoma cells Gil Cardeza, Maria Lourdes Canine Melanoma Lipoplexes Spheroids Chemotherapy Bleomycin https://purl.org/becyt/ford/3.4 https://purl.org/becyt/ford/3 |
| title_short |
Cationic lipid: DNA complexes allow bleomycin uptake by melanoma cells |
| title_full |
Cationic lipid: DNA complexes allow bleomycin uptake by melanoma cells |
| title_fullStr |
Cationic lipid: DNA complexes allow bleomycin uptake by melanoma cells |
| title_full_unstemmed |
Cationic lipid: DNA complexes allow bleomycin uptake by melanoma cells |
| title_sort |
Cationic lipid: DNA complexes allow bleomycin uptake by melanoma cells |
| dc.creator.none.fl_str_mv |
Gil Cardeza, Maria Lourdes Rossi, Ursula Amaranta Villaverde, Marcela Solange Glikin, Gerardo Claudio Finocchiaro, Liliana Maria Elena |
| author |
Gil Cardeza, Maria Lourdes |
| author_facet |
Gil Cardeza, Maria Lourdes Rossi, Ursula Amaranta Villaverde, Marcela Solange Glikin, Gerardo Claudio Finocchiaro, Liliana Maria Elena |
| author_role |
author |
| author2 |
Rossi, Ursula Amaranta Villaverde, Marcela Solange Glikin, Gerardo Claudio Finocchiaro, Liliana Maria Elena |
| author2_role |
author author author author |
| dc.subject.none.fl_str_mv |
Canine Melanoma Lipoplexes Spheroids Chemotherapy Bleomycin https://purl.org/becyt/ford/3.4 https://purl.org/becyt/ford/3 |
| topic |
Canine Melanoma Lipoplexes Spheroids Chemotherapy Bleomycin https://purl.org/becyt/ford/3.4 https://purl.org/becyt/ford/3 |
| description |
Bleomycin is a chemotherapeutic agent barely diffusible through the plasmatic membrane. We evaluated DNA/cationic lipids complexes (lipoplexes) as mediators of its uptake in four spontaneous canine melanoma derived cell lines (Ak, Bk, Br and Rkb). Cell survival after lipofection plus or minus bleomycin was determined by the acid phosphatase method and the cellular uptake of lipoplexes, carrying the E. coli β-galactosidase gene, was evidenced by SYBR Green I staining. The four cell lines resulted sensitive to the bleomycin/lipoplexes system in both spatial configurations. Survival rates values were lower than 20% in monolayers of the four tested lines and lower than 30% in three lines (Ak, Bk and Rkb) when grown as spheroids. The sensitization to bleomycin depended on lipoplexes in Ak and Rkb while Bk (in both spatial configurations) and Br (as monolayers) were sensitive to bleomycin alone. Although some degree of sensitivity to bleomycin was induced by cationic lipids alone in Ak and Rkb monolayers, the maximal bleomycin effects appeared in the presence of lipoplexes. The sensitization was independent of transcriptional activity. The co-administration of lipoplexes diminished bleomycin IC50: 10-fold in Ak and Rkb monolayers; and sensitized the Ak and Rkb resistant spheroids. The bleomycin cytotoxic effects depended on lipoplexes concentration and diminished when cells were incubated at 8 °C. Our results suggest that lipoplexes sensitize cells to bleomycin, increasing its uptake by an active transport mechanism, such as endocytosis. The bleomycin/lipoplexes system appears as a promising combination of chemotherapy and non-viral cancer gene therapy. |
| publishDate |
2013 |
| dc.date.none.fl_str_mv |
2013-05 |
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info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
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article |
| status_str |
publishedVersion |
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http://hdl.handle.net/11336/15794 Gil Cardeza, Maria Lourdes; Rossi, Ursula Amaranta; Villaverde, Marcela Solange; Glikin, Gerardo Claudio; Finocchiaro, Liliana Maria Elena; Cationic lipid: DNA complexes allow bleomycin uptake by melanoma cells; Elsevier Masson; Biomedicine & Pharmacotherapy = Biomedecine & Pharmacotherapie.; 67; 4; 5-2013; 269-275 0753-3322 |
| url |
http://hdl.handle.net/11336/15794 |
| identifier_str_mv |
Gil Cardeza, Maria Lourdes; Rossi, Ursula Amaranta; Villaverde, Marcela Solange; Glikin, Gerardo Claudio; Finocchiaro, Liliana Maria Elena; Cationic lipid: DNA complexes allow bleomycin uptake by melanoma cells; Elsevier Masson; Biomedicine & Pharmacotherapy = Biomedecine & Pharmacotherapie.; 67; 4; 5-2013; 269-275 0753-3322 |
| dc.language.none.fl_str_mv |
eng |
| language |
eng |
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info:eu-repo/semantics/altIdentifier/doi/10.1016/j.biopha.2013.01.002 info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S075333221300005X |
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info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by-nc-nd/2.5/ar/ |
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openAccess |
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https://creativecommons.org/licenses/by-nc-nd/2.5/ar/ |
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application/pdf application/pdf application/pdf application/pdf application/pdf application/pdf application/pdf application/pdf |
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Elsevier Masson |
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Elsevier Masson |
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reponame:CONICET Digital (CONICET) instname:Consejo Nacional de Investigaciones Científicas y Técnicas |
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Consejo Nacional de Investigaciones Científicas y Técnicas |
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CONICET Digital (CONICET) |
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CONICET Digital (CONICET) |
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CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas |
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dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar |
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1799195491671474176 |
| spelling |
Cationic lipid: DNA complexes allow bleomycin uptake by melanoma cellsGil Cardeza, Maria LourdesRossi, Ursula AmarantaVillaverde, Marcela SolangeGlikin, Gerardo ClaudioFinocchiaro, Liliana Maria ElenaCanine MelanomaLipoplexesSpheroidsChemotherapyBleomycinhttps://purl.org/becyt/ford/3.4https://purl.org/becyt/ford/3Bleomycin is a chemotherapeutic agent barely diffusible through the plasmatic membrane. We evaluated DNA/cationic lipids complexes (lipoplexes) as mediators of its uptake in four spontaneous canine melanoma derived cell lines (Ak, Bk, Br and Rkb). Cell survival after lipofection plus or minus bleomycin was determined by the acid phosphatase method and the cellular uptake of lipoplexes, carrying the E. coli β-galactosidase gene, was evidenced by SYBR Green I staining. The four cell lines resulted sensitive to the bleomycin/lipoplexes system in both spatial configurations. Survival rates values were lower than 20% in monolayers of the four tested lines and lower than 30% in three lines (Ak, Bk and Rkb) when grown as spheroids. The sensitization to bleomycin depended on lipoplexes in Ak and Rkb while Bk (in both spatial configurations) and Br (as monolayers) were sensitive to bleomycin alone. Although some degree of sensitivity to bleomycin was induced by cationic lipids alone in Ak and Rkb monolayers, the maximal bleomycin effects appeared in the presence of lipoplexes. The sensitization was independent of transcriptional activity. The co-administration of lipoplexes diminished bleomycin IC50: 10-fold in Ak and Rkb monolayers; and sensitized the Ak and Rkb resistant spheroids. The bleomycin cytotoxic effects depended on lipoplexes concentration and diminished when cells were incubated at 8 °C. Our results suggest that lipoplexes sensitize cells to bleomycin, increasing its uptake by an active transport mechanism, such as endocytosis. The bleomycin/lipoplexes system appears as a promising combination of chemotherapy and non-viral cancer gene therapy.Fil: Gil Cardeza, Maria Lourdes. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Oncología "Dr. Ángel Roffo". Unidad de Transferencia Genética; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Rossi, Ursula Amaranta. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Oncología "Dr. Ángel Roffo". Unidad de Transferencia Genética; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Villaverde, Marcela Solange. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Oncología "Dr. Ángel Roffo". Unidad de Transferencia Genética; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Glikin, Gerardo Claudio. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Oncología "Dr. Ángel Roffo". Unidad de Transferencia Genética; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Finocchiaro, Liliana Maria Elena. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Oncología "Dr. Ángel Roffo". Unidad de Transferencia Genética; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaElsevier Masson2013-05info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/15794Gil Cardeza, Maria Lourdes; Rossi, Ursula Amaranta; Villaverde, Marcela Solange; Glikin, Gerardo Claudio; Finocchiaro, Liliana Maria Elena; Cationic lipid: DNA complexes allow bleomycin uptake by melanoma cells; Elsevier Masson; Biomedicine & Pharmacotherapy = Biomedecine & Pharmacotherapie.; 67; 4; 5-2013; 269-2750753-3322enginfo:eu-repo/semantics/altIdentifier/doi/10.1016/j.biopha.2013.01.002info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S075333221300005Xinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-nd/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2024-05-08T13:54:12Zoai:ri.conicet.gov.ar:11336/15794instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982024-05-08 13:54:12.361CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse |
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15.811543 |